TRF_MANSE
ID TRF_MANSE Reviewed; 681 AA.
AC P22297;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Transferrin;
DE Flags: Precursor;
OS Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC Sphingidae; Sphinginae; Sphingini; Manduca.
OX NCBI_TaxID=7130;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 19-52.
RC TISSUE=Fat body;
RX PubMed=2254322; DOI=10.1016/s0021-9258(18)45794-8;
RA Bartfeld N.S., Law J.H.;
RT "Isolation and molecular cloning of transferrin from the tobacco hornworm,
RT Manduca sexta. Sequence similarity to the vertebrate transferrins.";
RL J. Biol. Chem. 265:21684-21691(1990).
CC -!- FUNCTION: Transferrins are iron binding transport proteins which bind
CC Fe(3+) ion in association with the binding of an anion, usually
CC bicarbonate. This transferrin binds only one Fe(3+) ion per protein
CC molecule.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the transferrin family. {ECO:0000255|PROSITE-
CC ProRule:PRU00741}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M62802; AAA29338.1; -; mRNA.
DR PIR; A36500; A36500.
DR PDB; 6WB6; X-ray; 2.05 A; A/B=19-681.
DR PDBsum; 6WB6; -.
DR AlphaFoldDB; P22297; -.
DR SMR; P22297; -.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR InterPro; IPR016357; Transferrin.
DR InterPro; IPR001156; Transferrin-like_dom.
DR InterPro; IPR018195; Transferrin_Fe_BS.
DR Pfam; PF00405; Transferrin; 2.
DR PIRSF; PIRSF002549; Transferrin; 1.
DR PRINTS; PR00422; TRANSFERRIN.
DR SMART; SM00094; TR_FER; 2.
DR PROSITE; PS00205; TRANSFERRIN_LIKE_1; 2.
DR PROSITE; PS00206; TRANSFERRIN_LIKE_2; 1.
DR PROSITE; PS00207; TRANSFERRIN_LIKE_3; 2.
DR PROSITE; PS51408; TRANSFERRIN_LIKE_4; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Ion transport; Iron; Iron transport; Metal-binding; Repeat; Secreted;
KW Signal; Transport.
FT SIGNAL 1..18
FT /evidence="ECO:0000269|PubMed:2254322"
FT CHAIN 19..681
FT /note="Transferrin"
FT /id="PRO_0000035747"
FT DOMAIN 23..364
FT /note="Transferrin-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DOMAIN 371..676
FT /note="Transferrin-like 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT BINDING 75
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT BINDING 108
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT BINDING 134
FT /ligand="hydrogencarbonate"
FT /ligand_id="ChEBI:CHEBI:17544"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT BINDING 138
FT /ligand="hydrogencarbonate"
FT /ligand_id="ChEBI:CHEBI:17544"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT BINDING 140
FT /ligand="hydrogencarbonate"
FT /ligand_id="ChEBI:CHEBI:17544"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT BINDING 141
FT /ligand="hydrogencarbonate"
FT /ligand_id="ChEBI:CHEBI:17544"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT BINDING 222
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT CARBOHYD 218
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 355
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 418
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 26..60
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 35..51
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 132..228
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 181..207
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 204..213
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 271..284
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 374..411
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 384..402
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 478..551
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 506..678
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT DISULFID 579..596
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00741"
FT STRAND 23..28
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 29..31
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 32..39
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 49..56
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 57..65
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 68..74
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 76..83
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 90..97
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 99..101
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 105..115
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 123..126
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 130..132
FT /evidence="ECO:0007829|PDB:6WB6"
FT TURN 139..142
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 143..149
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 165..176
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 177..181
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 186..189
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 190..199
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 201..204
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 207..209
FT /evidence="ECO:0007829|PDB:6WB6"
FT TURN 210..212
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 221..231
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 235..240
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 241..247
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 264..266
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 267..270
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 276..278
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 286..289
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 293..297
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 298..300
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 301..303
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 304..318
FT /evidence="ECO:0007829|PDB:6WB6"
FT TURN 319..321
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 324..328
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 338..345
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 347..352
FT /evidence="ECO:0007829|PDB:6WB6"
FT TURN 353..355
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 356..360
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 365..367
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 370..377
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 378..393
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 397..404
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 408..416
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 421..425
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 429..437
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 440..447
FT /evidence="ECO:0007829|PDB:6WB6"
FT TURN 448..451
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 455..461
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 469..472
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 475..480
FT /evidence="ECO:0007829|PDB:6WB6"
FT TURN 485..488
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 489..497
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 503..505
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 506..513
FT /evidence="ECO:0007829|PDB:6WB6"
FT TURN 514..516
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 517..519
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 536..539
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 548..553
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 560..563
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 564..569
FT /evidence="ECO:0007829|PDB:6WB6"
FT TURN 572..574
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 575..578
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 590..595
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 596..600
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 604..608
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 610..613
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 615..629
FT /evidence="ECO:0007829|PDB:6WB6"
FT TURN 633..635
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 647..650
FT /evidence="ECO:0007829|PDB:6WB6"
FT STRAND 656..659
FT /evidence="ECO:0007829|PDB:6WB6"
FT HELIX 662..677
FT /evidence="ECO:0007829|PDB:6WB6"
SQ SEQUENCE 681 AA; 75223 MW; 131AC74BBC319587 CRC64;
MALKLLTLIA LTCAAANAAK SSYKLCVPAA YMKDCEQMLE VPTKSKVALE CVPARDRVEC
LSFVQQRQAD FVPVDPEDMY VASKIPNQDF VVFQEYRTDE EPDAPFRYEA VIVVHKDLPI
NNLDQLKGLR SCHTGVNRNV GYKIPLTMLM KRAVFPKMND HSISPKENEL KALSTFFAKS
CIVGKWSPDP KTNSAWKSQY SHLCSMCEHP ERCDYPDNYS GYEGALRCLA HNNGEVAFTK
VIFTRKFFGL PVGTTPASPS NENPEEFRYL CVDGSKAPIT GKACSWAARP WQGLIGHNDV
LAKLAPLREK VKQLADSGAA DKPEWFTKVL GLSEKIHHVA DNIPIKPIDY LNKANYTEVI
ERGHGAPELV VRLCVTSNVA LSKCRAMSVF AFSRDIRPIL DCVQENSEDA CLKSVQDNGS
DLASVDDMRV AAAAKKYNLH PVFHEVYGEL KTPNYAVAVV KKGTAYNKID DLRGKKSCHS
SYSTFSGLHA PLFYLINKRA IQSDHCVKNL GEFFSGGSCL PGVDKPENNP SGDDVSKLKK
QCGSDSSAWK CLEEDRGDVA FVSSADLSHF DANQYELLCL NRDAGGRDVL SSFATCNVAM
APSRTWVAAK DFLSDVSIAH TPLSLAQMLA TRPDLFNIYG EFLKNNNVIF NNAAKGLATT
EKLDFEKFKT IHDVISSCGL A
