TRKA_AZOC5
ID TRKA_AZOC5 Reviewed; 458 AA.
AC Q04856; A8IBK0;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Trk system potassium uptake protein TrkA;
DE Short=K(+)-uptake protein TrkA;
GN Name=trkA; OrderedLocusNames=AZC_3082;
OS Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS 6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Xanthobacteraceae; Azorhizobium.
OX NCBI_TaxID=438753;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB
RC 13405 / ORS 571;
RA Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT caulinodans ORS571.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111.
RX PubMed=1661370; DOI=10.1007/bf00293830;
RA Pawlowski K., Klosse U., de Bruijn F.J.;
RT "Characterization of a novel Azorhizobium caulinodans ORS571 two-component
RT regulatory system, NtrY/NtrX, involved in nitrogen fixation and
RT metabolism.";
RL Mol. Gen. Genet. 231:124-138(1991).
CC -!- FUNCTION: Part of a potassium transport system. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC -!- DOMAIN: The RCK N-terminal domain binds NAD and possibly other
CC effectors. This is expected to cause a conformation change that
CC regulates potassium transport (By similarity). {ECO:0000250}.
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DR EMBL; AP009384; BAF89080.1; -; Genomic_DNA.
DR EMBL; X63841; CAA45332.1; -; Genomic_DNA.
DR PIR; S18626; S18626.
DR RefSeq; WP_012171606.1; NC_009937.1.
DR AlphaFoldDB; Q04856; -.
DR SMR; Q04856; -.
DR STRING; 438753.AZC_3082; -.
DR EnsemblBacteria; BAF89080; BAF89080; AZC_3082.
DR KEGG; azc:AZC_3082; -.
DR eggNOG; COG0569; Bacteria.
DR HOGENOM; CLU_046525_0_2_5; -.
DR OMA; IACQVAY; -.
DR OrthoDB; 1768241at2; -.
DR Proteomes; UP000000270; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015079; F:potassium ion transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 3.30.70.1450; -; 2.
DR InterPro; IPR006036; K_uptake_TrkA.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR006037; RCK_C.
DR InterPro; IPR036721; RCK_C_sf.
DR InterPro; IPR003148; RCK_N.
DR Pfam; PF02080; TrkA_C; 1.
DR Pfam; PF02254; TrkA_N; 2.
DR PRINTS; PR00335; KUPTAKETRKA.
DR SUPFAM; SSF116726; SSF116726; 2.
DR SUPFAM; SSF51735; SSF51735; 2.
DR PROSITE; PS51202; RCK_C; 2.
DR PROSITE; PS51201; RCK_N; 2.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Ion transport; Membrane; NAD;
KW Potassium; Potassium transport; Reference proteome; Repeat; Transport.
FT CHAIN 1..458
FT /note="Trk system potassium uptake protein TrkA"
FT /id="PRO_0000148711"
FT DOMAIN 2..132
FT /note="RCK N-terminal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00543"
FT DOMAIN 144..228
FT /note="RCK C-terminal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT DOMAIN 235..360
FT /note="RCK N-terminal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00543"
FT DOMAIN 372..453
FT /note="RCK C-terminal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT BINDING 7..11
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /ligand_label="1"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000250"
FT BINDING 30
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /ligand_label="1"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000250"
FT BINDING 73..74
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /ligand_label="1"
FT /ligand_note="ligand shared between dimeric partners"
FT /note="in other chain"
FT /evidence="ECO:0000250"
FT BINDING 98
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /ligand_label="1"
FT /ligand_note="ligand shared between dimeric partners"
FT /evidence="ECO:0000250"
FT BINDING 235..266
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT CONFLICT 95..111
FT /note="KVARIRAQTYLQPEWRS -> SPASAPRPICSPNGA (in Ref. 2;
FT CAA45332)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 458 AA; 49334 MW; C8F21DF257FF28B4 CRC64;
MKVVICGAGQ VGFGIAERLA SEQNDVSIVD ASPRRIQIAT DQLDVRGVVG HGSHPDVLAR
AGIEQADMLI AVTLHDEVNM VACQVGHSLF NVPTKVARIR AQTYLQPEWR SMLSRDHMPI
DVVISPEIEV GEMVLRRLSL PGAVDTVSFG DGSVVIAGVL CGEACPVVDT PLTQLTQLFP
DLPATIVAIN RNGKVMATRS SDQIQTGDIA YFVAPADQVG RTLSIFGHDE IPAKRIIIGG
GGNIGLYVAQ ELEKRNAAAR IKVVESNRER AQAIAEGLSR SVVLHGDSLA REILDEAGVA
DSDTMIALTN DDRVNILSCL LAKQLGAQRI LALVNEQGYA GFAHGLGIDA YVNPRQITVS
KVLQHVRRGR IRGVHSLLDG AGEMIEAEAL ETSPLVGRPL RDIDLLKGMR IGAVVRNGVV
MRPSGDTVVR AQDRVVLFAL ADDIKRVEQL FRVSLEFF
