TRIMM_HUMAN
ID TRIMM_HUMAN Reviewed; 437 AA.
AC Q8N7C3; B7Z6J6;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Probable E3 ubiquitin-protein ligase TRIML2 {ECO:0000305};
DE EC=2.3.2.27;
DE AltName: Full=RING-type E3 ubiquitin transferase TRIML2 {ECO:0000305};
DE AltName: Full=SPRY domain-containing protein 6;
DE AltName: Full=Tripartite motif family-like protein 2;
GN Name=TRIML2 {ECO:0000312|HGNC:HGNC:26378}; Synonyms=SPRYD6;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 39-437 (ISOFORM 1), AND
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Placenta, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 41-437 (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- INTERACTION:
CC Q8N7C3; Q9NYB9-2: ABI2; NbExp=3; IntAct=EBI-11059915, EBI-11096309;
CC Q8N7C3; Q6QNY1: BLOC1S2; NbExp=4; IntAct=EBI-11059915, EBI-465872;
CC Q8N7C3; Q8NA61-2: CBY2; NbExp=3; IntAct=EBI-11059915, EBI-11524851;
CC Q8N7C3; Q8IYE0: CCDC146; NbExp=3; IntAct=EBI-11059915, EBI-10749669;
CC Q8N7C3; Q53HC0: CCDC92; NbExp=6; IntAct=EBI-11059915, EBI-719994;
CC Q8N7C3; Q8TD31-3: CCHCR1; NbExp=3; IntAct=EBI-11059915, EBI-10175300;
CC Q8N7C3; P51946: CCNH; NbExp=3; IntAct=EBI-11059915, EBI-741406;
CC Q8N7C3; Q01850: CDR2; NbExp=3; IntAct=EBI-11059915, EBI-1181367;
CC Q8N7C3; P56545-3: CTBP2; NbExp=3; IntAct=EBI-11059915, EBI-10171902;
CC Q8N7C3; Q14232: EIF2B1; NbExp=3; IntAct=EBI-11059915, EBI-491065;
CC Q8N7C3; Q7L2H7: EIF3M; NbExp=3; IntAct=EBI-11059915, EBI-353901;
CC Q8N7C3; Q86YD7: FAM90A1; NbExp=3; IntAct=EBI-11059915, EBI-6658203;
CC Q8N7C3; Q8IZT9: FAM9C; NbExp=3; IntAct=EBI-11059915, EBI-2870039;
CC Q8N7C3; O14964: HGS; NbExp=3; IntAct=EBI-11059915, EBI-740220;
CC Q8N7C3; Q8WYH8: ING5; NbExp=3; IntAct=EBI-11059915, EBI-488533;
CC Q8N7C3; Q13352: ITGB3BP; NbExp=3; IntAct=EBI-11059915, EBI-712105;
CC Q8N7C3; P19012: KRT15; NbExp=3; IntAct=EBI-11059915, EBI-739566;
CC Q8N7C3; Q2M2I5: KRT24; NbExp=3; IntAct=EBI-11059915, EBI-2952736;
CC Q8N7C3; Q7Z3Y8: KRT27; NbExp=3; IntAct=EBI-11059915, EBI-3044087;
CC Q8N7C3; Q15323: KRT31; NbExp=3; IntAct=EBI-11059915, EBI-948001;
CC Q8N7C3; O76011: KRT34; NbExp=3; IntAct=EBI-11059915, EBI-1047093;
CC Q8N7C3; O76015: KRT38; NbExp=3; IntAct=EBI-11059915, EBI-1047263;
CC Q8N7C3; O95678: KRT75; NbExp=3; IntAct=EBI-11059915, EBI-2949715;
CC Q8N7C3; Q8NHZ7: MBD3L2; NbExp=3; IntAct=EBI-11059915, EBI-11989378;
CC Q8N7C3; A6NE82: MBD3L3; NbExp=3; IntAct=EBI-11059915, EBI-17422057;
CC Q8N7C3; P50221: MEOX1; NbExp=3; IntAct=EBI-11059915, EBI-2864512;
CC Q8N7C3; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-11059915, EBI-16439278;
CC Q8N7C3; Q7Z6G3-2: NECAB2; NbExp=4; IntAct=EBI-11059915, EBI-10172876;
CC Q8N7C3; Q8NI38: NFKBID; NbExp=3; IntAct=EBI-11059915, EBI-10271199;
CC Q8N7C3; P37198: NUP62; NbExp=3; IntAct=EBI-11059915, EBI-347978;
CC Q8N7C3; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-11059915, EBI-79165;
CC Q8N7C3; Q9P1U0: POLR1H; NbExp=3; IntAct=EBI-11059915, EBI-355434;
CC Q8N7C3; P78424: POU6F2; NbExp=3; IntAct=EBI-11059915, EBI-12029004;
CC Q8N7C3; Q9H788: SH2D4A; NbExp=3; IntAct=EBI-11059915, EBI-747035;
CC Q8N7C3; Q9H668: STN1; NbExp=3; IntAct=EBI-11059915, EBI-746930;
CC Q8N7C3; Q6PIF2: SYCE2; NbExp=5; IntAct=EBI-11059915, EBI-11958386;
CC Q8N7C3; Q3MII6: TBC1D25; NbExp=3; IntAct=EBI-11059915, EBI-11899977;
CC Q8N7C3; P15884-3: TCF4; NbExp=3; IntAct=EBI-11059915, EBI-13636688;
CC Q8N7C3; Q9UBB9: TFIP11; NbExp=3; IntAct=EBI-11059915, EBI-1105213;
CC Q8N7C3; Q9BYV2: TRIM54; NbExp=3; IntAct=EBI-11059915, EBI-2130429;
CC Q8N7C3; Q8N7C3: TRIML2; NbExp=3; IntAct=EBI-11059915, EBI-11059915;
CC Q8N7C3; Q7Z6J9: TSEN54; NbExp=3; IntAct=EBI-11059915, EBI-2559824;
CC Q8N7C3; Q99816: TSG101; NbExp=3; IntAct=EBI-11059915, EBI-346882;
CC Q8N7C3; Q8N6Y0: USHBP1; NbExp=3; IntAct=EBI-11059915, EBI-739895;
CC Q8N7C3; Q6GPH4: XAF1; NbExp=3; IntAct=EBI-11059915, EBI-2815120;
CC Q8N7C3; O96006: ZBED1; NbExp=3; IntAct=EBI-11059915, EBI-740037;
CC Q8N7C3; Q86T24: ZBTB33; NbExp=5; IntAct=EBI-11059915, EBI-2515625;
CC Q8N7C3; Q15911-2: ZFHX3; NbExp=3; IntAct=EBI-11059915, EBI-10237226;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N7C3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N7C3-2; Sequence=VSP_055447, VSP_055448;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI11960.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAI11962.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC05372.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC108073; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK098667; BAC05372.1; ALT_INIT; mRNA.
DR EMBL; AK300428; BAH13282.1; -; mRNA.
DR EMBL; BC111959; AAI11960.1; ALT_INIT; mRNA.
DR EMBL; BC111961; AAI11962.1; ALT_INIT; mRNA.
DR CCDS; CCDS3850.2; -. [Q8N7C3-1]
DR RefSeq; NP_001290348.1; NM_001303419.1.
DR RefSeq; NP_775824.2; NM_173553.2.
DR AlphaFoldDB; Q8N7C3; -.
DR SMR; Q8N7C3; -.
DR BioGRID; 128500; 87.
DR IntAct; Q8N7C3; 58.
DR STRING; 9606.ENSP00000422581; -.
DR iPTMnet; Q8N7C3; -.
DR PhosphoSitePlus; Q8N7C3; -.
DR BioMuta; TRIML2; -.
DR DMDM; 74714998; -.
DR EPD; Q8N7C3; -.
DR jPOST; Q8N7C3; -.
DR MassIVE; Q8N7C3; -.
DR MaxQB; Q8N7C3; -.
DR PaxDb; Q8N7C3; -.
DR PeptideAtlas; Q8N7C3; -.
DR PRIDE; Q8N7C3; -.
DR ProteomicsDB; 6780; -.
DR ProteomicsDB; 72286; -. [Q8N7C3-1]
DR Antibodypedia; 49227; 52 antibodies from 14 providers.
DR DNASU; 205860; -.
DR Ensembl; ENST00000512729.5; ENSP00000422581.2; ENSG00000179046.9. [Q8N7C3-1]
DR Ensembl; ENST00000682553.1; ENSP00000507413.1; ENSG00000179046.9. [Q8N7C3-1]
DR GeneID; 205860; -.
DR KEGG; hsa:205860; -.
DR MANE-Select; ENST00000682553.1; ENSP00000507413.1; NM_173553.4; NP_775824.2.
DR UCSC; uc003izl.3; human. [Q8N7C3-1]
DR CTD; 205860; -.
DR DisGeNET; 205860; -.
DR GeneCards; TRIML2; -.
DR HGNC; HGNC:26378; TRIML2.
DR HPA; ENSG00000179046; Group enriched (placenta, testis).
DR neXtProt; NX_Q8N7C3; -.
DR OpenTargets; ENSG00000179046; -.
DR PharmGKB; PA162407031; -.
DR VEuPathDB; HostDB:ENSG00000179046; -.
DR eggNOG; KOG2177; Eukaryota.
DR GeneTree; ENSGT00940000162029; -.
DR InParanoid; Q8N7C3; -.
DR OrthoDB; 423686at2759; -.
DR PhylomeDB; Q8N7C3; -.
DR TreeFam; TF338674; -.
DR PathwayCommons; Q8N7C3; -.
DR SignaLink; Q8N7C3; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 205860; 7 hits in 1100 CRISPR screens.
DR GenomeRNAi; 205860; -.
DR Pharos; Q8N7C3; Tdark.
DR PRO; PR:Q8N7C3; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q8N7C3; protein.
DR Bgee; ENSG00000179046; Expressed in right testis and 86 other tissues.
DR ExpressionAtlas; Q8N7C3; baseline and differential.
DR Genevisible; Q8N7C3; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0042803; F:protein homodimerization activity; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR GO; GO:0010508; P:positive regulation of autophagy; IBA:GO_Central.
DR GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; IBA:GO_Central.
DR GO; GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR GO; GO:0032880; P:regulation of protein localization; IBA:GO_Central.
DR GO; GO:0046596; P:regulation of viral entry into host cell; IBA:GO_Central.
DR Gene3D; 2.60.120.920; -; 1.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR003879; Butyrophylin_SPRY.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR006574; PRY.
DR InterPro; IPR003877; SPRY_dom.
DR InterPro; IPR000315; Znf_B-box.
DR Pfam; PF13765; PRY; 1.
DR Pfam; PF00622; SPRY; 1.
DR Pfam; PF00643; zf-B_box; 1.
DR PRINTS; PR01407; BUTYPHLNCDUF.
DR SMART; SM00589; PRY; 1.
DR SMART; SM00449; SPRY; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS50188; B302_SPRY; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..437
FT /note="Probable E3 ubiquitin-protein ligase TRIML2"
FT /id="PRO_0000317435"
FT DOMAIN 231..429
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT COILED 55..200
FT /evidence="ECO:0000255"
FT VAR_SEQ 208..221
FT /note="NAKYSLERSKSLLL -> VRCWEKENKIHSSI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055447"
FT VAR_SEQ 222..437
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055448"
SQ SEQUENCE 437 AA; 49893 MW; BC2532BEF2CA551F CRC64;
MSKRLSPQLQ HNITEDAYCE THLEPTRLFC DVDQITLCSK CFQSQEHKHH MVCGIQEAAE
NYRKLFQEIL NTSREKLEAA KSILTDEQER MAMIQEEEQN FKKMIESEYS MRLRLLNEEC
EQNLQRQQEC ISDLNLRETL LNQAIKLATE LEEMFQEMLQ RLGRVGRENM EKLKESEARA
SEQVRSLLKL IVELEKKCGE GTLALLKNAK YSLERSKSLL LEHLEPAHIT DLSLCHIRGL
SSMFRVLQRH LTLDPETAHP CLALSEDLRT MRLRHGQQDG AGNPERLDFS AMVLAAESFT
SGRHYWEVDV EKATRWQVGI YHGSADAKGS TARASGEKVL LTGSVMGTEW TLWVFPPLKR
LFLEKKLDTV GVFLDCEHGQ ISFYNVTEMS LIYNFSHCAF QGALRPVFSL CIPNGDTSPD
SLTILQHGPS CDATVSP
