ID   TRHBN_MYCBO             Reviewed;         136 AA.
AC   P0A593; A0A1R3XYL8; Q10784; X2BHW6;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Group 1 truncated hemoglobin GlbN;
DE            Short=Truncated hemoglobin;
DE            Short=trHbN;
DE   AltName: Full=Hemoglobin-like protein HbN;
GN   Name=glbN; OrderedLocusNames=BQ2027_MB1569C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, AND SUBUNIT.
RC   STRAIN=BCG;
RX   PubMed=10500158; DOI=10.1073/pnas.96.20.11223;
RA   Couture M., Yeh S.R., Wittenberg B.A., Wittenberg J.B., Ouellet Y.,
RA   Rousseau D.L., Guertin M.;
RT   "A cooperative oxygen-binding hemoglobin from Mycobacterium tuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:11223-11228(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Binds oxygen cooperatively with very high affinity (P(50) =
CC       0.013 mmHg at 20 degrees Celsius) because of a fast combination (25
CC       microM(-1).sec(-1)) and a slow dissociation (0.2 sec(-1)) rate.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC       Note=Binds 1 heme group per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:10500158}.
CC   -!- SIMILARITY: Belongs to the truncated hemoglobin family. Group I
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AF130980; AAD28758.1; -; Genomic_DNA.
DR   EMBL; LT708304; SIU00172.1; -; Genomic_DNA.
DR   RefSeq; NP_855221.1; NC_002945.3.
DR   RefSeq; WP_003407730.1; NC_002945.4.
DR   AlphaFoldDB; P0A593; -.
DR   BMRB; P0A593; -.
DR   SMR; P0A593; -.
DR   EnsemblBacteria; SIU00172; SIU00172; BQ2027_MB1569C.
DR   GeneID; 45425525; -.
DR   PATRIC; fig|233413.5.peg.1715; -.
DR   OMA; HQTQFIS; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019825; F:oxygen binding; IEA:InterPro.
DR   GO; GO:0005344; F:oxygen carrier activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.490.10; -; 1.
DR   InterPro; IPR009050; Globin-like_sf.
DR   InterPro; IPR012292; Globin/Proto.
DR   InterPro; IPR019795; Globin_bac-like_CS.
DR   InterPro; IPR001486; Hemoglobin_trunc.
DR   InterPro; IPR016339; Hemoglobin_trunc_I.
DR   Pfam; PF01152; Bac_globin; 1.
DR   PIRSF; PIRSF002030; Globin_Protozoa/Cyanobacteria; 1.
DR   SUPFAM; SSF46458; SSF46458; 1.
DR   PROSITE; PS01213; GLOBIN_FAM_2; 1.
PE   1: Evidence at protein level;
KW   Heme; Iron; Metal-binding; Oxygen transport; Transport.
FT   CHAIN           1..136
FT                   /note="Group 1 truncated hemoglobin GlbN"
FT                   /id="PRO_0000162637"
FT   BINDING         81
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="proximal binding residue"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   136 AA;  14449 MW;  B75D01A45BC064BB CRC64;
     MGLLSRLRKR EPISIYDKIG GHEAIEVVVE DFYVRVLADD QLSAFFSGTN MSRLKGKQVE
     FFAAALGGPE PYTGAPMKQV HQGRGITMHH FSLVAGHLAD ALTAAGVPSE TITEILGVIA
     PLAVDVTSGE STTAPV