BUD31_MOUSE
ID BUD31_MOUSE Reviewed; 144 AA.
AC Q6PGH1; E0CX20;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Protein BUD31 homolog;
DE AltName: Full=Protein G10 homolog;
GN Name=Bud31;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in the pre-mRNA splicing process. May play a role as
CC regulator of AR transcriptional activity; may increase AR
CC transcriptional activity. {ECO:0000250|UniProtKB:P41223}.
CC -!- SUBUNIT: Identified in the spliceosome C complex. May interact with AR.
CC {ECO:0000250|UniProtKB:P41223}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P41223}.
CC Note=Detected in chromatin at the promoter of AR target genes.
CC {ECO:0000250|UniProtKB:P41223}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6PGH1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PGH1-2; Sequence=VSP_059351;
CC -!- DOMAIN: Contains a short sequence motif (Phe-Xaa-Xaa-Phe-Tyr) that can
CC bind to AR and may modulate AR activity.
CC {ECO:0000250|UniProtKB:P41223}.
CC -!- SIMILARITY: Belongs to the BUD31 (G10) family. {ECO:0000305}.
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DR EMBL; AC127411; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC057025; AAH57025.1; -; mRNA.
DR CCDS; CCDS19857.1; -. [Q6PGH1-2]
DR CCDS; CCDS80463.1; -. [Q6PGH1-1]
DR RefSeq; NP_001008705.1; NM_001008705.2. [Q6PGH1-2]
DR RefSeq; NP_001297699.1; NM_001310770.1. [Q6PGH1-1]
DR RefSeq; NP_001297700.1; NM_001310771.1. [Q6PGH1-1]
DR AlphaFoldDB; Q6PGH1; -.
DR SMR; Q6PGH1; -.
DR IntAct; Q6PGH1; 1.
DR MINT; Q6PGH1; -.
DR iPTMnet; Q6PGH1; -.
DR PhosphoSitePlus; Q6PGH1; -.
DR EPD; Q6PGH1; -.
DR MaxQB; Q6PGH1; -.
DR PaxDb; Q6PGH1; -.
DR PeptideAtlas; Q6PGH1; -.
DR PRIDE; Q6PGH1; -.
DR ProteomicsDB; 265259; -. [Q6PGH1-1]
DR ProteomicsDB; 334512; -.
DR Antibodypedia; 16150; 244 antibodies from 29 providers.
DR DNASU; 231889; -.
DR Ensembl; ENSMUST00000159018; ENSMUSP00000124999; ENSMUSG00000038722. [Q6PGH1-1]
DR Ensembl; ENSMUST00000160075; ENSMUSP00000125564; ENSMUSG00000038722. [Q6PGH1-1]
DR Ensembl; ENSMUST00000162308; ENSMUSP00000125590; ENSMUSG00000038722. [Q6PGH1-2]
DR Ensembl; ENSMUST00000162594; ENSMUSP00000125738; ENSMUSG00000038722. [Q6PGH1-1]
DR GeneID; 231889; -.
DR KEGG; mmu:231889; -.
DR UCSC; uc009amg.1; mouse. [Q6PGH1-1]
DR CTD; 8896; -.
DR MGI; MGI:2141291; Bud31.
DR VEuPathDB; HostDB:ENSMUSG00000038722; -.
DR eggNOG; KOG3404; Eukaryota.
DR GeneTree; ENSGT00390000014300; -.
DR HOGENOM; CLU_087132_4_0_1; -.
DR InParanoid; Q6PGH1; -.
DR OMA; VRCIQSR; -.
DR OrthoDB; 1236198at2759; -.
DR PhylomeDB; Q6PGH1; -.
DR TreeFam; TF105609; -.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR BioGRID-ORCS; 231889; 22 hits in 57 CRISPR screens.
DR ChiTaRS; Bud31; mouse.
DR PRO; PR:Q6PGH1; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q6PGH1; protein.
DR Bgee; ENSMUSG00000038722; Expressed in indifferent gonad and 269 other tissues.
DR Genevisible; Q6PGH1; MM.
DR GO; GO:0000785; C:chromatin; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISO:MGI.
DR GO; GO:0016922; F:nuclear receptor binding; ISS:UniProtKB.
DR GO; GO:0030374; F:nuclear receptor coactivator activity; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISO:MGI.
DR GO; GO:2000825; P:positive regulation of androgen receptor activity; ISS:UniProtKB.
DR InterPro; IPR018230; BUD31/G10-rel_CS.
DR InterPro; IPR001748; G10.
DR PANTHER; PTHR19411; PTHR19411; 1.
DR Pfam; PF01125; G10; 1.
DR PRINTS; PR00322; G10.
DR PROSITE; PS00997; G10_1; 1.
DR PROSITE; PS00998; G10_2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; mRNA processing; mRNA splicing; Nucleus;
KW Reference proteome; Spliceosome.
FT CHAIN 1..144
FT /note="Protein BUD31 homolog"
FT /id="PRO_0000223627"
FT REGION 59..67
FT /note="Interaction with AR"
FT /evidence="ECO:0000250|UniProtKB:P41223"
FT MOTIF 2..10
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOD_RES 125
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P41223"
FT VAR_SEQ 32..72
FT /note="Missing (in isoform 2)"
FT /id="VSP_059351"
SQ SEQUENCE 144 AA; 17000 MW; 520B8E74C97D0926 CRC64;
MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI
FDLFYKRKAI SRELYEYCIK EGYADKNLIA KWKKQGYENL CCLRCIQTRD TNFGTNCICR
VPKSKLEVGR IIECTHCGCR GCSG
