TRI5_GIBZE
ID TRI5_GIBZE Reviewed; 375 AA.
AC Q00909; A0A0E0S4D4; Q4IGX1; Q7LJN7; Q7LP63; Q7Z8C3; Q8NII2; Q8NJ97; V6R4Z7;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 2.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Trichodiene synthase;
DE EC=4.2.3.6;
DE AltName: Full=Sesquiterpene cyclase;
DE Short=TS;
GN Name=TRI5; ORFNames=FGRRES_03537, FGSG_03537;
OS Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=229533;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=W-8;
RX PubMed=8589414; DOI=10.1094/mpmi-8-0593;
RA Proctor R.H., Hohn T.M., McCormick S.P.;
RT "Reduced virulence of Gibberella zeae caused by disruption of a
RT trichothecene toxin biosynthetic gene.";
RL Mol. Plant Microbe Interact. 8:593-601(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=H-11;
RX PubMed=11425709; DOI=10.1128/aem.67.7.2966-2972.2001;
RA Lee T., Oh D.W., Kim H.-S., Lee J.-K., Kim Y.-H., Yun S.-H., Lee Y.-W.;
RT "Identification of deoxynivalenol- and nivalenol-producing chemotypes of
RT Gibberella zeae by using PCR.";
RL Appl. Environ. Microbiol. 67:2966-2972(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NRRL 13383, NRRL 28063, NRRL 28336, NRRL 28439, NRRL 29169,
RC NRRL 5883, and NRRL 6394;
RX PubMed=12080147; DOI=10.1073/pnas.142307199;
RA Ward T.J., Bielawski J.P., Kistler H.C., Sullivan E., O'Donnell K.;
RT "Ancestral polymorphism and adaptive evolution in the trichothecene
RT mycotoxin gene cluster of phytopathogenic Fusarium.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:9278-9283(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=IBT1958;
RA Eriksson A.R.B., Schnurer J.;
RT "Trichodiene synthase (Tri5) targeted PCR for identification of Fusarium
RT culmorum, F. graminearum, F. poae and F. sporotrichioides.";
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=GZ3639;
RA Brown D.W., Dyer R.B., Kendra D.F., Plattner R.H.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=17823352; DOI=10.1126/science.1143708;
RA Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT "The Fusarium graminearum genome reveals a link between localized
RT polymorphism and pathogen specialization.";
RL Science 317:1400-1402(2007).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA Hammond-Kosack K.E.;
RT "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT graminearum.";
RL BMC Genomics 16:544-544(2015).
CC -!- FUNCTION: TS is a member of the terpene cyclase group of enzymes. It
CC catalyzes the isomerization and cyclization of farnesyl pyro-phosphate
CC to form trichodiene, the first cyclic intermediate in the biosynthetic
CC pathway for trichothecenes. It serves to branch trichothecene
CC biosynthesis from the isoprenoid pathway.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate = diphosphate + trichodiene;
CC Xref=Rhea:RHEA:12052, ChEBI:CHEBI:15861, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:175763; EC=4.2.3.6;
CC -!- PATHWAY: Sesquiterpene biosynthesis; trichothecene biosynthesis.
CC -!- MISCELLANEOUS: Trichothecenes are sesquiterpenoid toxins that act by
CC inhibiting protein biosynthesis.
CC -!- SIMILARITY: Belongs to the trichodiene synthase family. {ECO:0000305}.
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DR EMBL; U22464; AAB72033.1; -; Genomic_DNA.
DR EMBL; AF336366; AAK53586.1; -; Genomic_DNA.
DR EMBL; AY102567; AAM48750.1; -; Genomic_DNA.
DR EMBL; AY102581; AAM48862.1; -; Genomic_DNA.
DR EMBL; AY102584; AAM48886.1; -; Genomic_DNA.
DR EMBL; AY102587; AAM48910.1; -; Genomic_DNA.
DR EMBL; AY102599; AAM49006.1; -; Genomic_DNA.
DR EMBL; AY102603; AAM49038.1; -; Genomic_DNA.
DR EMBL; AY102605; AAM49054.1; -; Genomic_DNA.
DR EMBL; AF359361; AAK33084.1; -; Genomic_DNA.
DR EMBL; AY130290; AAN05032.1; -; Genomic_DNA.
DR EMBL; DS231664; ESU09673.1; -; Genomic_DNA.
DR EMBL; HG970333; CEF78359.1; -; Genomic_DNA.
DR RefSeq; XP_011322172.1; XM_011323870.1.
DR AlphaFoldDB; Q00909; -.
DR SMR; Q00909; -.
DR STRING; 5518.FGSG_03537P0; -.
DR EnsemblFungi; ESU09673; ESU09673; FGSG_03537.
DR GeneID; 23550840; -.
DR KEGG; fgr:FGSG_03537; -.
DR VEuPathDB; FungiDB:FGRAMPH1_01G13111; -.
DR eggNOG; ENOG502SQ3X; Eukaryota.
DR HOGENOM; CLU_733855_0_0_1; -.
DR InParanoid; Q00909; -.
DR BRENDA; 4.2.3.6; 2428.
DR UniPathway; UPA00267; -.
DR PHI-base; PHI:3518; -.
DR PHI-base; PHI:4243; -.
DR PHI-base; PHI:6797; -.
DR PHI-base; PHI:8390; -.
DR Proteomes; UP000070720; Chromosome 2.
DR GO; GO:0045482; F:trichodiene synthase activity; IMP:PHI-base.
DR GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IEA:InterPro.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR010458; TRI5_ascomyc.
DR InterPro; IPR024652; Trichodiene_synth.
DR Pfam; PF06330; TRI5; 1.
DR PIRSF; PIRSF001388; TRI5; 1.
DR SFLD; SFLDG01021; Trichodiene_Synthase_Like; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 3: Inferred from homology;
KW Lyase; Reference proteome.
FT CHAIN 1..375
FT /note="Trichodiene synthase"
FT /id="PRO_0000221586"
FT VARIANT 54
FT /note="Q -> K (in strain: NRRL 13383 and NRRL 28439)"
FT VARIANT 124
FT /note="A -> S (in strain: NRRL 28336)"
FT VARIANT 133
FT /note="E -> K (in strain: NRRL 29169)"
FT VARIANT 137
FT /note="N -> D (in strain: IBT1958)"
FT VARIANT 206
FT /note="H -> N (in strain: NRRL 28336)"
FT VARIANT 212
FT /note="S -> T (in strain: NRRL 28336)"
FT VARIANT 278
FT /note="S -> A (in strain: NRRL 28336)"
FT VARIANT 317
FT /note="Q -> K (in strain: IBT1958)"
FT VARIANT 360
FT /note="V -> M (in strain: NRRL 28336)"
FT VARIANT 364
FT /note="Q -> H (in strain: NRRL 13383 and NRRL 28439)"
SQ SEQUENCE 375 AA; 43943 MW; 2224743B33FE218D CRC64;
MENFPTEYFL NTSVRLLEYI RYRDSNYTRE ERIENLHYAY NKAAHHFAQP RQQQMLKVDP
KRLQASLQTI VGMVVYSWAK VSKECMADLS IHYTYTLVLD DSSDDPHPAM LNYFDDLQAG
REQAHPWWAL VNEHFPNVLR HFGPFCSLNL IRSTMDFFEG CWIEQYNFGG FPGSDDYPQF
LRRMNGLGHC VGASLWPKDL FDERKHFLEI TSAVAQMENW MVWVNDLMSF YKEFDDERDQ
ISLVKNFVTC HEITLDEALE KLTQETLHSS KQMVAVFSDK DPQVMDTIEC FMHGYVTWHL
CDARYRLHEI YEKVKDQDTE DAKKFCKFFE QAANVGAVAP SEWAYPQVAQ LANVRAKDDV
KEAQKPILSS IELVE
