ID   TRI5_FUSPO              Reviewed;         377 AA.
AC   Q00835;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Trichodiene synthase;
DE            EC=4.2.3.6;
DE   AltName: Full=Sesquiterpene cyclase;
DE            Short=TS;
GN   Name=TRI5; Synonyms=TOX5;
OS   Fusarium poae.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX   NCBI_TaxID=36050;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9433809; DOI=10.1023/a:1006882704594;
RA   Fekete C., Logrieco A., Giczey G., Hornok L.;
RT   "Screening of fungi for the presence of the trichodiene synthase encoding
RT   sequence by hybridization to the Tri5 gene cloned from Fusarium poae.";
RL   Mycopathologia 138:91-97(1997).
CC   -!- FUNCTION: TS is a member of the terpene cyclase group of enzymes. It
CC       catalyzes the isomerization and cyclization of farnesyl pyro-phosphate
CC       to form trichodiene, the first cyclic intermediate in the biosynthetic
CC       pathway for trichothecenes. It serves to branch trichothecene
CC       biosynthesis from the isoprenoid pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6E)-farnesyl diphosphate = diphosphate + trichodiene;
CC         Xref=Rhea:RHEA:12052, ChEBI:CHEBI:15861, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:175763; EC=4.2.3.6;
CC   -!- PATHWAY: Sesquiterpene biosynthesis; trichothecene biosynthesis.
CC   -!- MISCELLANEOUS: Trichothecenes are sesquiterpenoid toxins that act by
CC       inhibiting protein biosynthesis.
CC   -!- SIMILARITY: Belongs to the trichodiene synthase family. {ECO:0000305}.
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DR   EMBL; U15658; AAA50765.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q00835; -.
DR   SMR; Q00835; -.
DR   UniPathway; UPA00267; -.
DR   GO; GO:0045482; F:trichodiene synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IEA:InterPro.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR010458; TRI5_ascomyc.
DR   InterPro; IPR024652; Trichodiene_synth.
DR   Pfam; PF06330; TRI5; 1.
DR   PIRSF; PIRSF001388; TRI5; 1.
DR   SFLD; SFLDG01021; Trichodiene_Synthase_Like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   3: Inferred from homology;
KW   Lyase.
FT   CHAIN           1..377
FT                   /note="Trichodiene synthase"
FT                   /id="PRO_0000221582"
SQ   SEQUENCE   377 AA;  44157 MW;  B483DF3CB730F6AB CRC64;
     MENFPTEYFL NTSVRLLEYI RYRDSNYTRE ERIENLHYAY NKAAHHFAQP RQQQLLKVDP
     KRLQASLQTI VGMVVYSWAK VSKECMADLS IHYTYTLVLD DSSDDPYAAM MNYFNDLQAG
     REQAHPWWAL VNEHFPNVLR HFGPFCSLNL IRSTLDFFEG CWIEQYNFGG FPGSHDYPQF
     LRRMNGLGHC VGASLWPKEQ FDERSLFLEI TSAIAQMENW MVWVNDLMSF YKEFDDERDQ
     ISLVKNYVVS DEISLHEALE KLTQDTLHSS KQMVAVFSDK DPQVMVTIEC FMHGYVTWHL
     CDHRYRLNEI SEKVKEQKTE DAQKFCKFYE QAANVGAVSP SEWAYPPVAQ LANVRSKDVK
     NVKQIEKPLL SSIELVE