TRISS_DROME
ID TRISS_DROME Reviewed; 108 AA.
AC Q4V645; Q9VF57;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Trissin {ECO:0000303|PubMed:21939639};
DE Flags: Precursor;
GN Name=Trissin {ECO:0000303|PubMed:21939639};
GN ORFNames=CG14871 {ECO:0000312|FlyBase:FBgn0038343};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAY54877.1};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAY54877.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Celniker S.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP PROTEIN SEQUENCE OF 30-55, FUNCTION, MASS SPECTROMETRY, AND DISULFIDE
RP BONDS.
RX PubMed=21939639; DOI=10.1016/j.bbrc.2011.09.018;
RA Ida T., Takahashi T., Tominaga H., Sato T., Kume K., Yoshizawa-Kumagaye K.,
RA Nishio H., Kato J., Murakami N., Miyazato M., Kangawa K., Kojima M.;
RT "Identification of the endogenous cysteine-rich peptide trissin, a ligand
RT for an orphan G protein-coupled receptor in Drosophila.";
RL Biochem. Biophys. Res. Commun. 414:44-48(2011).
CC -!- FUNCTION: Activates the G-protein coupled receptor TrissinR in vitro,
CC leading to increased intracellular calcium ion levels.
CC {ECO:0000269|PubMed:21939639}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=2996.02; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:21939639};
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DR EMBL; AE014297; AAF55203.2; -; Genomic_DNA.
DR EMBL; BT022438; AAY54854.1; -; mRNA.
DR EMBL; BT022461; AAY54877.1; -; mRNA.
DR RefSeq; NP_650471.2; NM_142214.2.
DR AlphaFoldDB; Q4V645; -.
DR STRING; 7227.FBpp0289411; -.
DR PaxDb; Q4V645; -.
DR PRIDE; Q4V645; -.
DR DNASU; 41890; -.
DR EnsemblMetazoa; FBtr0300174; FBpp0289411; FBgn0038343.
DR GeneID; 41890; -.
DR KEGG; dme:Dmel_CG14871; -.
DR UCSC; CG14871-RB; d. melanogaster.
DR CTD; 41890; -.
DR FlyBase; FBgn0038343; Trissin.
DR VEuPathDB; VectorBase:FBgn0038343; -.
DR eggNOG; ENOG502SFTC; Eukaryota.
DR HOGENOM; CLU_175791_0_0_1; -.
DR InParanoid; Q4V645; -.
DR OMA; LRERRHN; -.
DR OrthoDB; 1560632at2759; -.
DR PhylomeDB; Q4V645; -.
DR BioGRID-ORCS; 41890; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41890; -.
DR PRO; PR:Q4V645; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038343; Expressed in brain and 6 other tissues.
DR ExpressionAtlas; Q4V645; baseline and differential.
DR GO; GO:0005615; C:extracellular space; ISM:FlyBase.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IPI:FlyBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:FlyBase.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000269|PubMed:21939639"
FT PEPTIDE 30..56
FT /note="Trissin"
FT /evidence="ECO:0000269|PubMed:21939639"
FT /id="PRO_0000437962"
FT PROPEP 57..108
FT /evidence="ECO:0000305|PubMed:21939639"
FT /id="PRO_0000437963"
FT DISULFID 32..43
FT /evidence="ECO:0000269|PubMed:21939639"
FT DISULFID 35..52
FT /evidence="ECO:0000269|PubMed:21939639"
FT DISULFID 39..51
FT /evidence="ECO:0000269|PubMed:21939639"
SQ SEQUENCE 108 AA; 12573 MW; AED22A91A7C37418 CRC64;
MTKTTMHWLA HFQIILLCIW LMCPPSSQAI KCDTCGKECA SACGTKHFRT CCFNYLRKRS
DPDALRQSSN RRLIDFILLQ GRALFTQELR ERRHNGTLMD LGLNTYYP
