ID   TRKI_HALED              Reviewed;         492 AA.
AC   E1V6K4; Q6T3V6;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Trk system potassium uptake protein TrkI;
GN   Name=trkI; OrderedLocusNames=HELO_1450;
OS   Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198
OS   / 1H9).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC   Halomonadaceae; Halomonas.
OX   NCBI_TaxID=768066;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   AND GENE NAME.
RC   STRAIN=ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9;
RX   PubMed=15659681; DOI=10.1128/jb.187.3.1036-1043.2005;
RA   Kraegeloh A., Amendt B., Kunte H.J.;
RT   "Potassium transport in a halophilic member of the bacteria domain:
RT   identification and characterization of the K+ uptake systems TrkH and TrkI
RT   from Halomonas elongata DSM 2581T.";
RL   J. Bacteriol. 187:1036-1043(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9;
RX   PubMed=20849449; DOI=10.1111/j.1462-2920.2010.02336.x;
RA   Schwibbert K., Marin-Sanguino A., Bagyan I., Heidrich G., Lentzen G.,
RA   Seitz H., Rampp M., Schuster S.C., Klenk H.P., Pfeiffer F., Oesterhelt D.,
RA   Kunte H.J.;
RT   "A blueprint of ectoine metabolism from the genome of the industrial
RT   producer Halomonas elongata DSM 2581(T).";
RL   Environ. Microbiol. 13:1973-1994(2011).
CC   -!- FUNCTION: Medium-affinity potassium transport system. Probably
CC       interacts with Trk system potassium uptake protein TrkA. Main K(+)
CC       transporter in osmotically adapted cells.
CC       {ECO:0000269|PubMed:15659681}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=1.12 mM for K(+) {ECO:0000269|PubMed:15659681};
CC         Vmax=176 nmol/min/mg enzyme {ECO:0000269|PubMed:15659681};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TrkH potassium transport family.
CC       {ECO:0000305}.
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DR   EMBL; AY437839; AAR91793.1; -; Genomic_DNA.
DR   EMBL; FN869568; CBV41333.1; -; Genomic_DNA.
DR   AlphaFoldDB; E1V6K4; -.
DR   SMR; E1V6K4; -.
DR   STRING; 768066.HELO_1450; -.
DR   TCDB; 2.A.38.1.2; the k(+) transporter (trk) family.
DR   EnsemblBacteria; CBV41333; CBV41333; HELO_1450.
DR   KEGG; hel:HELO_1450; -.
DR   eggNOG; COG0168; Bacteria.
DR   HOGENOM; CLU_030708_0_1_6; -.
DR   OMA; GSMNHFS; -.
DR   Proteomes; UP000008707; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015379; F:potassium:chloride symporter activity; IEA:InterPro.
DR   InterPro; IPR003445; Cat_transpt.
DR   InterPro; IPR004772; TrkH.
DR   Pfam; PF02386; TrkH; 1.
DR   PIRSF; PIRSF006247; TrkH; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Ion transport; Membrane; Potassium;
KW   Potassium transport; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..492
FT                   /note="Trk system potassium uptake protein TrkI"
FT                   /id="PRO_0000430039"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        403..423
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        465..485
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   492 AA;  53072 MW;  9D8304BE2C7643EF CRC64;
     MADTRRVTDT LRRWAPILKV LAVLWLVLAI FMAIPLLVLI VESEPDALAF GLSIAIVLAA
     ATLSWIVTWR IPVSLKPWQM FVLTTLSWVT ISSFASLPLV LGAPQLSLTN AVFESVSAIT
     TTGSTILVHI EDLSDGLKLW RGIMQWLGGI GIIVMGIAIL PFLKVGGMRL FHTESSDWSD
     KVMPRTGGIA KATLSIYCGF TLLAAMAYYL GGMSPLDAVV HAMTSLATGG FANSDASFGA
     YAEQPQLLWM GSLFMLCGAL PFVLYIRFLR GSRMALLRDQ QVQGLLLLLL LVILALTIWR
     VSQGTPAFTS LTQVTFNVVS VVTTTGYASD DYSAWGATAY VAFFYLTFVG GCSGSTSGGM
     KIFRFQVAML LLRDQLRYLI HASGVFVSRY NNQPLTDDIT RGVVAFSFFF FLTVAGLALG
     LSLLGLDFTT ALSGAATAVA NVGPGLGETI GPAGNFAPLP DAAKWLLCVG MLMGRLEILT
     VLVLLTPMFW RQ