BUD31_YEAST
ID BUD31_YEAST Reviewed; 157 AA.
AC P25337; D6VR67;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Pre-mRNA-splicing factor BUD31;
DE AltName: Full=Bud site selection protein 31;
DE AltName: Full=Complexed with CEF1 protein 14;
GN Name=BUD31; Synonyms=CWC14; OrderedLocusNames=YCR063W;
GN ORFNames=YCR63W, YCR903;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1561837; DOI=10.1002/yea.320080209;
RA Benit P., Chanet R., Fabre F., Faye G., Fukuhara H., Sor F.;
RT "Sequence of the sup61-RAD18 region on chromosome III of Saccharomyces
RT cerevisiae.";
RL Yeast 8:147-153(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP IDENTIFICATION IN THE CWC COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
RN [5]
RP INVOLVEMENT IN BUDDING.
RX PubMed=11452010; DOI=10.1091/mbc.12.7.2147;
RA Ni L., Snyder M.;
RT "A genomic study of the bipolar bud site selection pattern in Saccharomyces
RT cerevisiae.";
RL Mol. Biol. Cell 12:2147-2170(2001).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Involved in pre-mRNA splicing. Important for bud site
CC selection.
CC -!- SUBUNIT: Belongs to the CWC complex (or CEF1-associated complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome
CC composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2,
CC CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24,
CC CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9,
CC PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3,
CC SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
CC {ECO:0000269|PubMed:11884590}.
CC -!- INTERACTION:
CC P25337; Q03654: CEF1; NbExp=12; IntAct=EBI-547, EBI-476;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1900 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the BUD31 (G10) family. {ECO:0000305}.
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DR EMBL; X59720; CAA42278.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07536.1; -; Genomic_DNA.
DR PIR; S22261; S22261.
DR RefSeq; NP_009990.1; NM_001178774.1.
DR PDB; 2MY1; NMR; -; A=2-157.
DR PDB; 5GM6; EM; 3.50 A; T=1-157.
DR PDB; 5GMK; EM; 3.40 A; T=1-157.
DR PDB; 5LJ3; EM; 3.80 A; L=1-157.
DR PDB; 5LJ5; EM; 3.80 A; L=1-157.
DR PDB; 5LQW; EM; 5.80 A; E=1-157.
DR PDB; 5MPS; EM; 3.85 A; L=1-157.
DR PDB; 5MQ0; EM; 4.17 A; L=1-157.
DR PDB; 5WSG; EM; 4.00 A; T=1-157.
DR PDB; 5Y88; EM; 3.70 A; L=1-157.
DR PDB; 5YLZ; EM; 3.60 A; L=1-157.
DR PDB; 6BK8; EM; 3.30 A; I=1-157.
DR PDB; 6EXN; EM; 3.70 A; L=1-157.
DR PDB; 6J6G; EM; 3.20 A; T=1-157.
DR PDB; 6J6H; EM; 3.60 A; T=1-157.
DR PDB; 6J6N; EM; 3.86 A; T=1-157.
DR PDB; 6J6Q; EM; 3.70 A; T=1-157.
DR PDBsum; 2MY1; -.
DR PDBsum; 5GM6; -.
DR PDBsum; 5GMK; -.
DR PDBsum; 5LJ3; -.
DR PDBsum; 5LJ5; -.
DR PDBsum; 5LQW; -.
DR PDBsum; 5MPS; -.
DR PDBsum; 5MQ0; -.
DR PDBsum; 5WSG; -.
DR PDBsum; 5Y88; -.
DR PDBsum; 5YLZ; -.
DR PDBsum; 6BK8; -.
DR PDBsum; 6EXN; -.
DR PDBsum; 6J6G; -.
DR PDBsum; 6J6H; -.
DR PDBsum; 6J6N; -.
DR PDBsum; 6J6Q; -.
DR AlphaFoldDB; P25337; -.
DR SMR; P25337; -.
DR BioGRID; 31040; 742.
DR ComplexPortal; CPX-1651; PRP19-associated complex.
DR DIP; DIP-1180N; -.
DR IntAct; P25337; 44.
DR MINT; P25337; -.
DR STRING; 4932.YCR063W; -.
DR MaxQB; P25337; -.
DR PaxDb; P25337; -.
DR PRIDE; P25337; -.
DR EnsemblFungi; YCR063W_mRNA; YCR063W; YCR063W.
DR GeneID; 850427; -.
DR KEGG; sce:YCR063W; -.
DR SGD; S000000659; BUD31.
DR VEuPathDB; FungiDB:YCR063W; -.
DR eggNOG; KOG3404; Eukaryota.
DR GeneTree; ENSGT00390000014300; -.
DR HOGENOM; CLU_087132_1_1_1; -.
DR InParanoid; P25337; -.
DR OMA; VRCIQSR; -.
DR BioCyc; YEAST:G3O-29367-MON; -.
DR PRO; PR:P25337; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P25337; protein.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR GO; GO:0005686; C:U2 snRNP; IDA:SGD.
DR GO; GO:0000282; P:cellular bud site selection; HMP:SGD.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IPI:SGD.
DR InterPro; IPR018230; BUD31/G10-rel_CS.
DR InterPro; IPR001748; G10.
DR PANTHER; PTHR19411; PTHR19411; 1.
DR Pfam; PF01125; G10; 1.
DR PRINTS; PR00322; G10.
DR PROSITE; PS00997; G10_1; 1.
DR PROSITE; PS00998; G10_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome.
FT CHAIN 1..157
FT /note="Pre-mRNA-splicing factor BUD31"
FT /id="PRO_0000193902"
FT MOTIF 2..11
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT STRAND 7..9
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 16..33
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 42..67
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 75..83
FT /evidence="ECO:0007829|PDB:6J6G"
FT STRAND 84..86
FT /evidence="ECO:0007829|PDB:2MY1"
FT HELIX 89..95
FT /evidence="ECO:0007829|PDB:6J6G"
FT TURN 99..102
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 107..109
FT /evidence="ECO:0007829|PDB:6J6G"
FT TURN 111..113
FT /evidence="ECO:0007829|PDB:6J6G"
FT STRAND 114..117
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 121..123
FT /evidence="ECO:0007829|PDB:6J6G"
FT HELIX 126..136
FT /evidence="ECO:0007829|PDB:6J6G"
FT TURN 146..148
FT /evidence="ECO:0007829|PDB:6J6G"
SQ SEQUENCE 157 AA; 18447 MW; 70A8B15F8DA6F85D CRC64;
MPRIKTRRSK PAPDGFEKIK PTLTDFEIQL RDAQKDKSSK LAAKSNEQLW EIMQLHHQRS
RYIYTLYYKR KAISKDLYDW LIKEKYADKL LIAKWRKTGY EKLCCLRCIQ KNETNNGSTC
ICRVPRAQLE EEARKKGTQV SFHQCVHCGC RGCASTD
