TRIL_MOUSE
ID TRIL_MOUSE Reviewed; 809 AA.
AC Q9DBY4; Q80TV0; Q8BKM5;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=TLR4 interactor with leucine rich repeats;
DE AltName: Full=Leucine-rich repeat-containing protein KIAA0644;
DE Flags: Precursor;
GN Name=Tril; Synonyms=Kiaa0644;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Lung, and Spinal ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INDUCTION BY LPS, AND TISSUE SPECIFICITY.
RX PubMed=19710467; DOI=10.4049/jimmunol.0901518;
RA Carpenter S., Carlson T., Dellacasagrande J., Garcia A., Gibbons S.,
RA Hertzog P., Lyons A., Lin L.L., Lynch M., Monie T., Murphy C., Seidl K.J.,
RA Wells C., Dunne A., O'Neill L.A.;
RT "TRIL, a functional component of the TLR4 signaling complex, highly
RT expressed in brain.";
RL J. Immunol. 183:3989-3995(2009).
CC -!- FUNCTION: Component of the TLR4 signaling complex. Mediates the innate
CC immune response to bacterial lipopolysaccharide (LPS) leading to
CC cytokine secretion (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Belongs to the lipopolysaccharide (LPS) receptor, a multi-
CC protein complex containing at least CD14, MD-2 and TLR4. Interacts with
CC TLR4; this interaction is greatly enhanced by LPS stimulation (By
CC similarity). Interacts with LPS (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in brain, spinal cord and lung.
CC {ECO:0000269|PubMed:19710467}.
CC -!- INDUCTION: By bacterial lipopolysaccharides (LPS) (in vivo and in
CC vitro). {ECO:0000269|PubMed:19710467}.
CC -!- PTM: N-glycolysaled. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65620.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK122338; BAC65620.1; ALT_FRAME; mRNA.
DR EMBL; AK004681; BAB23469.1; -; mRNA.
DR EMBL; AK033401; BAC28270.1; -; mRNA.
DR EMBL; AK051421; BAC34634.1; -; mRNA.
DR EMBL; BC043099; AAH43099.1; -; mRNA.
DR EMBL; BC059224; AAH59224.1; -; mRNA.
DR CCDS; CCDS51776.1; -.
DR RefSeq; NP_080093.1; NM_025817.4.
DR AlphaFoldDB; Q9DBY4; -.
DR SMR; Q9DBY4; -.
DR STRING; 10090.ENSMUSP00000116056; -.
DR GlyConnect; 2770; 1 N-Linked glycan (1 site).
DR GlyGen; Q9DBY4; 4 sites, 1 N-linked glycan (1 site).
DR iPTMnet; Q9DBY4; -.
DR PhosphoSitePlus; Q9DBY4; -.
DR MaxQB; Q9DBY4; -.
DR PaxDb; Q9DBY4; -.
DR PRIDE; Q9DBY4; -.
DR ProteomicsDB; 258847; -.
DR Antibodypedia; 73340; 20 antibodies from 9 providers.
DR DNASU; 66873; -.
DR Ensembl; ENSMUST00000127748; ENSMUSP00000116056; ENSMUSG00000043496.
DR GeneID; 66873; -.
DR KEGG; mmu:66873; -.
DR UCSC; uc009bzk.2; mouse.
DR CTD; 9865; -.
DR MGI; MGI:1914123; Tril.
DR VEuPathDB; HostDB:ENSMUSG00000043496; -.
DR eggNOG; KOG0619; Eukaryota.
DR GeneTree; ENSGT00940000161975; -.
DR HOGENOM; CLU_357128_0_0_1; -.
DR InParanoid; Q9DBY4; -.
DR OMA; PHDVLTY; -.
DR OrthoDB; 826997at2759; -.
DR PhylomeDB; Q9DBY4; -.
DR TreeFam; TF331598; -.
DR BioGRID-ORCS; 66873; 5 hits in 71 CRISPR screens.
DR ChiTaRS; Tril; mouse.
DR PRO; PR:Q9DBY4; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9DBY4; protein.
DR Bgee; ENSMUSG00000043496; Expressed in metanephric mesenchyme and 208 other tissues.
DR Genevisible; Q9DBY4; MM.
DR GO; GO:0031012; C:extracellular matrix; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0046696; C:lipopolysaccharide receptor complex; ISO:MGI.
DR GO; GO:0001530; F:lipopolysaccharide binding; ISO:MGI.
DR GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0002718; P:regulation of cytokine production involved in immune response; ISO:MGI.
DR GO; GO:0034142; P:toll-like receptor 4 signaling pathway; ISO:MGI.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR000483; Cys-rich_flank_reg_C.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13855; LRR_8; 4.
DR Pfam; PF01463; LRRCT; 1.
DR SMART; SM00369; LRR_TYP; 11.
DR SUPFAM; SSF49265; SSF49265; 1.
DR PROSITE; PS51450; LRR; 13.
PE 2: Evidence at transcript level;
KW Glycoprotein; Immunity; Inflammatory response; Innate immunity;
KW Leucine-rich repeat; Membrane; Phosphoprotein; Reference proteome; Repeat;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..809
FT /note="TLR4 interactor with leucine rich repeats"
FT /id="PRO_0000349256"
FT TOPO_DOM 26..694
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 695..715
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 716..809
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 26..57
FT /note="LRRNT"
FT REPEAT 61..81
FT /note="LRR 1"
FT REPEAT 84..105
FT /note="LRR 2"
FT REPEAT 108..129
FT /note="LRR 3"
FT REPEAT 132..153
FT /note="LRR 4"
FT REPEAT 156..177
FT /note="LRR 5"
FT REPEAT 180..201
FT /note="LRR 6"
FT REPEAT 204..223
FT /note="LRR 7"
FT REPEAT 230..251
FT /note="LRR 8"
FT REPEAT 254..275
FT /note="LRR 9"
FT REPEAT 278..298
FT /note="LRR 10"
FT REPEAT 302..323
FT /note="LRR 11"
FT REPEAT 326..347
FT /note="LRR 12"
FT DOMAIN 359..416
FT /note="LRRCT"
FT REGION 412..462
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 483..563
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 412..458
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 519..563
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 796
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q496Z2"
FT CARBOHYD 73
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 411
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 587
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 809 AA; 88810 MW; 5E86F55B8AE419FA CRC64;
MEGVGAVRFW LVVCGCLAFP PRAESVCPER CDCQHPQHLL CTNRGLRAVP KTSSLPSPQD
VLTYSLGGNF ITNITAFDFH RLGQLRRLDL QYNQIRSLHP KTFEKLSRLE ELYLGNNLLQ
ALVPGTLAPL RKLRILYANG NEIGRLSRGS FEGLESLVKL RLDGNVLGAL PDAVFAPLGN
LLYLHLESNR IRFLGKNAFS QLGKLRFLNL SANELQPSLR HAATFVPLRS LSTLILSANS
LQHLGPRVFQ HLPRLGLLSL SGNQLTHLAP EAFWGLEALR ELRLEGNRLN QLPLTLLEPL
HSLEALDLSG NELSALHPAT FGHQGRLREL SLRDNALSAL SGDIFAASPA LYRLDLDGNG
WTCDCRLRGL KRWMGNWHSQ GRLLTVFVQC RHPPALRGKY LDYLDDQLLQ NGSCVDPSPS
PTAGSRQWPL PTSSEEGMTP PAGLSQELPL QPQPQPQQRG RLLPGVAWGG AAKELVGNRS
ALRLSRRGPG PHQGPSAAAP GSAPQSLDLH EKPGRGRHTR ANLSQTEPTP TSEPASGTPS
ARDSWQRAAK QRLASEQQES AVQSVSGVGL PPLVSDPCDF NKFILCNLTV EAVSANSASV
RWAVREHRSP RPQGGARFRL LFDRFGQQPK FQRFVYLPER SDSATLHELR GDTPYLVCVE
GVLGGRVCPV APRDHCAGLV TLPEAGGRGG VDYQLLTLVL LAVNALLVLL ALAAWGSRWL
RRKLRARRKG GAPVHVRHMY STRRPLRSMG TGVSADFSGF QSHRPRTTVC ALSEADLIEF
PCDRFMDSTG GGTSGSLRRE DHLLQRFAD
