ID   BUD4_KLULA              Reviewed;        1217 AA.
AC   Q6CPC2;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Bud site selection protein 4;
GN   Name=BUD4; OrderedLocusNames=KLLA0E05962g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for selection of future bud sites. Cooperates with
CC       other bud site selection proteins to recognize a spatial landmark
CC       during mitosis and they subsequently become a landmark for downstream
CC       polarity establishment factors that coordinate budding and cytokinesis.
CC       Involved in the septin organization at the bud neck (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Bud neck. Note=Localizes to two distinct rings on
CC       either side of the mother-bud neck. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BUD4 family. {ECO:0000305}.
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DR   EMBL; CR382125; CAG99304.1; -; Genomic_DNA.
DR   RefSeq; XP_454217.1; XM_454217.1.
DR   AlphaFoldDB; Q6CPC2; -.
DR   SMR; Q6CPC2; -.
DR   STRING; 28985.XP_454217.1; -.
DR   EnsemblFungi; CAG99304; CAG99304; KLLA0_E06007g.
DR   GeneID; 2894529; -.
DR   KEGG; kla:KLLA0_E06007g; -.
DR   eggNOG; ENOG502REBM; Eukaryota.
DR   HOGENOM; CLU_004727_0_0_1; -.
DR   InParanoid; Q6CPC2; -.
DR   OMA; MLVKHNT; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0005935; C:cellular bud neck; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   Pfam; PF00169; PH; 1.
DR   SMART; SM00233; PH; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Reference proteome.
FT   CHAIN           1..1217
FT                   /note="Bud site selection protein 4"
FT                   /id="PRO_0000330080"
FT   DOMAIN          1086..1195
FT                   /note="PH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT   REGION          430..514
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        435..471
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        475..494
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1217 AA;  138459 MW;  D7CD8A66D63D3805 CRC64;
     MQMEDKGEAL NPDAMYSLLK EIDQEISHTD NISIDQLPEE DTLDNEKKID ASENVIENTE
     EEALQLGDES ANTTTEIKET SCTEDHDTVE NTEDVISMAE GLDKAPLETA WIKHNYQQTK
     PVVSKRLISN DGSDTSVEDI NASTRIPSYT SEEITGLELK DKMVTDVGER LSHFLDQKYA
     ENPNLPALKG MLSMADEKAM EDDPKKDKPL VFTIEPTKPL FPSKEVSSTM IQVEIHTPDE
     DDVDGAAADT KEATNLLLDS KIPVTPNVCI NRFVNNNGET RVSSGSSTED AQELNTHRFQ
     DRYHLLSGAQ AVPQLPQLPA LTLQDYKSSS VQSVNASRIF SVATTNDNYQ SAKEYDLSST
     VGCEELSDDA SVKDLEIPEP NELPCFPTFQ LDSTSMKTSS SMETVQHDKA KFNSDVNIAD
     DTMEQIEQEE AELISNPERS GSPTNDNMSQ HSQNRQLLGV SEVSQAQQAP EHHVEMSVEF
     QDEGRRDITS SFSRESDRIE LPPLPPMNGL STMFDQDLFN DHETSHESID LTSSSHKENY
     LSIWHSQKTG GSFISPALST NSQFSTQSRT SSVPTNHSGS SFKFKSRIIS NSHVFKQEAF
     RQFSDEYVLS TQDDSKLDPL RRNTIMSKRI QQELKTQAKM YPFANKFALD ESSEPSIHNE
     SIITGDISRT SDKAVDTTNI LSHKNLTLLP PQSTETVFSS FLDNFDKDDF EEKLAEESRL
     NSKKNLQTTW GHFRDVSSGN VDIKATQQQI AEVLHNQNED VVQVGSHIQD VKVLLGNEIE
     GYDVQKSVSD LSVETTKKSP IKHVGSPFKV KARSETPPQS PVVYERDTTE VNEGLQRAPT
     TSLASVYLEN SLQEFESSEL QDHGKLYLKL KSINSLRLQQ IKRHNAKYCI EFDNGKEVIE
     TPWESIPEDG PIKMSQEFEV NMESNNVTLF MTMKIRYTSP QNQLTEVVEK IPIKKRFAFG
     KTKYRLEKRF VTKKLKYDDW DFKFAKDGSF ARCQVNVNKN MLKQIKYKTN ELHFEFLNEW
     EREFDEKIAQ TYKEDELWKL PRKNPSKVCS LTVDVMYLPR TSSMERFPKN LKAVQKCCEK
     YLEQQRVNNE GFLWQEGGDV EGMLKRRYMI LKGTELIAHD EVTRKPQTLL NLLNVVDIYS
     DGKTATGKQM RNFTDMVLFS DCFKLLFAND EVINFNADSN LLKQQWVEIL TSVVELNKFH
     QPWIKRVFEN EQYNITF