TRI12_FUSSP
ID TRI12_FUSSP Reviewed; 598 AA.
AC Q9C1B3;
DT 22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Trichothecene efflux pump TRI12 {ECO:0000303|PubMed:10485289};
DE AltName: Full=Core trichothecene cluster (CTC) protein 12 {ECO:0000303|PubMed:11352533};
GN Name=TRI12 {ECO:0000303|PubMed:10485289};
OS Fusarium sporotrichioides.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=5514;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 24631 / NRRL 3299;
RX PubMed=10485289; DOI=10.1007/s004380051046;
RA Alexander N.J., McCormick S.P., Hohn T.M.;
RT "TRI12, a trichothecene efflux pump from Fusarium sporotrichioides: gene
RT isolation and expression in yeast.";
RL Mol. Gen. Genet. 261:977-984(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 24631 / NRRL 3299;
RX PubMed=11352533; DOI=10.1006/fgbi.2001.1256;
RA Brown D.W., McCormick S.P., Alexander N.J., Proctor R.H., Desjardins A.E.;
RT "A genetic and biochemical approach to study trichothecene diversity in
RT Fusarium sporotrichioides and Fusarium graminearum.";
RL Fungal Genet. Biol. 32:121-133(2001).
RN [3]
RP INDUCTION.
RX PubMed=12732543; DOI=10.1128/aem.69.5.2731-2736.2003;
RA Peplow A.W., Tag A.G., Garifullina G.F., Beremand M.N.;
RT "Identification of new genes positively regulated by Tri10 and a regulatory
RT network for trichothecene mycotoxin production.";
RL Appl. Environ. Microbiol. 69:2731-2736(2003).
CC -!- FUNCTION: Efflux pump that provides the dual role of trichothecene
CC export and self-protection by allowing the fungus to evade the harmful
CC effect of its own trichothecene production (PubMed:10485289,
CC PubMed:11352533). {ECO:0000269|PubMed:10485289,
CC ECO:0000269|PubMed:11352533}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Expression is positively regulated by the trichothecene
CC cluster-specific transcription activator TRI10 (PubMed:12732543).
CC {ECO:0000269|PubMed:12732543}.
CC -!- DISRUPTION PHENOTYPE: Results in both reduced growth on complex media
CC and reduced levels of trichothecene production (PubMed:10485289).
CC {ECO:0000269|PubMed:10485289}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; AF359360; AAK33071.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9C1B3; -.
DR SMR; Q9C1B3; -.
DR TCDB; 2.A.1.3.47; the major facilitator superfamily (mfs).
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR010573; MFS_Str1/Tri12-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF06609; TRI12; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Transmembrane; Transmembrane helix;
KW Transport; Virulence.
FT CHAIN 1..598
FT /note="Trichothecene efflux pump TRI12"
FT /id="PRO_0000442370"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 533..553
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 579..598
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 79
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 161
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 598 AA; 64617 MW; 79C1463337804E5A CRC64;
MTVVVPEEGL DLESQPDDRM RAKALATSAA ELPDGYYRSP RIVASFAAFS MNVVATYFVL
QASASALPNI LQDVGQSENS SLFSTLWTTG QAVSILMMGR LTDRFGRRPF VILTHILGLV
GAIVGCTATK FNTLLAAMTM LGVAAGPAGA SPLFIGELMS NKTKFLGLLI VSAPVVATNG
LSPYLGQRLA IQGSWRWIFY IYIIMSTIAV TLIIIWYYPP SFAQLHGKKV SKREELAKVD
WIGIILVIAG TSLFLLGVSW GGQPNNPWNS AKVIGLISSG AGTLVIFALY EVYGKPERPM
VPPSLFKDTR GFVCILIISS IMGSMHLSLV IMYPQQVVNI FGSSLKNWEE TAWMSATASF
GTGAGVVVLG SLFHLVRHIR WQILVGAMWL TAFLGAMSSI NRDNKNSAIA LSVMTGFVVA
WAQDITMLLV QFITTDENLG VAFAVVAAAR PFAGSIFTAA FISVYTNRYP RELATHLSSA
LRGTGFPQGS FSSLLEAAKS GRMEAVNALP GMTTEISSVV SQAMADSYTA SYANVYYFAM
ALGVIPIIAS LCMRDLDCYL TDHVPHQLYD RKNAHKDVLE GNSESQPSPI ILSMADKE
