ID   TRI5_PARRD              Reviewed;         385 AA.
AC   O13489;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Trichodiene synthase;
DE            EC=4.2.3.6;
DE   AltName: Full=Sesquiterpene cyclase;
DE            Short=TS;
GN   Name=TRI5;
OS   Paramyrothecium roridum (Myrothecium leaf spot fungus) (Myrothecium
OS   roridum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Stachybotryaceae; Paramyrothecium.
OX   NCBI_TaxID=1859971;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 52485 / TX 1;
RX   PubMed=9529523; DOI=10.1007/s004380050666;
RA   Trapp S.C., Hohn T.M., McCormick S., Jarvis B.B.;
RT   "Characterization of the gene cluster for biosynthesis of macrocyclic
RT   trichothecenes in Myrothecium roridum.";
RL   Mol. Gen. Genet. 257:421-432(1998).
CC   -!- FUNCTION: TS is a member of the terpene cyclase group of enzymes. It
CC       catalyzes the isomerization and cyclization of farnesyl pyro-phosphate
CC       to form trichodiene, the first cyclic intermediate in the biosynthetic
CC       pathway for trichothecenes. It serves to branch trichothecene
CC       biosynthesis from the isoprenoid pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6E)-farnesyl diphosphate = diphosphate + trichodiene;
CC         Xref=Rhea:RHEA:12052, ChEBI:CHEBI:15861, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:175763; EC=4.2.3.6;
CC   -!- PATHWAY: Sesquiterpene biosynthesis; trichothecene biosynthesis.
CC   -!- MISCELLANEOUS: Trichothecenes are sesquiterpenoid toxins that act by
CC       inhibiting protein biosynthesis.
CC   -!- SIMILARITY: Belongs to the trichodiene synthase family. {ECO:0000305}.
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DR   EMBL; AF009416; AAC49957.1; -; Genomic_DNA.
DR   AlphaFoldDB; O13489; -.
DR   SMR; O13489; -.
DR   PRIDE; O13489; -.
DR   UniPathway; UPA00267; -.
DR   GO; GO:0045482; F:trichodiene synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IEA:InterPro.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR010458; TRI5_ascomyc.
DR   InterPro; IPR024652; Trichodiene_synth.
DR   Pfam; PF06330; TRI5; 1.
DR   PIRSF; PIRSF001388; TRI5; 1.
DR   SFLD; SFLDG01021; Trichodiene_Synthase_Like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   3: Inferred from homology;
KW   Lyase.
FT   CHAIN           1..385
FT                   /note="Trichodiene synthase"
FT                   /id="PRO_0000221587"
SQ   SEQUENCE   385 AA;  44869 MW;  25A492729D5B0D66 CRC64;
     MDEFPTEYFL GTAVRLLENV KYRDSNYTRE ERIENLSYAN NKAAAHFAQE RQQRILKVNP
     KRLEASLRTI VGMVVYSWVK VSKELMADLS IHYTYTLILD DSEDDPHNNM LTFFDDLQAG
     REQKHPWWML VNEHFPNVLR HFGPFCSLNL IRSTLDFFEG CWIEQYNFHG FPGSYDFPGF
     LRRMNGLGHC VGGSLWPKEL FDEQKHFLEI TSAVAQMENW MVWVNDLMSF YKEFDDPRDQ
     TSLVKNYVVC DEISLTQALE KLTVDTLTSS EQMMEVFSDK DAKLMETIEC FMHGYITWHL
     CDHRYRLKEV YEGTMHIETE DAIKFRKFYG QAAKVGAIEH EEWAFPTVAE RIEVRLAEEK
     AAKDGQAVLT SAEPAVPQAA QEVLA