ID   BUD4_VANPO              Reviewed;        1378 AA.
AC   A7TM88;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Bud site selection protein 4;
GN   Name=BUD4; ORFNames=Kpol_520p2;
OS   Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS
OS   2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17) (Kluyveromyces polysporus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Vanderwaltozyma.
OX   NCBI_TaxID=436907;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL
RC   Y-8283 / UCD 57-17;
RX   PubMed=17494770; DOI=10.1073/pnas.0608218104;
RA   Scannell D.R., Frank A.C., Conant G.C., Byrne K.P., Woolfit M., Wolfe K.H.;
RT   "Independent sorting-out of thousands of duplicated gene pairs in two yeast
RT   species descended from a whole-genome duplication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:8397-8402(2007).
CC   -!- FUNCTION: Required for selection of future bud sites. Cooperates with
CC       other bud site selection proteins to recognize a spatial landmark
CC       during mitosis and they subsequently become a landmark for downstream
CC       polarity establishment factors that coordinate budding and cytokinesis.
CC       Involved in the septin organization at the bud neck (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Bud neck. Note=Localizes to two distinct rings on
CC       either side of the mother-bud neck. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the BUD4 family. {ECO:0000305}.
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DR   EMBL; DS480421; EDO16582.1; -; Genomic_DNA.
DR   RefSeq; XP_001644440.1; XM_001644390.1.
DR   AlphaFoldDB; A7TM88; -.
DR   STRING; 436907.A7TM88; -.
DR   EnsemblFungi; EDO16582; EDO16582; Kpol_520p2.
DR   GeneID; 5544742; -.
DR   KEGG; vpo:Kpol_520p2; -.
DR   eggNOG; ENOG502REBM; Eukaryota.
DR   HOGENOM; CLU_004727_0_0_1; -.
DR   InParanoid; A7TM88; -.
DR   OMA; MLVKHNT; -.
DR   OrthoDB; 290103at2759; -.
DR   PhylomeDB; A7TM88; -.
DR   Proteomes; UP000000267; Unassembled WGS sequence.
DR   GO; GO:0005935; C:cellular bud neck; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR001849; PH_domain.
DR   SMART; SM00233; PH; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Reference proteome.
FT   CHAIN           1..1378
FT                   /note="Bud site selection protein 4"
FT                   /id="PRO_0000330082"
FT   DOMAIN          1240..1357
FT                   /note="PH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT   REGION          71..95
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          361..441
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          458..480
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        361..379
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        380..417
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        466..480
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1378 AA;  155327 MW;  C99500F76C5E20D8 CRC64;
     MIPNDAEGAM DSLLKEIDHE MEVTIGTIDA QEDHSKQKEE TEATNQQTWK LPLQDIGDET
     MDMLVKHNTK NNVINPNFNK KDVDTNESPK NNNDKNVVFN DNVQLLELSA NMDESVLMST
     PTKSDIDISP NKVLINLGDD IANESNEDNK LPLDNKTESE DSDLVSTMLL SKIPITHTEN
     INDFYEKKST DIRASTSRIS SVSSDIEGTP LPTTVQTDQF ELLKQAMKQT FEEDNNSNID
     ISPLKESLLS SELKTKQNLS IEQNEETSVD SIDNSVIHNS EYEVHARSNE REEENENDFE
     NSIEDLEIST HVIEKSPSIE AISTLDPNTD NNKEHFIINN ITKDSTFIEV TSDSKIQIDL
     KDSPENTLHT NEDQKEANTE NVASETNIKE TDQVIHIPTL TEEEISTITN ITNDTETTEE
     GSKAEEDAKN SDVSNSQNSE SINTTITNSL IGIDQNLTNK SSDITNDYEE PPKENELKSS
     PDVIEKGHSS IEHFDDEKIT NSSALGTSNY ISIPLDTLQE ATPVTDSGKT FELQNQDSDN
     INSERIQNLN GSTPVEEKFN ADDELLKTNQ SHITQNKDKS NINEIDNSGT TSIVSDHVVA
     PILPPLPKID KIFIDDPFGE EYDISNDSID LTKSTRPGDY LSIWHIQEEE LKQVSPASTT
     NSQFSNRILS NASSNASAES RISSTAFKFK PRIVSRSRYI NPESRVNSFN ISEEMILPQL
     YGALDPMRRS SRMSRTIQNS IKANRQKSEQ YAGTRKYSIE IQPNQESRIS SRIISNKLEE
     VSSENDKVET MNNSITMDGS FQLLPSFLDN SFGEQLDLTF SKFAKDVLSL RSSSLAQSEI
     HDNSINIWGE DNFIGNTSKN NDHNAMLDSK NKLLEVTDDE KSISESINKD IFADGILKSS
     IFNDITVSRG LNINGLDVDL SDSESVRVSL LTAETDASES GTPIKNTVKD SHVSSPFKVV
     NISKWKNQDG PESDHEVEEI AEIQIEAEKS SESIADISPI KMKPFVDTVV IEEPSQTALV
     DAGSLYLKLK GCLNIKLTGI KHHDARYCFE FDNGRNSIQT AWAVIPSDGD IRVNHDFEST
     VFEVGSKLYV TLKCKYTPPK TEIVEVVEKV LIPKKKLFGK AEYKYEKRFV QRDSKKDEWN
     NLFARDGSFG RAEVLLDHTF LNSVKYNKRN CTFDMMNEWT RLNDKENIEN VFNIPRKPKY
     KVADLKMEVC YLDRVSNKEV FPVSFDKCEM IIKKYKEQLL ISKEGYLMQE GGDVCNNLVR
     RFFKLQGTDL IGYHEVSNLP VVTINMLKVI DVVSSDDLKE EEGKQRSGNS KRNFTDHVLF
     GGCIQLVFTD EEVINLYADT SAQEKIEWYS KLKKTVDLNI CHQPWVTKLA EAQKLEEC