TRIM1_HUMAN
ID TRIM1_HUMAN Reviewed; 735 AA.
AC Q9UJV3; A6NEL8; A6PVI5; Q5JYF5; Q8WWK1; Q9UJR9;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 3.
DT 03-AUG-2022, entry version 204.
DE RecName: Full=Probable E3 ubiquitin-protein ligase MID2;
DE EC=2.3.2.27;
DE AltName: Full=Midin-2;
DE AltName: Full=Midline defect 2;
DE AltName: Full=Midline-2;
DE AltName: Full=RING finger protein 60;
DE AltName: Full=RING-type E3 ubiquitin transferase MID2 {ECO:0000305};
DE AltName: Full=Tripartite motif-containing protein 1;
GN Name=MID2; Synonyms=FXY2, RNF60, TRIM1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=10644436; DOI=10.1006/geno.1999.6043;
RA Perry J., Short K.M., Romer J.T., Swift S., Cox T.C., Ashworth A.;
RT "FXY2/MID2, a gene related to the X-linked Opitz syndrome gene FXY/MID1,
RT maps to Xq22 and encodes a FNIII domain-containing protein that associates
RT with microtubules.";
RL Genomics 62:385-394(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Fetal brain;
RX PubMed=10400986; DOI=10.1093/hmg/8.8.1397;
RA Buchner G., Montini E., Andolfi G., Quaderi N., Cainarca S., Messali S.,
RA Bassi M.T., Ballabio A., Meroni G., Franco B.;
RT "MID2, a homologue of the Opitz syndrome gene MID1: similarities in a sub-
RT cellular localization and differences in expression during development.";
RL Hum. Mol. Genet. 8:1397-1407(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 12-735 (ISOFORM 2).
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 21-735 (ISOFORM 2).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INTERACTION WITH IGBP1, AND PHOSPHORYLATION.
RX PubMed=11806752; DOI=10.1186/1471-2121-3-1;
RA Short K.M., Hopwood B., Yi Z., Cox T.C.;
RT "MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive
RT PP2A regulatory subunit, Alpha 4, to microtubules: implications for the
RT clinical variability of X-linked Opitz GBBB syndrome and other
RT developmental disorders.";
RL BMC Cell Biol. 3:1-1(2002).
RN [7]
RP FUNCTION, INVOLVEMENT IN XLID101, VARIANT XLID101 GLN-347, VARIANT SER-343,
RP CHARACTERIZATION OF VARIANT XLID101 GLN-347, AND CHARACTERIZATION OF
RP VARIANT SER-343.
RX PubMed=24115387; DOI=10.1002/humu.22453;
RA Geetha T.S., Michealraj K.A., Kabra M., Kaur G., Juyal R.C., Thelma B.K.;
RT "Targeted deep resequencing identifies MID2 mutation for X-linked
RT intellectual disability with varied disease severity in a large kindred
RT from India.";
RL Hum. Mutat. 35:41-44(2014).
RN [8]
RP STRUCTURE BY NMR OF 394-537.
RG RIKEN structural genomics initiative (RSGI);
RT "The solution structure of the FN3 domain of human midline 2 protein.";
RL Submitted (OCT-2006) to the PDB data bank.
CC -!- FUNCTION: May play a role in microtubule stabilization.
CC {ECO:0000303|PubMed:24115387}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Homodimer or heterodimer with MID1. Interacts with IGBP1.
CC {ECO:0000269|PubMed:11806752}.
CC -!- INTERACTION:
CC Q9UJV3-2; Q6UY14-3: ADAMTSL4; NbExp=6; IntAct=EBI-10172526, EBI-10173507;
CC Q9UJV3-2; Q8WTP8: AEN; NbExp=3; IntAct=EBI-10172526, EBI-8637627;
CC Q9UJV3-2; P43353: ALDH3B1; NbExp=3; IntAct=EBI-10172526, EBI-2558314;
CC Q9UJV3-2; Q49AR9: ANKS1A; NbExp=3; IntAct=EBI-10172526, EBI-11954519;
CC Q9UJV3-2; Q9BXS5: AP1M1; NbExp=3; IntAct=EBI-10172526, EBI-541426;
CC Q9UJV3-2; P29972: AQP1; NbExp=3; IntAct=EBI-10172526, EBI-745213;
CC Q9UJV3-2; Q12774: ARHGEF5; NbExp=3; IntAct=EBI-10172526, EBI-602199;
CC Q9UJV3-2; Q8WXE1: ATRIP; NbExp=3; IntAct=EBI-10172526, EBI-747353;
CC Q9UJV3-2; A0A0S2Z4M1: AXIN1; NbExp=3; IntAct=EBI-10172526, EBI-16429430;
CC Q9UJV3-2; O15169: AXIN1; NbExp=3; IntAct=EBI-10172526, EBI-710484;
CC Q9UJV3-2; Q8TBE0: BAHD1; NbExp=3; IntAct=EBI-10172526, EBI-742750;
CC Q9UJV3-2; A8KA13: BCL6B; NbExp=6; IntAct=EBI-10172526, EBI-10174813;
CC Q9UJV3-2; P51451: BLK; NbExp=3; IntAct=EBI-10172526, EBI-2105445;
CC Q9UJV3-2; Q9H2G9: BLZF1; NbExp=5; IntAct=EBI-10172526, EBI-2548012;
CC Q9UJV3-2; Q5PSV4: BRMS1L; NbExp=3; IntAct=EBI-10172526, EBI-5666615;
CC Q9UJV3-2; Q13895: BYSL; NbExp=8; IntAct=EBI-10172526, EBI-358049;
CC Q9UJV3-2; Q13137: CALCOCO2; NbExp=3; IntAct=EBI-10172526, EBI-739580;
CC Q9UJV3-2; Q9H257-2: CARD9; NbExp=3; IntAct=EBI-10172526, EBI-11530605;
CC Q9UJV3-2; Q9Y2V2: CARHSP1; NbExp=3; IntAct=EBI-10172526, EBI-718719;
CC Q9UJV3-2; Q9HC52: CBX8; NbExp=6; IntAct=EBI-10172526, EBI-712912;
CC Q9UJV3-2; Q96HB5: CCDC120; NbExp=3; IntAct=EBI-10172526, EBI-744556;
CC Q9UJV3-2; Q96HB5-4: CCDC120; NbExp=3; IntAct=EBI-10172526, EBI-10185348;
CC Q9UJV3-2; Q8N715: CCDC185; NbExp=3; IntAct=EBI-10172526, EBI-740814;
CC Q9UJV3-2; Q96M95: CCDC42; NbExp=3; IntAct=EBI-10172526, EBI-747041;
CC Q9UJV3-2; Q96M95-2: CCDC42; NbExp=3; IntAct=EBI-10172526, EBI-11750401;
CC Q9UJV3-2; Q8TD31-3: CCHCR1; NbExp=6; IntAct=EBI-10172526, EBI-10175300;
CC Q9UJV3-2; Q07002: CDK18; NbExp=3; IntAct=EBI-10172526, EBI-746238;
CC Q9UJV3-2; Q8IYX8: CEP57L1; NbExp=3; IntAct=EBI-10172526, EBI-1104570;
CC Q9UJV3-2; Q8IYX8-2: CEP57L1; NbExp=3; IntAct=EBI-10172526, EBI-10181988;
CC Q9UJV3-2; P27918: CFP; NbExp=3; IntAct=EBI-10172526, EBI-9038570;
CC Q9UJV3-2; O14647: CHD2; NbExp=3; IntAct=EBI-10172526, EBI-1210503;
CC Q9UJV3-2; Q8NE01: CNNM3; NbExp=3; IntAct=EBI-10172526, EBI-741032;
CC Q9UJV3-2; P10606: COX5B; NbExp=3; IntAct=EBI-10172526, EBI-1053725;
CC Q9UJV3-2; O95639-2: CPSF4; NbExp=3; IntAct=EBI-10172526, EBI-13063650;
CC Q9UJV3-2; Q9BSW2: CRACR2A; NbExp=3; IntAct=EBI-10172526, EBI-739773;
CC Q9UJV3-2; Q8IUR6: CREBRF; NbExp=3; IntAct=EBI-10172526, EBI-1042699;
CC Q9UJV3-2; Q9UBR2: CTSZ; NbExp=6; IntAct=EBI-10172526, EBI-8636823;
CC Q9UJV3-2; O43602: DCX; NbExp=3; IntAct=EBI-10172526, EBI-8646694;
CC Q9UJV3-2; O43602-2: DCX; NbExp=3; IntAct=EBI-10172526, EBI-14148644;
CC Q9UJV3-2; Q9BY27: DGCR6L; NbExp=6; IntAct=EBI-10172526, EBI-742953;
CC Q9UJV3-2; Q9NQL9: DMRT3; NbExp=6; IntAct=EBI-10172526, EBI-9679045;
CC Q9UJV3-2; Q8WWB3: DYDC1; NbExp=3; IntAct=EBI-10172526, EBI-740680;
CC Q9UJV3-2; Q5JST6: EFHC2; NbExp=3; IntAct=EBI-10172526, EBI-2349927;
CC Q9UJV3-2; Q9UHF1: EGFL7; NbExp=3; IntAct=EBI-10172526, EBI-949532;
CC Q9UJV3-2; Q8N9N8: EIF1AD; NbExp=3; IntAct=EBI-10172526, EBI-750700;
CC Q9UJV3-2; Q14241: ELOA; NbExp=3; IntAct=EBI-10172526, EBI-742350;
CC Q9UJV3-2; Q9H0I2: ENKD1; NbExp=3; IntAct=EBI-10172526, EBI-744099;
CC Q9UJV3-2; O95208-2: EPN2; NbExp=3; IntAct=EBI-10172526, EBI-12135243;
CC Q9UJV3-2; Q9NVF9: ETNK2; NbExp=3; IntAct=EBI-10172526, EBI-751864;
CC Q9UJV3-2; O95990-3: FAM107A; NbExp=3; IntAct=EBI-10172526, EBI-10192902;
CC Q9UJV3-2; Q3B820: FAM161A; NbExp=6; IntAct=EBI-10172526, EBI-719941;
CC Q9UJV3-2; Q96MY7: FAM161B; NbExp=3; IntAct=EBI-10172526, EBI-7225287;
CC Q9UJV3-2; Q32MH5: FAM214A; NbExp=3; IntAct=EBI-10172526, EBI-2866142;
CC Q9UJV3-2; Q7L5A3: FAM214B; NbExp=3; IntAct=EBI-10172526, EBI-745689;
CC Q9UJV3-2; Q9Y247: FAM50B; NbExp=3; IntAct=EBI-10172526, EBI-742802;
CC Q9UJV3-2; Q86YD7: FAM90A1; NbExp=6; IntAct=EBI-10172526, EBI-6658203;
CC Q9UJV3-2; O95363: FARS2; NbExp=6; IntAct=EBI-10172526, EBI-2513774;
CC Q9UJV3-2; Q7L8L6: FASTKD5; NbExp=3; IntAct=EBI-10172526, EBI-747570;
CC Q9UJV3-2; Q8TES7-6: FBF1; NbExp=3; IntAct=EBI-10172526, EBI-10244131;
CC Q9UJV3-2; Q96D16: FBXL18; NbExp=3; IntAct=EBI-10172526, EBI-744419;
CC Q9UJV3-2; Q969U6: FBXW5; NbExp=3; IntAct=EBI-10172526, EBI-741068;
CC Q9UJV3-2; Q53SE7: FLJ13057; NbExp=3; IntAct=EBI-10172526, EBI-10172181;
CC Q9UJV3-2; Q6VB84: FOXD4L3; NbExp=3; IntAct=EBI-10172526, EBI-11961494;
CC Q9UJV3-2; Q96NE9: FRMD6; NbExp=3; IntAct=EBI-10172526, EBI-741729;
CC Q9UJV3-2; A1L4K1: FSD2; NbExp=3; IntAct=EBI-10172526, EBI-5661036;
CC Q9UJV3-2; Q8NHY3: GAS2L2; NbExp=3; IntAct=EBI-10172526, EBI-7960826;
CC Q9UJV3-2; O95995: GAS8; NbExp=3; IntAct=EBI-10172526, EBI-1052570;
CC Q9UJV3-2; Q96CN9: GCC1; NbExp=3; IntAct=EBI-10172526, EBI-746252;
CC Q9UJV3-2; P55040: GEM; NbExp=4; IntAct=EBI-10172526, EBI-744104;
CC Q9UJV3-2; Q96IK5: GMCL1; NbExp=5; IntAct=EBI-10172526, EBI-2548508;
CC Q9UJV3-2; Q08379: GOLGA2; NbExp=3; IntAct=EBI-10172526, EBI-618309;
CC Q9UJV3-2; Q7Z5G4: GOLGA7; NbExp=3; IntAct=EBI-10172526, EBI-4403685;
CC Q9UJV3-2; Q9H8Y8: GORASP2; NbExp=6; IntAct=EBI-10172526, EBI-739467;
CC Q9UJV3-2; O95872: GPANK1; NbExp=3; IntAct=EBI-10172526, EBI-751540;
CC Q9UJV3-2; Q92917: GPKOW; NbExp=3; IntAct=EBI-10172526, EBI-746309;
CC Q9UJV3-2; O15499: GSC2; NbExp=3; IntAct=EBI-10172526, EBI-19954058;
CC Q9UJV3-2; Q9GZV7: HAPLN2; NbExp=3; IntAct=EBI-10172526, EBI-11956675;
CC Q9UJV3-2; P08631-2: HCK; NbExp=3; IntAct=EBI-10172526, EBI-9834454;
CC Q9UJV3-2; P56524-2: HDAC4; NbExp=3; IntAct=EBI-10172526, EBI-11953488;
CC Q9UJV3-2; Q7Z353: HDX; NbExp=3; IntAct=EBI-10172526, EBI-1052734;
CC Q9UJV3-2; P14652: HOXB2; NbExp=3; IntAct=EBI-10172526, EBI-5329558;
CC Q9UJV3-2; P09067: HOXB5; NbExp=3; IntAct=EBI-10172526, EBI-3893317;
CC Q9UJV3-2; P17482: HOXB9; NbExp=6; IntAct=EBI-10172526, EBI-745290;
CC Q9UJV3-2; Q86VF2-5: IGFN1; NbExp=3; IntAct=EBI-10172526, EBI-11955401;
CC Q9UJV3-2; Q8IYA8: IHO1; NbExp=3; IntAct=EBI-10172526, EBI-8638439;
CC Q9UJV3-2; Q9UKT9: IKZF3; NbExp=5; IntAct=EBI-10172526, EBI-747204;
CC Q9UJV3-2; Q14005-2: IL16; NbExp=6; IntAct=EBI-10172526, EBI-17178971;
CC Q9UJV3-2; Q9C086: INO80B; NbExp=3; IntAct=EBI-10172526, EBI-715611;
CC Q9UJV3-2; Q9H0B3: IQCN; NbExp=3; IntAct=EBI-10172526, EBI-745878;
CC Q9UJV3-2; Q9H1K1: ISCU; NbExp=3; IntAct=EBI-10172526, EBI-1047335;
CC Q9UJV3-2; P16144-2: ITGB4; NbExp=3; IntAct=EBI-10172526, EBI-11051601;
CC Q9UJV3-2; Q15040: JOSD1; NbExp=3; IntAct=EBI-10172526, EBI-2510602;
CC Q9UJV3-2; Q63ZY3: KANK2; NbExp=3; IntAct=EBI-10172526, EBI-2556193;
CC Q9UJV3-2; Q9HAQ2: KIF9; NbExp=3; IntAct=EBI-10172526, EBI-8472129;
CC Q9UJV3-2; Q9BVG8: KIFC3; NbExp=3; IntAct=EBI-10172526, EBI-2125614;
CC Q9UJV3-2; Q9BVG8-5: KIFC3; NbExp=3; IntAct=EBI-10172526, EBI-14069005;
CC Q9UJV3-2; Q7Z3Y8: KRT27; NbExp=3; IntAct=EBI-10172526, EBI-3044087;
CC Q9UJV3-2; Q15323: KRT31; NbExp=3; IntAct=EBI-10172526, EBI-948001;
CC Q9UJV3-2; Q6A162: KRT40; NbExp=3; IntAct=EBI-10172526, EBI-10171697;
CC Q9UJV3-2; O95678: KRT75; NbExp=3; IntAct=EBI-10172526, EBI-2949715;
CC Q9UJV3-2; Q01546: KRT76; NbExp=3; IntAct=EBI-10172526, EBI-2952745;
CC Q9UJV3-2; Q14657: LAGE3; NbExp=3; IntAct=EBI-10172526, EBI-1052105;
CC Q9UJV3-2; Q96BZ8: LENG1; NbExp=6; IntAct=EBI-10172526, EBI-726510;
CC Q9UJV3-2; Q8TCE9: LGALS14; NbExp=6; IntAct=EBI-10172526, EBI-10274069;
CC Q9UJV3-2; O00214: LGALS8; NbExp=3; IntAct=EBI-10172526, EBI-740058;
CC Q9UJV3-2; O00214-2: LGALS8; NbExp=3; IntAct=EBI-10172526, EBI-12069522;
CC Q9UJV3-2; Q03252: LMNB2; NbExp=3; IntAct=EBI-10172526, EBI-2830427;
CC Q9UJV3-2; Q8IXW0: LMNTD2; NbExp=3; IntAct=EBI-10172526, EBI-12028858;
CC Q9UJV3-2; Q8TAP4-4: LMO3; NbExp=3; IntAct=EBI-10172526, EBI-11742507;
CC Q9UJV3-2; P61968: LMO4; NbExp=3; IntAct=EBI-10172526, EBI-2798728;
CC Q9UJV3-2; Q8TBB1: LNX1; NbExp=6; IntAct=EBI-10172526, EBI-739832;
CC Q9UJV3-2; Q17RB8: LONRF1; NbExp=3; IntAct=EBI-10172526, EBI-2341787;
CC Q9UJV3-2; Q96CN5: LRRC45; NbExp=3; IntAct=EBI-10172526, EBI-2805176;
CC Q9UJV3-2; Q9Y250: LZTS1; NbExp=3; IntAct=EBI-10172526, EBI-1216080;
CC Q9UJV3-2; Q9BRK4: LZTS2; NbExp=3; IntAct=EBI-10172526, EBI-741037;
CC Q9UJV3-2; P43360: MAGEA6; NbExp=3; IntAct=EBI-10172526, EBI-1045155;
CC Q9UJV3-2; Q96A72: MAGOHB; NbExp=3; IntAct=EBI-10172526, EBI-746778;
CC Q9UJV3-2; P50221: MEOX1; NbExp=3; IntAct=EBI-10172526, EBI-2864512;
CC Q9UJV3-2; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-10172526, EBI-16439278;
CC Q9UJV3-2; Q9H7H0: METTL17; NbExp=6; IntAct=EBI-10172526, EBI-749353;
CC Q9UJV3-2; Q9H7H0-2: METTL17; NbExp=3; IntAct=EBI-10172526, EBI-11098807;
CC Q9UJV3-2; P55081: MFAP1; NbExp=6; IntAct=EBI-10172526, EBI-1048159;
CC Q9UJV3-2; Q8IVT4: MGC50722; NbExp=3; IntAct=EBI-10172526, EBI-14086479;
CC Q9UJV3-2; O15344: MID1; NbExp=9; IntAct=EBI-10172526, EBI-2340316;
CC Q9UJV3-2; Q9NPA3: MID1IP1; NbExp=3; IntAct=EBI-10172526, EBI-750096;
CC Q9UJV3-2; Q9UJV3-2: MID2; NbExp=4; IntAct=EBI-10172526, EBI-10172526;
CC Q9UJV3-2; Q9BU76: MMTAG2; NbExp=3; IntAct=EBI-10172526, EBI-742459;
CC Q9UJV3-2; P00540: MOS; NbExp=3; IntAct=EBI-10172526, EBI-1757866;
CC Q9UJV3-2; Q13772-3: NCOA4; NbExp=3; IntAct=EBI-10172526, EBI-12054609;
CC Q9UJV3-2; Q6NSM0: NR1D2; NbExp=3; IntAct=EBI-10172526, EBI-10250949;
CC Q9UJV3-2; Q6X4W1-2: NSMF; NbExp=3; IntAct=EBI-10172526, EBI-12028784;
CC Q9UJV3-2; Q9UBU9: NXF1; NbExp=3; IntAct=EBI-10172526, EBI-398874;
CC Q9UJV3-2; Q96DC9: OTUB2; NbExp=3; IntAct=EBI-10172526, EBI-746259;
CC Q9UJV3-2; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-10172526, EBI-79165;
CC Q9UJV3-2; Q16512: PKN1; NbExp=3; IntAct=EBI-10172526, EBI-602382;
CC Q9UJV3-2; Q8WUT1: POLDIP3; NbExp=3; IntAct=EBI-10172526, EBI-10276663;
CC Q9UJV3-2; Q6NYC8: PPP1R18; NbExp=6; IntAct=EBI-10172526, EBI-2557469;
CC Q9UJV3-2; O43395: PRPF3; NbExp=3; IntAct=EBI-10172526, EBI-744322;
CC Q9UJV3-2; Q8WWY3: PRPF31; NbExp=11; IntAct=EBI-10172526, EBI-1567797;
CC Q9UJV3-2; P25786: PSMA1; NbExp=6; IntAct=EBI-10172526, EBI-359352;
CC Q9UJV3-2; Q8WV60: PTCD2; NbExp=3; IntAct=EBI-10172526, EBI-12154567;
CC Q9UJV3-2; A2A3K4: PTPDC1; NbExp=3; IntAct=EBI-10172526, EBI-11603375;
CC Q9UJV3-2; P47897: QARS1; NbExp=3; IntAct=EBI-10172526, EBI-347462;
CC Q9UJV3-2; Q9UJ41-4: RABGEF1; NbExp=3; IntAct=EBI-10172526, EBI-14093916;
CC Q9UJV3-2; Q86VV4: RANBP3L; NbExp=3; IntAct=EBI-10172526, EBI-12028066;
CC Q9UJV3-2; Q9P2K3: RCOR3; NbExp=3; IntAct=EBI-10172526, EBI-743428;
CC Q9UJV3-2; Q9P2K3-2: RCOR3; NbExp=3; IntAct=EBI-10172526, EBI-1504830;
CC Q9UJV3-2; Q04864-2: REL; NbExp=3; IntAct=EBI-10172526, EBI-10829018;
CC Q9UJV3-2; P04808: RLN1; NbExp=3; IntAct=EBI-10172526, EBI-12071382;
CC Q9UJV3-2; A0A0S2Z4G9: RNF6; NbExp=3; IntAct=EBI-10172526, EBI-16428950;
CC Q9UJV3-2; Q92753-1: RORB; NbExp=3; IntAct=EBI-10172526, EBI-18560266;
CC Q9UJV3-2; Q9UNE2: RPH3AL; NbExp=3; IntAct=EBI-10172526, EBI-2855824;
CC Q9UJV3-2; Q8N5L8: RPP25L; NbExp=3; IntAct=EBI-10172526, EBI-10189722;
CC Q9UJV3-2; Q06455-2: RUNX1T1; NbExp=3; IntAct=EBI-10172526, EBI-11984663;
CC Q9UJV3-2; Q06455-4: RUNX1T1; NbExp=3; IntAct=EBI-10172526, EBI-10224192;
CC Q9UJV3-2; P28702-3: RXRB; NbExp=3; IntAct=EBI-10172526, EBI-16429492;
CC Q9UJV3-2; Q9BWG6: SCNM1; NbExp=3; IntAct=EBI-10172526, EBI-748391;
CC Q9UJV3-2; O00560: SDCBP; NbExp=3; IntAct=EBI-10172526, EBI-727004;
CC Q9UJV3-2; Q8WYJ6: SEPTIN1; NbExp=3; IntAct=EBI-10172526, EBI-693002;
CC Q9UJV3-2; Q9NUL5: SHFL; NbExp=3; IntAct=EBI-10172526, EBI-10313866;
CC Q9UJV3-2; Q6ZT89: SLC25A48; NbExp=3; IntAct=EBI-10172526, EBI-10255185;
CC Q9UJV3-2; Q6ZT89-3: SLC25A48; NbExp=3; IntAct=EBI-10172526, EBI-12065614;
CC Q9UJV3-2; P12236: SLC25A6; NbExp=3; IntAct=EBI-10172526, EBI-356254;
CC Q9UJV3-2; O95863: SNAI1; NbExp=3; IntAct=EBI-10172526, EBI-1045459;
CC Q9UJV3-2; Q5SQN1: SNAP47; NbExp=3; IntAct=EBI-10172526, EBI-10244848;
CC Q9UJV3-2; Q86W54: SPATA24; NbExp=3; IntAct=EBI-10172526, EBI-3916986;
CC Q9UJV3-2; Q9NZD8: SPG21; NbExp=6; IntAct=EBI-10172526, EBI-742688;
CC Q9UJV3-2; O43597: SPRY2; NbExp=3; IntAct=EBI-10172526, EBI-742487;
CC Q9UJV3-2; O75558: STX11; NbExp=3; IntAct=EBI-10172526, EBI-714135;
CC Q9UJV3-2; Q96PV0: SYNGAP1; NbExp=3; IntAct=EBI-10172526, EBI-2682386;
CC Q9UJV3-2; Q9BSW7: SYT17; NbExp=10; IntAct=EBI-10172526, EBI-745392;
CC Q9UJV3-2; Q5VWN6: TASOR2; NbExp=3; IntAct=EBI-10172526, EBI-745958;
CC Q9UJV3-2; Q9NU19: TBC1D22B; NbExp=3; IntAct=EBI-10172526, EBI-8787464;
CC Q9UJV3-2; Q9Y4C2-2: TCAF1; NbExp=3; IntAct=EBI-10172526, EBI-11974855;
CC Q9UJV3-2; Q15560: TCEA2; NbExp=3; IntAct=EBI-10172526, EBI-710310;
CC Q9UJV3-2; Q8N8B7-2: TCEANC; NbExp=3; IntAct=EBI-10172526, EBI-11955057;
CC Q9UJV3-2; Q969V4: TEKT1; NbExp=3; IntAct=EBI-10172526, EBI-10180409;
CC Q9UJV3-2; Q9BT49: THAP7; NbExp=6; IntAct=EBI-10172526, EBI-741350;
CC Q9UJV3-2; O95985: TOP3B; NbExp=6; IntAct=EBI-10172526, EBI-373403;
CC Q9UJV3-2; P14373: TRIM27; NbExp=3; IntAct=EBI-10172526, EBI-719493;
CC Q9UJV3-2; Q8WV44: TRIM41; NbExp=3; IntAct=EBI-10172526, EBI-725997;
CC Q9UJV3-2; Q8IWZ5: TRIM42; NbExp=3; IntAct=EBI-10172526, EBI-5235829;
CC Q9UJV3-2; Q9BYV2: TRIM54; NbExp=6; IntAct=EBI-10172526, EBI-2130429;
CC Q9UJV3-2; Q86WT6-2: TRIM69; NbExp=3; IntAct=EBI-10172526, EBI-11525489;
CC Q9UJV3-2; Q9Y260: TRPV6; NbExp=3; IntAct=EBI-10172526, EBI-750052;
CC Q9UJV3-2; Q9BZW7: TSGA10; NbExp=3; IntAct=EBI-10172526, EBI-744794;
CC Q9UJV3-2; Q5W5X9-3: TTC23; NbExp=3; IntAct=EBI-10172526, EBI-9090990;
CC Q9UJV3-2; P51668: UBE2D1; NbExp=6; IntAct=EBI-10172526, EBI-743540;
CC Q9UJV3-2; P62837: UBE2D2; NbExp=3; IntAct=EBI-10172526, EBI-347677;
CC Q9UJV3-2; P61077: UBE2D3; NbExp=3; IntAct=EBI-10172526, EBI-348268;
CC Q9UJV3-2; Q9Y2X8: UBE2D4; NbExp=6; IntAct=EBI-10172526, EBI-745527;
CC Q9UJV3-2; Q96LR5: UBE2E2; NbExp=6; IntAct=EBI-10172526, EBI-2129763;
CC Q9UJV3-2; Q969T4: UBE2E3; NbExp=3; IntAct=EBI-10172526, EBI-348496;
CC Q9UJV3-2; P61086: UBE2K; NbExp=3; IntAct=EBI-10172526, EBI-473850;
CC Q9UJV3-2; Q9HAC8: UBTD1; NbExp=3; IntAct=EBI-10172526, EBI-745871;
CC Q9UJV3-2; Q9H3U1: UNC45A; NbExp=3; IntAct=EBI-10172526, EBI-1048763;
CC Q9UJV3-2; O75604: USP2; NbExp=3; IntAct=EBI-10172526, EBI-743272;
CC Q9UJV3-2; Q9UHP3: USP25; NbExp=3; IntAct=EBI-10172526, EBI-2513462;
CC Q9UJV3-2; Q70EL1-9: USP54; NbExp=3; IntAct=EBI-10172526, EBI-11975223;
CC Q9UJV3-2; Q9BRU9: UTP23; NbExp=3; IntAct=EBI-10172526, EBI-5457544;
CC Q9UJV3-2; Q68CQ4: UTP25; NbExp=3; IntAct=EBI-10172526, EBI-747711;
CC Q9UJV3-2; Q06250: WT1-AS; NbExp=3; IntAct=EBI-10172526, EBI-10223946;
CC Q9UJV3-2; Q05516: ZBTB16; NbExp=3; IntAct=EBI-10172526, EBI-711925;
CC Q9UJV3-2; O43167: ZBTB24; NbExp=6; IntAct=EBI-10172526, EBI-744471;
CC Q9UJV3-2; B2RXF5: ZBTB42; NbExp=3; IntAct=EBI-10172526, EBI-12287587;
CC Q9UJV3-2; Q53FD0: ZC2HC1C; NbExp=3; IntAct=EBI-10172526, EBI-740767;
CC Q9UJV3-2; Q9BQ24: ZFYVE21; NbExp=3; IntAct=EBI-10172526, EBI-2849569;
CC Q9UJV3-2; A0A0S2Z6H0: ZGPAT; NbExp=3; IntAct=EBI-10172526, EBI-16428984;
CC Q9UJV3-2; Q8N5A5: ZGPAT; NbExp=3; IntAct=EBI-10172526, EBI-3439227;
CC Q9UJV3-2; Q8N5A5-2: ZGPAT; NbExp=9; IntAct=EBI-10172526, EBI-10183064;
CC Q9UJV3-2; Q9NZV7: ZIM2; NbExp=3; IntAct=EBI-10172526, EBI-11962760;
CC Q9UJV3-2; Q15973: ZNF124; NbExp=3; IntAct=EBI-10172526, EBI-2555767;
CC Q9UJV3-2; P49910: ZNF165; NbExp=3; IntAct=EBI-10172526, EBI-741694;
CC Q9UJV3-2; Q53Z40: ZNF165; NbExp=3; IntAct=EBI-10172526, EBI-10186058;
CC Q9UJV3-2; Q13360-2: ZNF177; NbExp=3; IntAct=EBI-10172526, EBI-12272076;
CC Q9UJV3-2; P17028: ZNF24; NbExp=3; IntAct=EBI-10172526, EBI-707773;
CC Q9UJV3-2; P15622-3: ZNF250; NbExp=3; IntAct=EBI-10172526, EBI-10177272;
CC Q9UJV3-2; Q14584: ZNF266; NbExp=3; IntAct=EBI-10172526, EBI-7115319;
CC Q9UJV3-2; Q9H9D4: ZNF408; NbExp=3; IntAct=EBI-10172526, EBI-347633;
CC Q9UJV3-2; Q86VK4-3: ZNF410; NbExp=3; IntAct=EBI-10172526, EBI-11741890;
CC Q9UJV3-2; Q8TAU3: ZNF417; NbExp=6; IntAct=EBI-10172526, EBI-740727;
CC Q9UJV3-2; Q7Z4V0: ZNF438; NbExp=6; IntAct=EBI-10172526, EBI-11962468;
CC Q9UJV3-2; Q8IYI8: ZNF440; NbExp=6; IntAct=EBI-10172526, EBI-726439;
CC Q9UJV3-2; Q6ZNH5: ZNF497; NbExp=3; IntAct=EBI-10172526, EBI-10486136;
CC Q9UJV3-2; Q9H707: ZNF552; NbExp=3; IntAct=EBI-10172526, EBI-2555731;
CC Q9UJV3-2; Q8TBZ8: ZNF564; NbExp=6; IntAct=EBI-10172526, EBI-10273713;
CC Q9UJV3-2; Q96SQ5: ZNF587; NbExp=6; IntAct=EBI-10172526, EBI-6427977;
CC Q9UJV3-2; Q5T619: ZNF648; NbExp=3; IntAct=EBI-10172526, EBI-11985915;
CC Q9UJV3-2; Q96BR6: ZNF669; NbExp=3; IntAct=EBI-10172526, EBI-12006574;
CC Q9UJV3-2; A0A0S2Z5X4: ZNF688; NbExp=3; IntAct=EBI-10172526, EBI-16429014;
CC Q9UJV3-2; Q9H7X3: ZNF696; NbExp=3; IntAct=EBI-10172526, EBI-11090299;
CC Q9UJV3-2; Q96H86: ZNF764; NbExp=3; IntAct=EBI-10172526, EBI-745775;
CC Q9UJV3-2; Q6NX45: ZNF774; NbExp=3; IntAct=EBI-10172526, EBI-10251462;
CC Q9UJV3-2; Q8NCA9: ZNF784; NbExp=3; IntAct=EBI-10172526, EBI-7138303;
CC Q9UJV3-2; A8K8V0: ZNF785; NbExp=3; IntAct=EBI-10172526, EBI-3925400;
CC Q9UJV3-2; Q3KQV3: ZNF792; NbExp=6; IntAct=EBI-10172526, EBI-10240849;
CC Q9UJV3-2; Q9UGI0: ZRANB1; NbExp=3; IntAct=EBI-10172526, EBI-527853;
CC Q9UJV3-2; O43309: ZSCAN12; NbExp=6; IntAct=EBI-10172526, EBI-1210440;
CC Q9UJV3-2; B3KPU6; NbExp=3; IntAct=EBI-10172526, EBI-10175879;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton.
CC Note=Microtubule-associated.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9UJV3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9UJV3-2; Sequence=VSP_009009;
CC -!- TISSUE SPECIFICITY: Low level in fetal kidney and lung, and in adult
CC prostate, ovary and small intestine.
CC -!- DOMAIN: The tripartite motif (RBCC; RING- and B box-type zinc fingers
CC and coiled coil domains) mediates dimerization. {ECO:0000250}.
CC -!- DOMAIN: Associates with microtubules in a manner that is dependent on
CC the C-terminal B30.2 domain.
CC -!- PTM: Phosphorylated on serine and threonine residues.
CC {ECO:0000269|PubMed:11806752}.
CC -!- DISEASE: Intellectual developmental disorder, X-linked 101 (XLID101)
CC [MIM:300928]: A disorder characterized by significantly below average
CC general intellectual functioning associated with impairments in
CC adaptive behavior and manifested during the developmental period.
CC Intellectual deficiency is the only primary symptom of non-syndromic X-
CC linked forms, while syndromic forms present with associated physical,
CC neurological and/or psychiatric manifestations. XLID101 clinical
CC features include global developmental delay, hyperactivity often with
CC aggressive outbursts, and seizures in some patients. Several affected
CC individuals have long face, prominent ears, and squint or strabismus.
CC {ECO:0000269|PubMed:24115387}. Note=The disease is caused by variants
CC affecting the gene represented in this entry.
CC -!- SIMILARITY: Belongs to the TRIM/RBCC family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-21 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF07341.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH17707.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB56154.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF196481; AAF07341.1; ALT_INIT; mRNA.
DR EMBL; Y18880; CAB56154.1; ALT_INIT; mRNA.
DR EMBL; AL034399; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL109946; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC017707; AAH17707.1; ALT_INIT; mRNA.
DR EMBL; BT006663; AAP35309.1; -; mRNA.
DR CCDS; CCDS14532.2; -. [Q9UJV3-1]
DR CCDS; CCDS14533.2; -. [Q9UJV3-2]
DR RefSeq; NP_036348.2; NM_012216.3. [Q9UJV3-1]
DR RefSeq; NP_438112.2; NM_052817.2. [Q9UJV3-2]
DR RefSeq; XP_005262119.1; XM_005262062.4.
DR RefSeq; XP_016884728.1; XM_017029239.1.
DR PDB; 2DJA; NMR; -; A=182-252.
DR PDB; 2DMK; NMR; -; A=394-537.
DR PDBsum; 2DJA; -.
DR PDBsum; 2DMK; -.
DR AlphaFoldDB; Q9UJV3; -.
DR BMRB; Q9UJV3; -.
DR SMR; Q9UJV3; -.
DR BioGRID; 116231; 279.
DR IntAct; Q9UJV3; 252.
DR MINT; Q9UJV3; -.
DR STRING; 9606.ENSP00000262843; -.
DR MoonDB; Q9UJV3; Predicted.
DR iPTMnet; Q9UJV3; -.
DR PhosphoSitePlus; Q9UJV3; -.
DR BioMuta; MID2; -.
DR DMDM; 294862489; -.
DR EPD; Q9UJV3; -.
DR jPOST; Q9UJV3; -.
DR MassIVE; Q9UJV3; -.
DR MaxQB; Q9UJV3; -.
DR PaxDb; Q9UJV3; -.
DR PeptideAtlas; Q9UJV3; -.
DR PRIDE; Q9UJV3; -.
DR ProteomicsDB; 84662; -. [Q9UJV3-1]
DR ProteomicsDB; 84663; -. [Q9UJV3-2]
DR Antibodypedia; 29334; 188 antibodies from 27 providers.
DR DNASU; 11043; -.
DR Ensembl; ENST00000262843.11; ENSP00000262843.6; ENSG00000080561.14. [Q9UJV3-1]
DR Ensembl; ENST00000443968.2; ENSP00000413976.2; ENSG00000080561.14. [Q9UJV3-2]
DR GeneID; 11043; -.
DR KEGG; hsa:11043; -.
DR MANE-Select; ENST00000262843.11; ENSP00000262843.6; NM_012216.4; NP_036348.2.
DR UCSC; uc004enk.4; human. [Q9UJV3-1]
DR CTD; 11043; -.
DR DisGeNET; 11043; -.
DR GeneCards; MID2; -.
DR HGNC; HGNC:7096; MID2.
DR HPA; ENSG00000080561; Low tissue specificity.
DR MalaCards; MID2; -.
DR MIM; 300204; gene.
DR MIM; 300928; phenotype.
DR neXtProt; NX_Q9UJV3; -.
DR OpenTargets; ENSG00000080561; -.
DR Orphanet; 777; X-linked non-syndromic intellectual disability.
DR PharmGKB; PA30817; -.
DR VEuPathDB; HostDB:ENSG00000080561; -.
DR eggNOG; KOG2177; Eukaryota.
DR GeneTree; ENSGT00940000159460; -.
DR HOGENOM; CLU_013137_19_4_1; -.
DR InParanoid; Q9UJV3; -.
DR OMA; GAPRGKC; -.
DR OrthoDB; 172471at2759; -.
DR PhylomeDB; Q9UJV3; -.
DR TreeFam; TF333654; -.
DR PathwayCommons; Q9UJV3; -.
DR SignaLink; Q9UJV3; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 11043; 8 hits in 737 CRISPR screens.
DR ChiTaRS; MID2; human.
DR EvolutionaryTrace; Q9UJV3; -.
DR GeneWiki; MID2; -.
DR GenomeRNAi; 11043; -.
DR Pharos; Q9UJV3; Tbio.
DR PRO; PR:Q9UJV3; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q9UJV3; protein.
DR Bgee; ENSG00000080561; Expressed in tibialis anterior and 177 other tissues.
DR ExpressionAtlas; Q9UJV3; baseline and differential.
DR Genevisible; Q9UJV3; HS.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005874; C:microtubule; IDA:UniProtKB.
DR GO; GO:0019899; F:enzyme binding; IPI:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0008017; F:microtubule binding; IMP:UniProtKB.
DR GO; GO:0051219; F:phosphoprotein binding; IPI:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:ARUK-UCL.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045087; P:innate immune response; IDA:UniProtKB.
DR GO; GO:0046597; P:negative regulation of viral entry into host cell; IDA:UniProtKB.
DR GO; GO:0032897; P:negative regulation of viral transcription; IDA:UniProtKB.
DR GO; GO:0010508; P:positive regulation of autophagy; IMP:UniProtKB.
DR GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; IDA:UniProtKB.
DR GO; GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; IMP:UniProtKB.
DR GO; GO:0035372; P:protein localization to microtubule; IMP:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0044790; P:suppression of viral release by host; IDA:UniProtKB.
DR CDD; cd00063; FN3; 1.
DR CDD; cd13739; SPRY_PRY_TRIM1; 1.
DR Gene3D; 2.60.120.920; -; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR003649; Bbox_C.
DR InterPro; IPR003879; Butyrophylin_SPRY.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR017903; COS_domain.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR033491; MID2.
DR InterPro; IPR040859; Midline-1_COS.
DR InterPro; IPR035752; SPRY/PRY_TRIM1.
DR InterPro; IPR003877; SPRY_dom.
DR InterPro; IPR027370; Znf-RING_LisH.
DR InterPro; IPR000315; Znf_B-box.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR24099:SF12; PTHR24099:SF12; 1.
DR Pfam; PF18568; COS; 1.
DR Pfam; PF00622; SPRY; 1.
DR Pfam; PF00643; zf-B_box; 1.
DR Pfam; PF13445; zf-RING_UBOX; 1.
DR PRINTS; PR01407; BUTYPHLNCDUF.
DR SMART; SM00502; BBC; 1.
DR SMART; SM00336; BBOX; 2.
DR SMART; SM00060; FN3; 1.
DR SMART; SM00184; RING; 1.
DR SMART; SM00449; SPRY; 1.
DR SUPFAM; SSF49265; SSF49265; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS50188; B302_SPRY; 1.
DR PROSITE; PS51262; COS; 1.
DR PROSITE; PS50119; ZF_BBOX; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton;
KW Disease variant; Intellectual disability; Metal-binding; Microtubule;
KW Phosphoprotein; Reference proteome; Repeat; Transferase;
KW Ubl conjugation pathway; Zinc; Zinc-finger.
FT CHAIN 1..735
FT /note="Probable E3 ubiquitin-protein ligase MID2"
FT /id="PRO_0000056193"
FT DOMAIN 340..399
FT /note="COS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00586"
FT DOMAIN 398..531
FT /note="Fibronectin type-III"
FT DOMAIN 516..709
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT ZN_FING 30..80
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT ZN_FING 137..184
FT /note="B box-type 1; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT ZN_FING 190..232
FT /note="B box-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT COILED 233..301
FT /evidence="ECO:0000255"
FT BINDING 195
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 198
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 218
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT VAR_SEQ 450..479
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10644436,
FT ECO:0000303|PubMed:15489334, ECO:0000303|Ref.5"
FT /id="VSP_009009"
FT VARIANT 343
FT /note="N -> S (the protein is normally bound to
FT microtubules; dbSNP:rs551253128)"
FT /evidence="ECO:0000269|PubMed:24115387"
FT /id="VAR_071835"
FT VARIANT 347
FT /note="R -> Q (in XLID101; the mutant is abnormally
FT localized in aggregates or enclosed in cytoplasmic vesicles
FT rather than being bound to microtubules;
FT dbSNP:rs587777605)"
FT /evidence="ECO:0000269|PubMed:24115387"
FT /id="VAR_071836"
FT VARIANT 378
FT /note="A -> D (in dbSNP:rs12849510)"
FT /id="VAR_052123"
FT STRAND 199..201
FT /evidence="ECO:0007829|PDB:2DJA"
FT STRAND 205..207
FT /evidence="ECO:0007829|PDB:2DJA"
FT TURN 208..211
FT /evidence="ECO:0007829|PDB:2DJA"
FT STRAND 212..214
FT /evidence="ECO:0007829|PDB:2DJA"
FT HELIX 216..220
FT /evidence="ECO:0007829|PDB:2DJA"
FT TURN 223..226
FT /evidence="ECO:0007829|PDB:2DJA"
FT STRAND 406..415
FT /evidence="ECO:0007829|PDB:2DMK"
FT STRAND 418..424
FT /evidence="ECO:0007829|PDB:2DMK"
FT STRAND 429..442
FT /evidence="ECO:0007829|PDB:2DMK"
FT HELIX 483..486
FT /evidence="ECO:0007829|PDB:2DMK"
FT STRAND 487..493
FT /evidence="ECO:0007829|PDB:2DMK"
FT STRAND 495..502
FT /evidence="ECO:0007829|PDB:2DMK"
FT STRAND 508..519
FT /evidence="ECO:0007829|PDB:2DMK"
FT STRAND 521..523
FT /evidence="ECO:0007829|PDB:2DMK"
FT STRAND 527..530
FT /evidence="ECO:0007829|PDB:2DMK"
SQ SEQUENCE 735 AA; 83210 MW; 22A0EC26B050EA21 CRC64;
MGESPASVVL NASGGLFSLK METLESELTC PICLELFEDP LLLPCAHSLC FSCAHRILVS
SCSSGESIEP ITAFQCPTCR YVISLNHRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSES
RRERTYRPTT AMSSERIACQ FCEQDPPRDA VKTCITCEVS YCDRCLRATH PNKKPFTSHR
LVEPVPDTHL RGITCLDHEN EKVNMYCVSD DQLICALCKL VGRHRDHQVA SLNDRFEKLK
QTLEMNLTNL VKRNSELENQ MAKLIQICQQ VEVNTAMHEA KLMEECDELV EIIQQRKQMI
AVKIKETKVM KLRKLAQQVA NCRQCLERST VLINQAEHIL KENDQARFLQ SAKNIAERVA
MATASSQVLI PDINFNDAFE NFALDFSREK KLLEGLDYLT APNPPSIREE LCTASHDTIT
VHWISDDEFS ISSYELQYTI FTGQANFISK SWCSWGLWPE IRKCKEAVSC SRLAGAPRGL
YNSVDSWMIV PNIKQNHYTV HGLQSGTRYI FIVKAINQAG SRNSEPTRLK TNSQPFKLDP
KMTHKKLKIS NDGLQMEKDE SSLKKSHTPE RFSGTGCYGA AGNIFIDSGC HYWEVVMGSS
TWYAIGIAYK SAPKNEWIGK NASSWVFSRC NSNFVVRHNN KEMLVDVPPH LKRLGVLLDY
DNNMLSFYDP ANSLHLHTFD VTFILPVCPT FTIWNKSLMI LSGLPAPDFI DYPERQECNC
RPQESPYVSG MKTCH
