ID   TRI14_TRIAR             Reviewed;         363 AA.
AC   G0KYA7;
DT   07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Trichothecene biosynthesis protein 14 {ECO:0000303|PubMed:21642405};
GN   Name=TRI14 {ECO:0000303|PubMed:21642405};
OS   Trichoderma arundinaceum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX   NCBI_TaxID=490622;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IDENTIFICATION.
RC   STRAIN=IBT 40837;
RX   PubMed=21642405; DOI=10.1128/aem.00595-11;
RA   Cardoza R.E., Malmierca M.G., Hermosa M.R., Alexander N.J., McCormick S.P.,
RA   Proctor R.H., Tijerino A.M., Rumbero A., Monte E., Gutierrez S.;
RT   "Identification of loci and functional characterization of trichothecene
RT   biosynthesis genes in filamentous fungi of the genus Trichoderma.";
RL   Appl. Environ. Microbiol. 77:4867-4877(2011).
RN   [2]
RP   FUNCTION.
RX   PubMed=30121242; DOI=10.1016/j.fgb.2018.08.002;
RA   Lindo L., McCormick S.P., Cardoza R.E., Brown D.W., Kim H.S.,
RA   Alexander N.J., Proctor R.H., Gutierrez S.;
RT   "Effect of deletion of a trichothecene toxin regulatory gene on the
RT   secondary metabolism transcriptome of the saprotrophic fungus Trichoderma
RT   arundinaceum.";
RL   Fungal Genet. Biol. 119:29-46(2018).
CC   -!- FUNCTION: Part of the gene cluster that mediates the production of the
CC       antimicrobial trichothecene harzianum A (HA) that plays a role in
CC       Botrytis cinerea antagonistic activity and plant defense priming
CC       (PubMed:21642405). The biosynthesis of harzianum A begins with the
CC       cyclization of farnesyl diphosphate to trichodiene and is catalyzed by
CC       the trichodiene synthase TRI5 (PubMed:21642405). Trichodiene undergoes
CC       a series of oxygenations catalyzed by the cytochrome P450 monooxygenase
CC       TRI4. TRI4 controls the addition of 3 oxygens at C-2, C-11, and the C-
CC       12, C-13-epoxide to form the intermediate isotrichodiol
CC       (PubMed:21642405). Isotrichodiol then undergoes a non-enzymatic
CC       isomerization and cyclization to form 12,13-epoxytrichothec-9-ene (EPT)
CC       which is further converted to trichodermol by the cytochrome P450
CC       monooxygenase TRI11 via C-4 hydroxylation (PubMed:21642405). The last
CC       step of HA synthesis is esterification of an octatriendioyl moiety to
CC       the C-4 oxygen of trichodermol. The octatriendioyl moiety is probably
CC       produced by the polyketide synthase TRI17 and the esterification
CC       performed by the trichothecene O-acetyltransferase TRI3 (Probable).
CC       {ECO:0000269|PubMed:21642405, ECO:0000305|PubMed:21642405,
CC       ECO:0000305|PubMed:30121242}.
CC   -!- SIMILARITY: Belongs to the TRI14 family. {ECO:0000305}.
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DR   EMBL; FN394491; CAY87357.1; -; Genomic_DNA.
DR   AlphaFoldDB; G0KYA7; -.
DR   SMR; G0KYA7; -.
PE   3: Inferred from homology;
FT   CHAIN           1..363
FT                   /note="Trichothecene biosynthesis protein 14"
FT                   /id="PRO_0000445615"
SQ   SEQUENCE   363 AA;  39961 MW;  4DBFCFB91014C81E CRC64;
     MQPQVLLSSL LPLSDYISGW SWGSLLGNQP CPPLPAGDLV MRKYQMYPEN FMWDKKRCVA
     YVSNLYNATL SIYDPYKSEV IDTISFPGLS HPGDSATPNP LHTSGLILRP DAATADLLEI
     VVDNGDCFFS NGNNVSGPDY LLTMDLRTKK VISQIRLNDI SNGTYAGYAD AELASDGNSY
     VVGTYVSNIL RVTPQSEVST FFVQEPLGPP REYGYTGLAH VGNVLLSNDN IAKQLVRFDI
     RDEKGTPVFI PQTPYHEFTT SNVMNLPEKY NNTILLAAEN VTPDHPSGGV AVWRSRDQLY
     NEVEYLGFIP SRLTNALATA ARQMSDRIYV VSVYTDGANI TVAGYSSEFV LQDITVEVDA
     LVA