ID   TRI5_TRIHA              Reviewed;         388 AA.
AC   Q6A1B7;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Trichodiene synthase;
DE            EC=4.2.3.6;
DE   AltName: Full=Sesquiterpene cyclase;
DE            Short=TS;
GN   Name=TRI5;
OS   Trichoderma harzianum (Hypocrea lixii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX   NCBI_TaxID=5544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 90237 / F17927;
RX   AGRICOLA=IND43692444; DOI=10.1016/j.pmpp.2004.11.005;
RA   Gallo A., Mulle G., Favilla M., Altomare C.;
RT   "Isolation and characterisation of a trichodiene synthase homologous gene
RT   in Trichoderma harzianum.";
RL   Physiol. Mol. Plant Pathol. 65:11-20(2004).
CC   -!- FUNCTION: TS is a member of the terpene cyclase group of enzymes. It
CC       catalyzes the isomerization and cyclization of farnesyl pyro-phosphate
CC       to form trichodiene, the first cyclic intermediate in the biosynthetic
CC       pathway for trichothecenes. It serves to branch trichothecene
CC       biosynthesis from the isoprenoid pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6E)-farnesyl diphosphate = diphosphate + trichodiene;
CC         Xref=Rhea:RHEA:12052, ChEBI:CHEBI:15861, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:175763; EC=4.2.3.6;
CC   -!- PATHWAY: Sesquiterpene biosynthesis; trichothecene biosynthesis.
CC   -!- MISCELLANEOUS: Trichothecenes are sesquiterpenoid toxins that act by
CC       inhibiting protein biosynthesis.
CC   -!- SIMILARITY: Belongs to the trichodiene synthase family. {ECO:0000305}.
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DR   EMBL; AJ784992; CAH05021.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6A1B7; -.
DR   SMR; Q6A1B7; -.
DR   BRENDA; 4.2.3.6; 6445.
DR   UniPathway; UPA00267; -.
DR   GO; GO:0045482; F:trichodiene synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IEA:InterPro.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR010458; TRI5_ascomyc.
DR   InterPro; IPR024652; Trichodiene_synth.
DR   Pfam; PF06330; TRI5; 1.
DR   PIRSF; PIRSF001388; TRI5; 1.
DR   SFLD; SFLDG01021; Trichodiene_Synthase_Like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   3: Inferred from homology;
KW   Lyase.
FT   CHAIN           1..388
FT                   /note="Trichodiene synthase"
FT                   /id="PRO_0000221589"
SQ   SEQUENCE   388 AA;  44946 MW;  B2FAF74B7473A607 CRC64;
     MVELNDVPGE EEFPRATYLE TMVRLLDTVS YNDENFTDEE RVECLKYAYG KAAEHFAQPH
     VQETLKVPPK RMAAALKTIV GMCVYSWCRV SKEVMADLSI HYTYTLLLDD SREEPAGTMA
     TWYEDLLNAR PQAHGWWRLV NDFIPNVLRH YGGYCQMNMV RSTIDFFQGC WIEQHNFKGF
     RGSSDYPGFL RRINGLGHCV GSSIWPIELV DEEEHFLEIT TAIAQMENWM VWTNDLFSFY
     KEYFAERDQT SLVNNYVECE GITLDQALDK LCKDTIRSSE EIIQVFHDKD PKMYEILTRF
     IQGYITWHLC DDRYRLVEVY EAAGDDPVAQ KFKKYAESAR RVGAIDPARY CVPSVTELCE
     REMAKQSAGR SWDFGLGKIA NKISSVAQ