ID   TRI12_TRIAR             Reviewed;         602 AA.
AC   G0KYA8;
DT   07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Trichothecene efflux pump TRI12 {ECO:0000303|PubMed:21642405};
DE   AltName: Full=Trichothecene biosynthesis protein 12 {ECO:0000303|PubMed:21642405};
GN   Name=TRI12 {ECO:0000303|PubMed:21642405};
OS   Trichoderma arundinaceum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX   NCBI_TaxID=490622;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IDENTIFICATION.
RC   STRAIN=IBT 40837;
RX   PubMed=21642405; DOI=10.1128/aem.00595-11;
RA   Cardoza R.E., Malmierca M.G., Hermosa M.R., Alexander N.J., McCormick S.P.,
RA   Proctor R.H., Tijerino A.M., Rumbero A., Monte E., Gutierrez S.;
RT   "Identification of loci and functional characterization of trichothecene
RT   biosynthesis genes in filamentous fungi of the genus Trichoderma.";
RL   Appl. Environ. Microbiol. 77:4867-4877(2011).
CC   -!- FUNCTION: Efflux pump that provides the dual role of trichothecene
CC       export and self-protection by allowing the fungus to evade the harmful
CC       effect of its own trichothecene production.
CC       {ECO:0000250|UniProtKB:Q9C1B3}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; FN394492; CAY87358.1; -; Genomic_DNA.
DR   AlphaFoldDB; G0KYA8; -.
DR   SMR; G0KYA8; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR010573; MFS_Str1/Tri12-like.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF06609; TRI12; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Glycoprotein; Membrane; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..602
FT                   /note="Trichothecene efflux pump TRI12"
FT                   /id="PRO_0000445614"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        271..291
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        355..375
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..400
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        408..428
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        450..470
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        484..504
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        532..552
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        160
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        590
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   602 AA;  65528 MW;  B0FA09A1725331AA CRC64;
     MGAPDEVLNI APDEGDKLRA KALAADAEEL PAGYYTSPRV VCTFMSISLT LLSTYFAFEA
     SAAAISFIIE DIGPSENVSL FSTVWTVSQS ISILLMGRLT DRFGRRGFIL GTNCVGIIGG
     IGCLYSFQRF NTMIGAQVLL GLAAGQPGAC ILFIGELMSN KTKFLGNVIV AFPNVIATGF
     GPYIGQSLGI NGNWRWIFYI YIIITAVSTV LAFIFYHPPS FAQLHGKKIS RRDELLKVDW
     IGAFFLTAGM TLFLLGVSWG GSPDPWDSPK ILGLLISGIV SCVIFVLYEC YAKIDRPIIP
     MEFFPGTFAG FGCMLLISGV MGSMNTALFI MYPQQVQHIF SSTLSSWQEV AWMSSTAGFG
     IWAGIVTLGS LFHIFRHIRW QLIFGSAWVT AFLGAMASVN RHKKSEAIAF SICTGFVIGW
     AEDVTMLLVQ FISSDENLGV TFSVVSATRA ICGSIFTAAF ISLYTIKFPG QLQSKLVPAV
     PRRWGFPGVL LVAGFAYWRA LTGQCPSYWL LFPGMTSNLI QVTNDAVADS YAAAYSYVYY
     FAMALGVIAI IASACTKDFD HYLTSHVPHQ IYAAKDADVD LLDSDRSTEN VSSAAVTVSE
     KE