TRI65_MOUSE
ID TRI65_MOUSE Reviewed; 522 AA.
AC Q8BFW4; A2A867; Q6PGD3;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Tripartite motif-containing protein 65;
GN Name=Trim65;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Liver, and Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BFW4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BFW4-2; Sequence=VSP_020368, VSP_020369;
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DR EMBL; AK028857; BAC26156.1; -; mRNA.
DR EMBL; AK050080; BAC34060.1; -; mRNA.
DR EMBL; AL607108; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC057094; AAH57094.1; -; mRNA.
DR CCDS; CCDS48981.1; -. [Q8BFW4-1]
DR RefSeq; NP_848917.2; NM_178802.4. [Q8BFW4-1]
DR AlphaFoldDB; Q8BFW4; -.
DR SMR; Q8BFW4; -.
DR BioGRID; 237216; 4.
DR STRING; 10090.ENSMUSP00000063410; -.
DR iPTMnet; Q8BFW4; -.
DR PhosphoSitePlus; Q8BFW4; -.
DR EPD; Q8BFW4; -.
DR MaxQB; Q8BFW4; -.
DR PaxDb; Q8BFW4; -.
DR PeptideAtlas; Q8BFW4; -.
DR PRIDE; Q8BFW4; -.
DR ProteomicsDB; 259326; -. [Q8BFW4-1]
DR ProteomicsDB; 259327; -. [Q8BFW4-2]
DR Antibodypedia; 19643; 98 antibodies from 16 providers.
DR DNASU; 338364; -.
DR Ensembl; ENSMUST00000067632; ENSMUSP00000063410; ENSMUSG00000054517. [Q8BFW4-1]
DR Ensembl; ENSMUST00000106440; ENSMUSP00000102048; ENSMUSG00000054517. [Q8BFW4-2]
DR GeneID; 338364; -.
DR KEGG; mmu:338364; -.
DR UCSC; uc007mjz.1; mouse. [Q8BFW4-2]
DR UCSC; uc007mkb.2; mouse. [Q8BFW4-1]
DR CTD; 201292; -.
DR MGI; MGI:2442815; Trim65.
DR VEuPathDB; HostDB:ENSMUSG00000054517; -.
DR eggNOG; KOG2177; Eukaryota.
DR GeneTree; ENSGT00940000161851; -.
DR HOGENOM; CLU_013137_0_2_1; -.
DR InParanoid; Q8BFW4; -.
DR OMA; CTVHECR; -.
DR PhylomeDB; Q8BFW4; -.
DR TreeFam; TF351090; -.
DR BioGRID-ORCS; 338364; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Trim65; mouse.
DR PRO; PR:Q8BFW4; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8BFW4; protein.
DR Bgee; ENSMUSG00000054517; Expressed in aortic valve and 121 other tissues.
DR Genevisible; Q8BFW4; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; ISO:MGI.
DR GO; GO:0046597; P:negative regulation of viral entry into host cell; ISO:MGI.
DR GO; GO:0032897; P:negative regulation of viral transcription; ISO:MGI.
DR GO; GO:0010508; P:positive regulation of autophagy; ISS:UniProtKB.
DR GO; GO:0046598; P:positive regulation of viral entry into host cell; ISO:MGI.
DR GO; GO:0044790; P:suppression of viral release by host; ISO:MGI.
DR Gene3D; 2.60.120.920; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR003879; Butyrophylin_SPRY.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR006574; PRY.
DR InterPro; IPR003877; SPRY_dom.
DR InterPro; IPR018957; Znf_C3HC4_RING-type.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR Pfam; PF00622; SPRY; 1.
DR Pfam; PF00097; zf-C3HC4; 1.
DR PRINTS; PR01407; BUTYPHLNCDUF.
DR SMART; SM00589; PRY; 1.
DR SMART; SM00184; RING; 1.
DR SMART; SM00449; SPRY; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS50188; B302_SPRY; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Coiled coil; Metal-binding;
KW Phosphoprotein; Reference proteome; Zinc; Zinc-finger.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q6PJ69"
FT CHAIN 2..522
FT /note="Tripartite motif-containing protein 65"
FT /id="PRO_0000249192"
FT DOMAIN 316..509
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT ZN_FING 13..52
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT ZN_FING 92..134
FT /note="B box-type"
FT COILED 141..229
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q6PJ69"
FT MOD_RES 187
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 392..396
FT /note="RVSDH -> CVCNA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020368"
FT VAR_SEQ 397..522
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_020369"
SQ SEQUENCE 522 AA; 58458 MW; E42C9B84E8F90B12 CRC64;
MAAQLLEEDV VTCSICLGRY RDPVTLPCGH SFCGNCIQDS WRSCEKSCPE CRQPFPEGAK
LSRNVKMSTL LQALPVLPAP PAVTPRRDSA TSHSARCLRH GRPLEFFCRT EGLCVCSACT
VHDCSHHERA LLDVERRVRE DQLRARVLVT QQQVAQAETQ LQELQEQRSR IESSACTLAS
VVSRRFSSLL QALEKQQAST LSDIEVAKKQ ALGQVLNEKQ RLTDHLRALS QYDQSVQDLL
AQADDCIFFQ ELQQLPEPTE SLGPLTSPQW NEEQQLSNVN QLLSPLCELL LEEKSLPKVA
AKAADAGPLE TLGPLAPVPS AVCPLRKKLW QNYRNLTFDP ETANQYLHLS HKDQRVTHHF
QAQGPAKSGS FELWQVQCTQ SFQTGQHYWE VRVSDHSVTL GVTYPKLSRQ KLGTHTDNIG
RGPCSWGLCI QEDSMQAWHN GKSQRLRGLP GQLLGVDLNL TSGCLTFYSL EPRTQPLHTF
YAVFSQPLFP VFWLLEGRTL TLCHQPEATL PARPQEEATA PS
