ID   TRIC_VIOAR              Reviewed;         204 AA.
AC   P0C589;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Pro-tricyclons;
DE   Contains:
DE     RecName: Full=Tricyclon-A;
DE   Contains:
DE     RecName: Full=Tricyclon-B;
DE   Flags: Precursor;
OS   Viola arvensis (European field pansy) (Field violet).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Violaceae; Viola.
OX   NCBI_TaxID=97415;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE, FUNCTION, STRUCTURE BY
RP   NMR OF 73-105, DISULFIDE BONDS, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=15893660; DOI=10.1016/j.str.2005.02.013;
RA   Mulvenna J.P., Sando L., Craik D.J.;
RT   "Processing of a 22 kDa precursor protein to produce the circular protein
RT   tricyclon A.";
RL   Structure 13:691-701(2005).
CC   -!- FUNCTION: Probably participates in a plant defense mechanism. Has a
CC       weak hemolytic activity. {ECO:0000269|PubMed:15893660}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines those proteins as knottins.
CC   -!- PTM: Proteolytically processed in two steps to yield first two linear
CC       precursors, and then two cyclic peptides after removal of the N-
CC       terminal repeats (NTR) and short tails.
CC   -!- MISCELLANEOUS: Tricyclon-A and tricyclon-B are hybrids of the Bracelet
CC       and Moebius subfamilies.
CC   -!- SIMILARITY: Belongs to the cyclotide family. {ECO:0000305}.
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DR   PDB; 1YP8; NMR; -; A=79-105.
DR   PDBsum; 1YP8; -.
DR   AlphaFoldDB; P0C589; -.
DR   SMR; P0C589; -.
DR   EvolutionaryTrace; P0C589; -.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR005535; Cyclotide.
DR   InterPro; IPR012324; Cyclotide_moebius_CS.
DR   InterPro; IPR036146; Cyclotide_sf.
DR   Pfam; PF03784; Cyclotide; 2.
DR   SUPFAM; SSF57038; SSF57038; 2.
DR   PROSITE; PS60009; CYCLOTIDE_MOEBIUS; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Cytolysis; Direct protein sequencing; Disulfide bond;
KW   Hemolysis; Knottin; Plant defense; Repeat; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..204
FT                   /note="Pro-tricyclons"
FT                   /id="PRO_0000295770"
FT   PROPEP          23..72
FT                   /note="NTR"
FT                   /id="PRO_0000295771"
FT   PEPTIDE         73..105
FT                   /note="Tricyclon-A"
FT                   /id="PRO_0000295772"
FT   PROPEP          106..118
FT                   /id="PRO_0000295773"
FT   PROPEP          119..167
FT                   /note="NTR"
FT                   /id="PRO_0000295774"
FT   PEPTIDE         168..200
FT                   /note="Tricyclon-B"
FT                   /id="PRO_0000295775"
FT   PROPEP          201..204
FT                   /id="PRO_0000295776"
FT   DISULFID        79..93
FT                   /evidence="ECO:0000269|PubMed:15893660"
FT   DISULFID        83..95
FT                   /evidence="ECO:0000269|PubMed:15893660"
FT   DISULFID        88..101
FT                   /evidence="ECO:0000269|PubMed:15893660"
FT   DISULFID        174..188
FT                   /evidence="ECO:0000250"
FT   DISULFID        178..190
FT                   /evidence="ECO:0000250"
FT   DISULFID        183..196
FT                   /evidence="ECO:0000250"
FT   CROSSLNK        73..105
FT                   /note="Cyclopeptide (Gly-Asn)"
FT   CROSSLNK        168..200
FT                   /note="Cyclopeptide (Gly-Asn)"
FT   STRAND          84..86
FT                   /evidence="ECO:0007829|PDB:1YP8"
FT   STRAND          93..96
FT                   /evidence="ECO:0007829|PDB:1YP8"
FT   TURN            97..99
FT                   /evidence="ECO:0007829|PDB:1YP8"
FT   STRAND          100..105
FT                   /evidence="ECO:0007829|PDB:1YP8"
SQ   SEQUENCE   204 AA;  21820 MW;  76EAFD7DB07329C4 CRC64;
     MKTMASFVLV LVAAFALPAA FASLETKDVI TRAAYEKLVE SGAIQGITMT KTIISNPILE
     EALVAHFNRK LGGGTIFDCG ESCFLGTCYT KGCSCGEWKL CYGTNSLPES NNEKAMVASL
     EKDVITRAAY ENLVNSGAIQ GITMTKTIIS NPILEEALVS HFNRKLGGGT IFDCGESCFL
     GTCYTKGCSC GEWKLCYGEN SLAI