TRI5_FUSAC
ID TRI5_FUSAC Reviewed; 375 AA.
AC Q7LJR6;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 48.
DE RecName: Full=Trichodiene synthase;
DE EC=4.2.3.6;
DE AltName: Full=Sesquiterpene cyclase;
DE Short=TS;
GN Name=TRI5;
OS Fusarium acaciae-mearnsii.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=282272;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CBS 110253 / MRC 2458 / NRRL 26752,
RC CBS 110254 / MRC 5120 / NRRL 26754, and CBS 110255 / MRC 5122 / NRRL 26755;
RX PubMed=12080147; DOI=10.1073/pnas.142307199;
RA Ward T.J., Bielawski J.P., Kistler H.C., Sullivan E., O'Donnell K.;
RT "Ancestral polymorphism and adaptive evolution in the trichothecene
RT mycotoxin gene cluster of phytopathogenic Fusarium.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:9278-9283(2002).
CC -!- FUNCTION: TS is a member of the terpene cyclase group of enzymes. It
CC catalyzes the isomerization and cyclization of farnesyl pyro-phosphate
CC to form trichodiene, the first cyclic intermediate in the biosynthetic
CC pathway for trichothecenes. It serves to branch trichothecene
CC biosynthesis from the isoprenoid pathway.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = diphosphate + trichodiene;
CC Xref=Rhea:RHEA:12052, ChEBI:CHEBI:15861, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:175763; EC=4.2.3.6;
CC -!- PATHWAY: Sesquiterpene biosynthesis; trichothecene biosynthesis.
CC -!- MISCELLANEOUS: Trichothecenes are sesquiterpenoid toxins that act by
CC inhibiting protein biosynthesis.
CC -!- SIMILARITY: Belongs to the trichodiene synthase family. {ECO:0000305}.
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DR EMBL; AY102576; AAM48822.1; -; Genomic_DNA.
DR EMBL; AY102577; AAM48830.1; -; Genomic_DNA.
DR EMBL; AY102578; AAM48838.1; -; Genomic_DNA.
DR AlphaFoldDB; Q7LJR6; -.
DR SMR; Q7LJR6; -.
DR UniPathway; UPA00267; -.
DR GO; GO:0045482; F:trichodiene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IEA:InterPro.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR010458; TRI5_ascomyc.
DR InterPro; IPR024652; Trichodiene_synth.
DR Pfam; PF06330; TRI5; 1.
DR PIRSF; PIRSF001388; TRI5; 1.
DR SFLD; SFLDG01021; Trichodiene_Synthase_Like; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 3: Inferred from homology;
KW Lyase.
FT CHAIN 1..375
FT /note="Trichodiene synthase"
FT /id="PRO_0000221573"
SQ SEQUENCE 375 AA; 43952 MW; 2E05031E6BC860FF CRC64;
MENFPTEYFL NTSVRLLEYI RYRDSNYTRE ERIENLHYAY NKAAHHFAQP RQQKMLKVDP
KRLQASLQTI VGMVVYSWAK VSKECMADLS IHYTYTLVLD DSSDDPHPAM LNYFDDLQAG
REQAHPWWAL VNEHFPNVLR HFGPFCSLNL IRSTMDFFEG CWIEQYNFGG FPGSDDYPQF
LRRMNGLGHC VGASLWPKDL FDERKHFLEI TSAVAQMENW MVWVNDLMSF YKEFDDERDQ
ISLVKNFVTC HEITLDEALE KLTQETLHSS KQMVAVFSDK DPQVMDTIEC FMHGYVTWHL
CDARYRLHEI YEKVKDQDTE DAKKFCKFFE QAANVGAVAP SEWAYPQVAQ LANVRAKDDV
KEAHKPILSS IELVE