ID   TRI6_TRIAR              Reviewed;         218 AA.
AC   G0KYB3;
DT   07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Trichothecene biosynthesis transcription regulator TRI6 {ECO:0000303|PubMed:21642405};
DE   AltName: Full=Trichothecene biosynthesis protein 6 {ECO:0000303|PubMed:21642405};
GN   Name=TRI6 {ECO:0000303|PubMed:21642405};
OS   Trichoderma arundinaceum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX   NCBI_TaxID=490622;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IDENTIFICATION.
RC   STRAIN=IBT 40837;
RX   PubMed=21642405; DOI=10.1128/aem.00595-11;
RA   Cardoza R.E., Malmierca M.G., Hermosa M.R., Alexander N.J., McCormick S.P.,
RA   Proctor R.H., Tijerino A.M., Rumbero A., Monte E., Gutierrez S.;
RT   "Identification of loci and functional characterization of trichothecene
RT   biosynthesis genes in filamentous fungi of the genus Trichoderma.";
RL   Appl. Environ. Microbiol. 77:4867-4877(2011).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=30121242; DOI=10.1016/j.fgb.2018.08.002;
RA   Lindo L., McCormick S.P., Cardoza R.E., Brown D.W., Kim H.S.,
RA   Alexander N.J., Proctor R.H., Gutierrez S.;
RT   "Effect of deletion of a trichothecene toxin regulatory gene on the
RT   secondary metabolism transcriptome of the saprotrophic fungus Trichoderma
RT   arundinaceum.";
RL   Fungal Genet. Biol. 119:29-46(2018).
CC   -!- FUNCTION: Transcriptional activator of part of the trichothecene
CC       biosynthesis cluster that mediates the production of the antimicrobial
CC       trichothecene harzianum A (HA) that plays a role in Botrytis cinerea
CC       antagonistic activity and plant defense priming (PubMed:21642405,
CC       PubMed:30121242). Regulates expression of both trichothecene and
CC       mevalonate pathway genes (PubMed:30121242).
CC       {ECO:0000269|PubMed:21642405, ECO:0000269|PubMed:30121242}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Blocks harzianum A production and reduces
CC       expression of the trichothecene biosynthesis genes, as well as of
CC       mevalonate biosynthetic genes required for synthesis of farnesyl
CC       diphosphate (FPP), the primary metabolite that feeds into trichothecene
CC       biosynthesis (PubMed:30121242). Leads to increased expression of 10 of
CC       49 secondary metabolite (SM) biosynthetic gene clusters in
CC       T.arundinaceum and reduced expression of 5 SM biosynthetic gene
CC       clusters (PubMed:30121242). {ECO:0000269|PubMed:30121242}.
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DR   EMBL; FN394497; CAY87363.1; -; Genomic_DNA.
DR   AlphaFoldDB; G0KYB3; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   SMART; SM00355; ZnF_C2H2; 3.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE   4: Predicted;
KW   Metal-binding; Nucleus; Transcription; Transcription regulation; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..218
FT                   /note="Trichothecene biosynthesis transcription regulator
FT                   TRI6"
FT                   /id="PRO_0000445611"
FT   ZN_FING         185..215
FT                   /note="C2H2-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          154..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        165..181
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   218 AA;  25040 MW;  A9D383616C313C9C CRC64;
     MNDDSETPGA EAWMALPLFN RKASPDHEQN VLTWFECWRP PEDDVSDFKS LEDYEDSPTH
     SIGSSGLHSG SLDSDYHYYT ASDLVFSDDG GVGRNADLLS EISDDHIPQL SFSTPSTCPF
     PGCKSTALFT TGRDFRRHYR QHFKRFFCRY EECSQSTNDP GDAGKKGFAT RKDRARHEAK
     HNPAIRCQWR DNNGDQCTRT FSRMDNMRDH FRRIHGKE