TRI5_FUSAU
ID TRI5_FUSAU Reviewed; 375 AA.
AC Q8NIH0;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 58.
DE RecName: Full=Trichodiene synthase;
DE EC=4.2.3.6;
DE AltName: Full=Sesquiterpene cyclase;
DE Short=TS;
GN Name=TRI5;
OS Fusarium austroamericanum.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=282268;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CBS 110244 / NRRL 2903, and CBS 110246 / FRC-R-6964 / NRRL 28718;
RX PubMed=12080147; DOI=10.1073/pnas.142307199;
RA Ward T.J., Bielawski J.P., Kistler H.C., Sullivan E., O'Donnell K.;
RT "Ancestral polymorphism and adaptive evolution in the trichothecene
RT mycotoxin gene cluster of phytopathogenic Fusarium.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:9278-9283(2002).
CC -!- FUNCTION: TS is a member of the terpene cyclase group of enzymes. It
CC catalyzes the isomerization and cyclization of farnesyl pyro-phosphate
CC to form trichodiene, the first cyclic intermediate in the biosynthetic
CC pathway for trichothecenes. It serves to branch trichothecene
CC biosynthesis from the isoprenoid pathway.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = diphosphate + trichodiene;
CC Xref=Rhea:RHEA:12052, ChEBI:CHEBI:15861, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:175763; EC=4.2.3.6;
CC -!- PATHWAY: Sesquiterpene biosynthesis; trichothecene biosynthesis.
CC -!- MISCELLANEOUS: Trichothecenes are sesquiterpenoid toxins that act by
CC inhibiting protein biosynthesis.
CC -!- SIMILARITY: Belongs to the trichodiene synthase family. {ECO:0000305}.
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DR EMBL; AY102589; AAM48926.1; -; Genomic_DNA.
DR EMBL; AY102596; AAM48982.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8NIH0; -.
DR SMR; Q8NIH0; -.
DR UniPathway; UPA00267; -.
DR GO; GO:0045482; F:trichodiene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IEA:InterPro.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR010458; TRI5_ascomyc.
DR InterPro; IPR024652; Trichodiene_synth.
DR Pfam; PF06330; TRI5; 1.
DR PIRSF; PIRSF001388; TRI5; 1.
DR SFLD; SFLDG01021; Trichodiene_Synthase_Like; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 3: Inferred from homology;
KW Lyase.
FT CHAIN 1..375
FT /note="Trichodiene synthase"
FT /id="PRO_0000221575"
SQ SEQUENCE 375 AA; 43975 MW; 4EF324E24F440C26 CRC64;
MENFPTEYFL NTSVRLLEYI RYRDSNYTRE ERIENLHYAY NKAAHHFAQP RQQQMLKVDP
KRLQASLQTI VGMVVYSWAK VSKECMADLS IHYTYTLVLD DSSDDPHPAM LNYFDDLQAG
REQSHPWWAL VNEHFPNVLR HFGPFCSLNL IRSTMDFFEG CWIEQYNFGG FPGSDDYPQF
LRRMNGLGHC VGASLWPKDL FDERKNFLEI TTAVAQMENW MVWVNDLMSF YKEFDDERDQ
ISLVKNFVTC HEITLDEALE KLTQETLHSS KQMVAVFADK DPQVMDTIEC FMHGYVTWHL
CDARYRLHEI YEKVKDQDTE DAKKFCKFFE QAANVGAVAP SEWAYPQVAQ LANVRAKDDM
KEAHKPILSS IELVE