ID   TRI5_GIBPU              Reviewed;         383 AA.
AC   P27679;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Trichodiene synthase;
DE            EC=4.2.3.6;
DE   AltName: Full=Sesquiterpene cyclase;
DE            Short=TS;
GN   Name=TRI5; Synonyms=TOX5;
OS   Gibberella pulicaris.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX   NCBI_TaxID=5128;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1421511; DOI=10.1094/mpmi-5-249;
RA   Hohn T.M., Desjardins A.E.;
RT   "Isolation and gene disruption of the Tox5 gene encoding trichodiene
RT   synthase in Gibberella pulicaris.";
RL   Mol. Plant Microbe Interact. 5:249-256(1992).
CC   -!- FUNCTION: TS is a member of the terpene cyclase group of enzymes. It
CC       catalyzes the isomerization and cyclization of farnesyl pyro-phosphate
CC       to form trichodiene, the first cyclic intermediate in the biosynthetic
CC       pathway for trichothecenes. It serves to branch trichothecene
CC       biosynthesis from the isoprenoid pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6E)-farnesyl diphosphate = diphosphate + trichodiene;
CC         Xref=Rhea:RHEA:12052, ChEBI:CHEBI:15861, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:175763; EC=4.2.3.6;
CC   -!- PATHWAY: Sesquiterpene biosynthesis; trichothecene biosynthesis.
CC   -!- MISCELLANEOUS: Trichothecenes are sesquiterpenoid toxins that act by
CC       inhibiting protein biosynthesis.
CC   -!- SIMILARITY: Belongs to the trichodiene synthase family. {ECO:0000305}.
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DR   EMBL; M64348; AAB02038.1; -; Genomic_DNA.
DR   AlphaFoldDB; P27679; -.
DR   SMR; P27679; -.
DR   PRIDE; P27679; -.
DR   UniPathway; UPA00267; -.
DR   GO; GO:0045482; F:trichodiene synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IEA:InterPro.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR010458; TRI5_ascomyc.
DR   InterPro; IPR024652; Trichodiene_synth.
DR   Pfam; PF06330; TRI5; 1.
DR   PIRSF; PIRSF001388; TRI5; 1.
DR   SFLD; SFLDG01021; Trichodiene_Synthase_Like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   3: Inferred from homology;
KW   Lyase.
FT   CHAIN           1..383
FT                   /note="Trichodiene synthase"
FT                   /id="PRO_0000221585"
SQ   SEQUENCE   383 AA;  44958 MW;  ABB08F0BCE8662F6 CRC64;
     MENFPTEYFL NTSVRLLEYI RYRDSNYTRE ERIENLHYAY NKAAHHFAQP RQQQLLKVDP
     KRLQASLQTI VGMVVYSWAK VSKECMADLS IHYTYTLVLD DSSDDPYPAM MNYFNDLQAG
     REQAHPWWAL VNEHFPNVLR HFGPFCSLNL IRSTLDFFEG CWIEQYNFGG FPGSHDYPQF
     LRRMNGLGHC VGASLWPKEQ FDERGLFLEI TSAIAQMENW MVWVNDLMSF YKEFDDERDQ
     ISLVKNYVVS DEITLHEALE KLTQDTLHSS KQMVAVFSEK DPQVMDTIEC FMHGYVTWHL
     CDHRYRLNEI YEKVKGQKTE DAEKFCKFYE QAANVGAVSP SEWAYPPIAQ LANIRTKDVK
     DLKDVKDLKE IQKPLLSSIE LVE