TRI74_HUMAN
ID TRI74_HUMAN Reviewed; 250 AA.
AC Q86UV6; B7WP46;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Tripartite motif-containing protein 74;
DE AltName: Full=Tripartite motif-containing protein 50C;
GN Name=TRIM74; Synonyms=TRIM50C;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=18398435; DOI=10.1038/ejhg.2008.68;
RA Micale L., Fusco C., Augello B., Napolitano L.M.R., Dermitzakis E.T.,
RA Meroni G., Merla G., Reymond A.;
RT "Williams-Beuren syndrome TRIM50 encodes an E3 ubiquitin ligase.";
RL Eur. J. Hum. Genet. 16:1038-1049(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ARG-13.
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC Q86UV6-2; Q03989: ARID5A; NbExp=3; IntAct=EBI-10259086, EBI-948603;
CC Q86UV6-2; P54253: ATXN1; NbExp=6; IntAct=EBI-10259086, EBI-930964;
CC Q86UV6-2; Q9BXY8: BEX2; NbExp=3; IntAct=EBI-10259086, EBI-745073;
CC Q86UV6-2; Q96LT7: C9orf72; NbExp=3; IntAct=EBI-10259086, EBI-2961725;
CC Q86UV6-2; Q96D03: DDIT4L; NbExp=3; IntAct=EBI-10259086, EBI-742054;
CC Q86UV6-2; Q14192: FHL2; NbExp=3; IntAct=EBI-10259086, EBI-701903;
CC Q86UV6-2; P63215: GNG3; NbExp=3; IntAct=EBI-10259086, EBI-1046668;
CC Q86UV6-2; O14964: HGS; NbExp=5; IntAct=EBI-10259086, EBI-740220;
CC Q86UV6-2; O76011: KRT34; NbExp=3; IntAct=EBI-10259086, EBI-1047093;
CC Q86UV6-2; P51608: MECP2; NbExp=3; IntAct=EBI-10259086, EBI-1189067;
CC Q86UV6-2; P19404: NDUFV2; NbExp=3; IntAct=EBI-10259086, EBI-713665;
CC Q86UV6-2; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-10259086, EBI-741158;
CC Q86UV6-2; O43482: OIP5; NbExp=3; IntAct=EBI-10259086, EBI-536879;
CC Q86UV6-2; O14832: PHYH; NbExp=3; IntAct=EBI-10259086, EBI-721853;
CC Q86UV6-2; Q8ND90: PNMA1; NbExp=3; IntAct=EBI-10259086, EBI-302345;
CC Q86UV6-2; P78424: POU6F2; NbExp=3; IntAct=EBI-10259086, EBI-12029004;
CC Q86UV6-2; P0CG20: PRR35; NbExp=3; IntAct=EBI-10259086, EBI-11986293;
CC Q86UV6-2; Q92530: PSMF1; NbExp=3; IntAct=EBI-10259086, EBI-945916;
CC Q86UV6-2; Q04864: REL; NbExp=3; IntAct=EBI-10259086, EBI-307352;
CC Q86UV6-2; Q9BVN2: RUSC1; NbExp=3; IntAct=EBI-10259086, EBI-6257312;
CC Q86UV6-2; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-10259086, EBI-5235340;
CC Q86UV6-2; P51687: SUOX; NbExp=3; IntAct=EBI-10259086, EBI-3921347;
CC Q86UV6-2; Q13148: TARDBP; NbExp=3; IntAct=EBI-10259086, EBI-372899;
CC Q86UV6-2; Q5VWN6: TASOR2; NbExp=3; IntAct=EBI-10259086, EBI-745958;
CC Q86UV6-2; Q5W5X9-3: TTC23; NbExp=3; IntAct=EBI-10259086, EBI-9090990;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q86UV6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q86UV6-2; Sequence=VSP_032087;
CC -!- SIMILARITY: Belongs to the TRIM/RBCC family. {ECO:0000305}.
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DR EMBL; AF498999; AAP30736.1; -; mRNA.
DR EMBL; AC005488; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC033871; AAH33871.1; -; mRNA.
DR CCDS; CCDS5545.1; -. [Q86UV6-1]
DR RefSeq; NP_001304744.1; NM_001317815.1. [Q86UV6-1]
DR RefSeq; NP_942150.1; NM_198853.2. [Q86UV6-1]
DR RefSeq; XP_011514489.1; XM_011516187.2. [Q86UV6-1]
DR RefSeq; XP_011514491.1; XM_011516189.2. [Q86UV6-2]
DR AlphaFoldDB; Q86UV6; -.
DR SMR; Q86UV6; -.
DR BioGRID; 132031; 18.
DR IntAct; Q86UV6; 34.
DR STRING; 9606.ENSP00000285805; -.
DR BioMuta; TRIM74; -.
DR DMDM; 56404879; -.
DR PaxDb; Q86UV6; -.
DR PeptideAtlas; Q86UV6; -.
DR PRIDE; Q86UV6; -.
DR Antibodypedia; 28389; 101 antibodies from 14 providers.
DR DNASU; 378108; -.
DR Ensembl; ENST00000285805.3; ENSP00000285805.3; ENSG00000155428.12. [Q86UV6-1]
DR Ensembl; ENST00000395244.5; ENSP00000378665.1; ENSG00000155428.12. [Q86UV6-2]
DR GeneID; 378108; -.
DR KEGG; hsa:378108; -.
DR MANE-Select; ENST00000285805.3; ENSP00000285805.3; NM_198853.3; NP_942150.1.
DR UCSC; uc003tws.1; human. [Q86UV6-1]
DR CTD; 378108; -.
DR DisGeNET; 378108; -.
DR GeneCards; TRIM74; -.
DR HGNC; HGNC:17453; TRIM74.
DR HPA; ENSG00000155428; Tissue enhanced (stomach, testis).
DR MIM; 612550; gene.
DR neXtProt; NX_Q86UV6; -.
DR OpenTargets; ENSG00000155428; -.
DR PharmGKB; PA38454; -.
DR VEuPathDB; HostDB:ENSG00000155428; -.
DR eggNOG; KOG2177; Eukaryota.
DR GeneTree; ENSGT00940000167700; -.
DR HOGENOM; CLU_013137_6_3_1; -.
DR InParanoid; Q86UV6; -.
DR OMA; HEFIRID; -.
DR OrthoDB; 1096062at2759; -.
DR PhylomeDB; Q86UV6; -.
DR TreeFam; TF342569; -.
DR PathwayCommons; Q86UV6; -.
DR SignaLink; Q86UV6; -.
DR BioGRID-ORCS; 378108; 44 hits in 974 CRISPR screens.
DR ChiTaRS; TRIM74; human.
DR GenomeRNAi; 378108; -.
DR Pharos; Q86UV6; Tdark.
DR PRO; PR:Q86UV6; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q86UV6; protein.
DR Bgee; ENSG00000155428; Expressed in body of stomach and 92 other tissues.
DR Genevisible; Q86UV6; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR027370; Znf-RING_LisH.
DR InterPro; IPR000315; Znf_B-box.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR Pfam; PF00643; zf-B_box; 1.
DR Pfam; PF13445; zf-RING_UBOX; 1.
DR SMART; SM00336; BBOX; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS50119; ZF_BBOX; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Metal-binding; Reference proteome; Zinc;
KW Zinc-finger.
FT CHAIN 1..250
FT /note="Tripartite motif-containing protein 74"
FT /id="PRO_0000056279"
FT ZN_FING 16..57
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT ZN_FING 84..125
FT /note="B box-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT COILED 125..169
FT /evidence="ECO:0000255"
FT COILED 204..235
FT /evidence="ECO:0000255"
FT BINDING 89
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 92
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 111
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 117
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT VAR_SEQ 243
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032087"
FT VARIANT 13
FT /note="W -> R (in dbSNP:rs121966)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_039576"
FT CONFLICT 128
FT /note="V -> I (in Ref. 3; AAH33871)"
FT /evidence="ECO:0000305"
FT CONFLICT 159
FT /note="K -> N (in Ref. 3; AAH33871)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 250 AA; 28547 MW; 6B2F10940B04AC2B CRC64;
MAWQVSLLEL EDWLQCPICL EVFKESLMLQ CGHSYCKGCL VSLSYHLDTK VRCPMCWQVV
DGSSSLPNVS LAWVIEALRL PGDPEPKVCV HHRNPLSLFC EKDQELICGL CGLLGSHQHH
PVTPVSTVCS RMKEELAALF SELKQEQKKV DELIAKLVKN RTRIVNESDV FSWVIRREFQ
ELRHPVDEEK ARCLEGIGGH TRGLVASLDM QLEQAQGTRE RLAQAECVLE QFGNEDHHEF
IWKFHSMASR
