BUD9_YEAST
ID BUD9_YEAST Reviewed; 547 AA.
AC P53226; D6VUH9; Q9HFS8;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Bud site selection protein 9;
GN Name=BUD9; OrderedLocusNames=YGR041W; ORFNames=G4152;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11514631; DOI=10.1091/mbc.12.8.2497;
RA Harkins H.A., Page N., Schenkman L.R., De Virgilio C., Shaw S., Bussey H.,
RA Pringle J.R.;
RT "Bud8p and Bud9p, proteins that may mark the sites for bipolar budding in
RT yeast.";
RL Mol. Biol. Cell 12:2497-2518(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9290212;
RX DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT chromosome VII.";
RL Yeast 13:1077-1090(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP CHARACTERIZATION.
RX PubMed=8657162; DOI=10.1128/mcb.16.4.1857;
RA Zahner J.E., Harkins H.A., Pringle J.R.;
RT "Genetic analysis of the bipolar pattern of bud site selection in the yeast
RT Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 16:1857-1870(1996).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112 AND SER-189, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: May be involved in positioning the proximal bud pole signal.
CC {ECO:0000269|PubMed:11514631}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11514631};
CC Multi-pass membrane protein {ECO:0000269|PubMed:11514631}. Note=Found
CC at the necks of large-budded cells and the proximal poles of daughter
CC cells.
CC -!- PTM: N- and O-glycosylated.
CC -!- MISCELLANEOUS: Present with 952 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: To yeast BUD8. {ECO:0000305}.
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DR EMBL; AF302239; AAG17454.1; -; Genomic_DNA.
DR EMBL; Z72826; CAA97039.1; -; Genomic_DNA.
DR EMBL; Z72827; CAA97041.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08140.1; -; Genomic_DNA.
DR PIR; S64332; S64332.
DR RefSeq; NP_011555.3; NM_001181170.3.
DR AlphaFoldDB; P53226; -.
DR BioGRID; 33288; 108.
DR DIP; DIP-5513N; -.
DR IntAct; P53226; 4.
DR MINT; P53226; -.
DR STRING; 4932.YGR041W; -.
DR iPTMnet; P53226; -.
DR MaxQB; P53226; -.
DR PaxDb; P53226; -.
DR PRIDE; P53226; -.
DR EnsemblFungi; YGR041W_mRNA; YGR041W; YGR041W.
DR GeneID; 852932; -.
DR KEGG; sce:YGR041W; -.
DR SGD; S000003273; BUD9.
DR VEuPathDB; FungiDB:YGR041W; -.
DR eggNOG; ENOG502S1HD; Eukaryota.
DR HOGENOM; CLU_037885_0_0_1; -.
DR InParanoid; P53226; -.
DR OMA; ITTLCIC; -.
DR BioCyc; YEAST:G3O-30761-MON; -.
DR PRO; PR:P53226; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53226; protein.
DR GO; GO:0005935; C:cellular bud neck; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IMP:SGD.
DR GO; GO:0000282; P:cellular bud site selection; IMP:SGD.
PE 1: Evidence at protein level;
KW Cell cycle; Cell membrane; Glycoprotein; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..547
FT /note="Bud site selection protein 9"
FT /id="PRO_0000065021"
FT TOPO_DOM 1..460
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..481
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 482..521
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 522..542
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 543..547
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 123..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..32
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..160
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 214..232
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 326..351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 112
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 189
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CARBOHYD 83
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 221
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 259
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 263
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 289
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 299
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 340
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 16
FT /note="K -> T (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 27
FT /note="A -> T (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 132
FT /note="N -> S (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 249
FT /note="A -> T (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="S -> N (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 298
FT /note="R -> K (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 321
FT /note="S -> N (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 345
FT /note="T -> N (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 406
FT /note="S -> G (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 451
FT /note="G -> D (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 480
FT /note="I -> V (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
FT CONFLICT 545
FT /note="I -> T (in Ref. 1; AAG17454)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 547 AA; 60751 MW; 913D622DAD52C103 CRC64;
MTKITRDVSI TTENSKSTSG SATASSASLP ENDHPIFHQP RARIRSGSLF IEGSDSFPSS
EVKSYNVYID DSKYSEILKG DTNSSSTDGK QVFEDARDDN FHQESHRDLE DSILDLVRRD
PEVAAFPLPP PNSNERNRNS SNGSSAETNL NGHSSSGTIS TSVLLNMGSA EKHAGTTRGD
HMESSSMKSF EKLGTRPSSL FYPPPEGTAP YQGPRATVSG NKSTRQTQGT YSFPSMRYGV
DLVSPVEGAV DVAKSRVPNS TLNGTFPDKA FIPHEFQIPK KAWNRIPANK STSLKTPRNH
SLLIDILKPF EAADLANDQR SSSAVLKNTV HSNGQYNPTN ETSGTRMQDQ RQKNTNEIDL
EKIPNPQVPL GIAMDTMRSP NQLHEKEYES NIEAGLASGV GKGDNSIKQH QYKKIPQEID
RDQQLSFQME TMPIQRIDSS SIRSFDSRIY GFSEIYSIPR VITTLCICLF VPPLFFFFSI
NGNNGVSNYR LMRMIMNYEH RIGLLKGFEW DIDVQWFRTL CFVLGCIEML AIFASIGIGF
GVGIIRE
