ID   TREZ_ARTRM              Reviewed;         575 AA.
AC   Q9AJN6;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Malto-oligosyltrehalose trehalohydrolase;
DE            Short=MTHase;
DE            EC=3.2.1.141 {ECO:0000250|UniProtKB:Q55088};
DE   AltName: Full=4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase;
DE   AltName: Full=Maltooligosyl trehalose trehalohydrolase;
GN   Name=treZ;
OS   Arthrobacter ramosus.
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter.
OX   NCBI_TaxID=1672;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S34;
RX   PubMed=11471747; DOI=10.1271/bbb.65.1419;
RA   Yamamoto T., Maruta K., Watanabe H., Yamashita H., Kubota M., Fukuda S.,
RA   Kurimoto M.;
RT   "Trehalose-producing operon treYZ from Arthrobacter ramosus S34.";
RL   Biosci. Biotechnol. Biochem. 65:1419-1423(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-
CC         [(1->4)-alpha-D-glucanosyl]n trehalose to yield trehalose and (1->4)-
CC         alpha-D-glucan.; EC=3.2.1.141;
CC         Evidence={ECO:0000250|UniProtKB:Q55088};
CC   -!- PATHWAY: Glycan biosynthesis; trehalose biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
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DR   EMBL; AB045141; BAB40766.1; -; Genomic_DNA.
DR   PIR; JC7727; JC7727.
DR   AlphaFoldDB; Q9AJN6; -.
DR   SMR; Q9AJN6; -.
DR   CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   UniPathway; UPA00299; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033942; F:4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005992; P:trehalose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.10.760; -; 1.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR012768; Trehalose_TreZ.
DR   InterPro; IPR044901; Trehalose_TreZ_E-set_sf.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   PIRSF; PIRSF006337; Trehalose_TreZ; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   TIGRFAMs; TIGR02402; trehalose_TreZ; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cytoplasm; Glycosidase; Hydrolase.
FT   CHAIN           1..575
FT                   /note="Malto-oligosyltrehalose trehalohydrolase"
FT                   /id="PRO_0000054322"
FT   ACT_SITE        250
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q55088"
FT   ACT_SITE        287
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q55088"
FT   BINDING         248..253
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q55088"
FT   BINDING         312..316
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q55088"
FT   BINDING         381..386
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q55088"
FT   SITE            382
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q55088"
SQ   SEQUENCE   575 AA;  63080 MW;  284A3F20207E228B CRC64;
     MNRRFPVWAP QAAQVTLVVG QGRAELPLTR DENGWWALQQ PWDGGPDLVD YGYLVDGKGP
     FADPRSLRQP RGVHELGREF DPARYAWGDD GWRGRDLTGA VIYELHVGTF TPEGTLDSAI
     RRLDHLVRLG VDAVELLPVN AFNGTHGWGY DGVLWYAVHE PYGGPEAYQR FVDACHARGL
     AVVQDVVYNH LGPSGNHLPD FGPYLGSGAA NTWGDALNLD GPLSDEVRRY IIDNAVYWLR
     DMHADGLRLD AVHALRDARA LHLLEELAAR VDELAGELGR PLTLIAESDL NDPKLIRSRA
     AHGYGLDAQW DDDVHHAVHA NVTGETVGYY ADFGGLGALV KVFQRGWFHD GTWSSFRERH
     HGRPLDPDIP FRRLVAFAQD HDQVGNRAVG DRMSAQVGEG SLAAAAALVL LGPFTPMLFM
     GEEWGARTPW QFFTSHPEPE LGEATARGRI AEFARMGWDP AVVPDPQDPA TFARSHLDWS
     EPEREPHAGL LAFYTDLIAL RRELPVDAPA REVDADEARG VFAFSRGPLR VTVALRPGPV
     GVPEHGGLVL AYGEVRAGAA GLHLDGPGAA IVRLE