ID   TRIC_TRIVU              Reviewed;         180 AA.
AC   Q29147;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Trichosurin;
DE   Flags: Precursor;
OS   Trichosurus vulpecula (Brush-tailed possum).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Metatheria; Diprotodontia; Phalangeridae; Trichosurus.
OX   NCBI_TaxID=9337;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Mammary gland;
RX   PubMed=9493361; DOI=10.1007/pl00006313;
RA   Piotte C.P., Hunter A.K., Marshall C.J., Grigor M.R.;
RT   "Phylogenetic analysis of three lipocalin-like proteins present in the milk
RT   of Trichosurus vulpecula (Phalangeridae, Marsupialia).";
RL   J. Mol. Evol. 46:361-369(1998).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 16-180, SUBUNIT, AND DISULFIDE
RP   BOND.
RX   PubMed=17685895; DOI=10.1042/bj20070567;
RA   Watson R.P., Demmer J., Baker E.N., Arcus V.L.;
RT   "Three-dimensional structure and ligand binding properties of trichosurin,
RT   a metatherian lipocalin from the milk whey of the common brushtail possum
RT   Trichosurus vulpecula.";
RL   Biochem. J. 408:29-38(2007).
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:17685895}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Milk.
CC   -!- SIMILARITY: Belongs to the calycin superfamily. Lipocalin family.
CC       {ECO:0000305}.
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DR   EMBL; U40376; AAA84739.1; -; mRNA.
DR   PDB; 2R73; X-ray; 2.50 A; A/B/C/D=16-180.
DR   PDB; 2R74; X-ray; 1.90 A; A/B=16-180.
DR   PDB; 2RA6; X-ray; 1.50 A; A/B/C/D=16-180.
DR   PDBsum; 2R73; -.
DR   PDBsum; 2R74; -.
DR   PDBsum; 2RA6; -.
DR   AlphaFoldDB; Q29147; -.
DR   SMR; Q29147; -.
DR   EvolutionaryTrace; Q29147; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0036094; F:small molecule binding; IEA:InterPro.
DR   Gene3D; 2.40.128.20; -; 1.
DR   InterPro; IPR012674; Calycin.
DR   InterPro; IPR002345; Lipocalin.
DR   InterPro; IPR000566; Lipocln_cytosolic_FA-bd_dom.
DR   InterPro; IPR002971; Maj_urinary.
DR   PANTHER; PTHR11430; PTHR11430; 1.
DR   Pfam; PF00061; Lipocalin; 1.
DR   PRINTS; PR01221; MAJORURINARY.
DR   SUPFAM; SSF50814; SSF50814; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Glycoprotein; Milk protein; Secreted; Signal;
KW   Transport.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..180
FT                   /note="Trichosurin"
FT                   /id="PRO_0000017989"
FT   CARBOHYD        67
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        148
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        87..180
FT                   /evidence="ECO:0000269|PubMed:17685895"
FT   STRAND          37..41
FT                   /evidence="ECO:0007829|PDB:2R73"
FT   STRAND          43..51
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   HELIX           52..54
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   STRAND          64..71
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   STRAND          74..83
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   STRAND          86..96
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   STRAND          102..116
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   TURN            120..122
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   STRAND          123..132
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   STRAND          135..147
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   HELIX           151..162
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   TURN            163..165
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   HELIX           168..170
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   STRAND          171..173
FT                   /evidence="ECO:0007829|PDB:2RA6"
FT   HELIX           174..176
FT                   /evidence="ECO:0007829|PDB:2RA6"
SQ   SEQUENCE   180 AA;  21025 MW;  012E1B0F2B39B0E1 CRC64;
     MKLLLLSMGL ALVCGLQPEC SRSEEDLSDE KERKWEQLSR HWHTVVLASS DRSLIEEEGP
     FRNFIQNITV ESGNLNGFFL TRKNGQCIPL YLTAFKTEEA RQFKLNYYGT NDVYYGSSKP
     NEYAKFIFYN YHDGKVNVVA NLFGRTPNLS NEIKKRFEED FMNRGFRREN ILDISEVDHC