ID   TRKH_HALED              Reviewed;         482 AA.
AC   E1V6C5; Q6T3V7;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Trk system potassium uptake protein TrkH;
GN   Name=trkH; OrderedLocusNames=HELO_1371;
OS   Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198
OS   / 1H9).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC   Halomonadaceae; Halomonas.
OX   NCBI_TaxID=768066;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   AND GENE NAME.
RC   STRAIN=ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9;
RX   PubMed=15659681; DOI=10.1128/jb.187.3.1036-1043.2005;
RA   Kraegeloh A., Amendt B., Kunte H.J.;
RT   "Potassium transport in a halophilic member of the bacteria domain:
RT   identification and characterization of the K+ uptake systems TrkH and TrkI
RT   from Halomonas elongata DSM 2581T.";
RL   J. Bacteriol. 187:1036-1043(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9;
RX   PubMed=20849449; DOI=10.1111/j.1462-2920.2010.02336.x;
RA   Schwibbert K., Marin-Sanguino A., Bagyan I., Heidrich G., Lentzen G.,
RA   Seitz H., Rampp M., Schuster S.C., Klenk H.P., Pfeiffer F., Oesterhelt D.,
RA   Kunte H.J.;
RT   "A blueprint of ectoine metabolism from the genome of the industrial
RT   producer Halomonas elongata DSM 2581(T).";
RL   Environ. Microbiol. 13:1973-1994(2011).
CC   -!- FUNCTION: Low-affinity potassium transport system. Probably interacts
CC       with Trk system potassium uptake protein TrkA.
CC       {ECO:0000269|PubMed:15659681}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         Vmax=137 nmol/min/mg enzyme {ECO:0000269|PubMed:15659681};
CC         Note=The K(+) uptake does not follow the Michaelis-Menten kinetics.;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TrkH potassium transport family.
CC       {ECO:0000305}.
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DR   EMBL; AY437838; AAR91792.1; -; Genomic_DNA.
DR   EMBL; FN869568; CBV41254.1; -; Genomic_DNA.
DR   AlphaFoldDB; E1V6C5; -.
DR   SMR; E1V6C5; -.
DR   STRING; 768066.HELO_1371; -.
DR   TCDB; 2.A.38.1.3; the k(+) transporter (trk) family.
DR   EnsemblBacteria; CBV41254; CBV41254; HELO_1371.
DR   KEGG; hel:HELO_1371; -.
DR   eggNOG; COG0168; Bacteria.
DR   HOGENOM; CLU_030708_0_2_6; -.
DR   OMA; LQWMGGM; -.
DR   Proteomes; UP000008707; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015379; F:potassium:chloride symporter activity; IEA:InterPro.
DR   InterPro; IPR003445; Cat_transpt.
DR   InterPro; IPR004772; TrkH.
DR   Pfam; PF02386; TrkH; 1.
DR   PIRSF; PIRSF006247; TrkH; 1.
DR   TIGRFAMs; TIGR00933; 2a38; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Ion transport; Membrane; Potassium;
KW   Potassium transport; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..482
FT                   /note="Trk system potassium uptake protein TrkH"
FT                   /id="PRO_0000430038"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        276..296
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        325..345
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        395..415
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        455..475
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   482 AA;  52082 MW;  EA4AB31C1CE5A41C CRC64;
     MSLRMILRIL GLLLMMFSLT MVPPILISLL FADGMWQAFV VALGITVGTG ALMYLPNRHA
     RKELRTRDGF LIAALFWSVL GLFGSLPLML TGAAALSPTD AVFESFSGLT TTGATVITGI
     DLLPEAILYY RQQLQWLGGM GIVVLAVAIL PTLGVGGMAL YRTEIPGPLK DSKLTPRITE
     TAKALWYIYA TLTVTCALAY MAAGMNWFDA LGHSFSTVAI GGFSTHDASI GYFDSAAIEL
     ICSAFLLISA FSFSLHFVAW RERRLTHYFQ DPEARFLMLF LAGLIIITSV SLWLTSDYET
     LQGLRHAVFE VVSIATTAGF SVADFSTWPG ALPFLLFVAA FVGGCSGSTG GGMKVIRIIL
     ILKQGMREVM RLIHPSAVIA VKIGKVSVPD GIAQAVWGFF SAYVLLFFLM LVGVMATGVD
     QVTAWSTVGA TLNNLGPALG EASAHYGDLP SLAKWILVVA MLLGRLEIFT VVVLFTPAFW
     RK