TRI45_MOUSE
ID TRI45_MOUSE Reviewed; 580 AA.
AC Q6PFY8; E9Q2K9; Q3U5H4; Q8BVT5;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Tripartite motif-containing protein 45;
GN Name=Trim45;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 3-580 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May act as a transcriptional repressor in mitogen-activated
CC protein kinase signaling pathway.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6PFY8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PFY8-2; Sequence=VSP_012001;
CC -!- SIMILARITY: Belongs to the TRIM/RBCC family. {ECO:0000305}.
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DR EMBL; AK150574; BAE29669.1; -; mRNA.
DR EMBL; AK153571; BAE32104.1; -; mRNA.
DR EMBL; AK076600; BAC36409.1; -; mRNA.
DR EMBL; AL669872; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC057358; AAH57358.1; -; mRNA.
DR CCDS; CCDS17678.1; -. [Q6PFY8-1]
DR CCDS; CCDS51018.1; -. [Q6PFY8-2]
DR RefSeq; NP_001159424.1; NM_001165952.1. [Q6PFY8-1]
DR RefSeq; NP_001159425.1; NM_001165953.1. [Q6PFY8-2]
DR RefSeq; NP_919324.2; NM_194343.2. [Q6PFY8-1]
DR AlphaFoldDB; Q6PFY8; -.
DR SMR; Q6PFY8; -.
DR STRING; 10090.ENSMUSP00000043389; -.
DR iPTMnet; Q6PFY8; -.
DR PhosphoSitePlus; Q6PFY8; -.
DR PaxDb; Q6PFY8; -.
DR PRIDE; Q6PFY8; -.
DR ProteomicsDB; 298219; -. [Q6PFY8-1]
DR ProteomicsDB; 298220; -. [Q6PFY8-2]
DR Antibodypedia; 33887; 241 antibodies from 21 providers.
DR DNASU; 229644; -.
DR Ensembl; ENSMUST00000037409; ENSMUSP00000043389; ENSMUSG00000033233. [Q6PFY8-1]
DR Ensembl; ENSMUST00000106980; ENSMUSP00000102593; ENSMUSG00000033233. [Q6PFY8-1]
DR Ensembl; ENSMUST00000134993; ENSMUSP00000115669; ENSMUSG00000033233. [Q6PFY8-2]
DR GeneID; 229644; -.
DR KEGG; mmu:229644; -.
DR UCSC; uc008qqy.2; mouse. [Q6PFY8-1]
DR UCSC; uc008qqz.2; mouse. [Q6PFY8-2]
DR CTD; 80263; -.
DR MGI; MGI:1918187; Trim45.
DR VEuPathDB; HostDB:ENSMUSG00000033233; -.
DR eggNOG; KOG2177; Eukaryota.
DR GeneTree; ENSGT00940000154334; -.
DR HOGENOM; CLU_013137_14_8_1; -.
DR InParanoid; Q6PFY8; -.
DR OMA; TRCPLCM; -.
DR OrthoDB; 489543at2759; -.
DR TreeFam; TF324196; -.
DR BioGRID-ORCS; 229644; 0 hits in 74 CRISPR screens.
DR ChiTaRS; Ptgfrn; mouse.
DR PRO; PR:Q6PFY8; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q6PFY8; protein.
DR Bgee; ENSMUSG00000033233; Expressed in epithelium of cochlear duct and 165 other tissues.
DR ExpressionAtlas; Q6PFY8; baseline and differential.
DR Genevisible; Q6PFY8; MM.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0045171; C:intercellular bridge; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0060348; P:bone development; IMP:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR003649; Bbox_C.
DR InterPro; IPR017868; Filamin/ABP280_repeat-like.
DR InterPro; IPR001298; Filamin/ABP280_rpt.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR027370; Znf-RING_LisH.
DR InterPro; IPR000315; Znf_B-box.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR Pfam; PF00630; Filamin; 1.
DR Pfam; PF00643; zf-B_box; 1.
DR Pfam; PF13445; zf-RING_UBOX; 1.
DR SMART; SM00502; BBC; 1.
DR SMART; SM00336; BBOX; 2.
DR SMART; SM00557; IG_FLMN; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS50194; FILAMIN_REPEAT; 1.
DR PROSITE; PS50119; ZF_BBOX; 2.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Cytoplasm; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..580
FT /note="Tripartite motif-containing protein 45"
FT /id="PRO_0000056267"
FT REPEAT 394..497
FT /note="Filamin"
FT ZN_FING 29..98
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT ZN_FING 130..176
FT /note="B box-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT ZN_FING 186..227
FT /note="B box-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT COILED 249..329
FT /evidence="ECO:0000255"
FT BINDING 135
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 138
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 158
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 162
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 191
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 194
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 214
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 219
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT VAR_SEQ 532..580
FT /note="GGYLGCGHGHKGHPGRPHWSCCGKFIEKSECSFTSGQGAPRSLLRTVAL ->
FT ARRPKQQQARVRPFAASQVDTLAVATDTKATRAVHTGLAVGSSLRSPSARSRVGRAPRG
FT VCLGPWRSDASWGQVGPGNPEASITSAMQRTQSLSSSKETDRIKDEVLSLTSDFKCFLC
FT GHLSCTMAECRPPLHRSSRQAADLLHGKLSCFIRALRCLCHGTMSSWSREDFMPSFPLC
FT CSLSGLPRARVSGLLLEDETPLAHHLFVVYFQWFPSTQFMMIITVLPSR (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_012001"
FT CONFLICT 250
FT /note="E -> G (in Ref. 3; AAH57358)"
FT /evidence="ECO:0000305"
FT CONFLICT 303
FT /note="R -> L (in Ref. 1; BAC36409)"
FT /evidence="ECO:0000305"
FT CONFLICT Q6PFY8-2:534
FT /note="R -> S (in Ref. 1; BAC36409)"
FT /evidence="ECO:0000305"
FT CONFLICT Q6PFY8-2:535
FT /note="P -> T (in Ref. 1; BAC36409)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 580 AA; 64257 MW; E08709D87D640247 CRC64;
MSEIRKPLLG FVHKLQDANA SGSSGKTHCP TCLRLFKVPR LLPCLHTVCT TCLEKLDPFS
VVDIRGGDSD TSSEGSVFQD PELCSLQPQI GILCPVCDAQ VDLPLGGVKA LTVDHLAMND
VLLENLRGEG QGLVCDLCSD REVEKRCQTC KANLCHFCCQ AHRRQKKTTY HTMVDLKDLK
GYSQVGKPIL CPSHPAEELR LFCELCDRPV CRDCVVGEHR EHPYDFTSNV IHKHGDSVRE
LLRDTQPHVE ALEDALAQIK SVNNALQERV EAVAADVRTF SEGYIKAIEE HRDKLLQQLD
DIRIQRETAL QLQKAQLEQL LADMRTGVEF TEHLLTSGSD LEILITKGVV VERLRKLNKV
EYSARPGVNH KICFSPQEKA GQCQGYEVYG AINTQEVDPA QCVLQGEDLH RAREKQTASF
TLFCKDASGQ SMGRGGDNVH VEVVPKDKKD SPIRTVVQDN KDGSYRVSYT PKEPGIYTVW
VCIREQHVQG SPFNVTVRRK HRPHPGVFHC CTFCSSGGQK AARCACGGTM PGGYLGCGHG
HKGHPGRPHW SCCGKFIEKS ECSFTSGQGA PRSLLRTVAL
