TRL32_ARATH
ID TRL32_ARATH Reviewed; 192 AA.
AC Q8VZT6; Q9FYD5;
DT 13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Thioredoxin-like 3-2, chloroplastic;
DE AltName: Full=Thioredoxin WCRKC-2;
DE Flags: Precursor;
GN Name=WCRKC2; OrderedLocusNames=At5g04260; ORFNames=F21E1_180;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19825616; DOI=10.1093/mp/ssn076;
RA Chibani K., Wingsle G., Jacquot J.P., Gelhaye E., Rouhier N.;
RT "Comparative genomic study of the thioredoxin family in photosynthetic
RT organisms with emphasis on Populus trichocarpa.";
RL Mol. Plant 2:308-322(2009).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=19259774; DOI=10.1007/s11103-009-9471-4;
RA Cain P., Hall M., Schroder W.P., Kieselbach T., Robinson C.;
RT "A novel extended family of stromal thioredoxins.";
RL Plant Mol. Biol. 70:273-281(2009).
CC -!- FUNCTION: Probable thiol-disulfide oxidoreductase that may participate
CC in various redox reactions.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000269|PubMed:19259774}.
CC -!- SIMILARITY: Belongs to the thioredoxin family. {ECO:0000305}.
CC -!- CAUTION: The active site contains a CRKC motif wich differs from the
CC conserved CGPC motif. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC05508.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL391716; CAC05508.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90720.1; -; Genomic_DNA.
DR EMBL; AY063850; AAL36206.1; -; mRNA.
DR EMBL; AY117290; AAM51365.1; -; mRNA.
DR RefSeq; NP_196046.2; NM_120508.4.
DR AlphaFoldDB; Q8VZT6; -.
DR SMR; Q8VZT6; -.
DR STRING; 3702.AT5G04260.1; -.
DR PaxDb; Q8VZT6; -.
DR PRIDE; Q8VZT6; -.
DR ProteomicsDB; 245237; -.
DR EnsemblPlants; AT5G04260.1; AT5G04260.1; AT5G04260.
DR GeneID; 830305; -.
DR Gramene; AT5G04260.1; AT5G04260.1; AT5G04260.
DR KEGG; ath:AT5G04260; -.
DR Araport; AT5G04260; -.
DR TAIR; locus:2146693; AT5G04260.
DR eggNOG; KOG0907; Eukaryota.
DR HOGENOM; CLU_120169_0_0_1; -.
DR InParanoid; Q8VZT6; -.
DR OMA; GPICGES; -.
DR OrthoDB; 1218391at2759; -.
DR PhylomeDB; Q8VZT6; -.
DR PRO; PR:Q8VZT6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VZT6; baseline and differential.
DR Genevisible; Q8VZT6; AT.
DR GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR GO; GO:0045454; P:cell redox homeostasis; IDA:TAIR.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR InterPro; IPR013766; Thioredoxin_domain.
DR InterPro; IPR044253; WCRKC1/2.
DR PANTHER; PTHR47192; PTHR47192; 1.
DR Pfam; PF00085; Thioredoxin; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS51352; THIOREDOXIN_2; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Disulfide bond; Electron transport; Plastid;
KW Redox-active center; Reference proteome; Transit peptide; Transport.
FT TRANSIT 1..55
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 56..192
FT /note="Thioredoxin-like 3-2, chloroplastic"
FT /id="PRO_0000034172"
FT DOMAIN 66..191
FT /note="Thioredoxin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT ACT_SITE 110
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT ACT_SITE 113
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT DISULFID 110..113
FT /note="Redox-active"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
SQ SEQUENCE 192 AA; 21836 MW; 3F9459B9AA060070 CRC64;
MSEIVNLSSS LRSLNPKISP LVPPYRQTSS SFSRPRNFKY HSFTDKICLA AERIRAVDIQ
KQDGGLQELD DSPVSVELGP ICGESHFDQV MEDAQKLGES VVIVWMAAWC RKCIYLKPKL
EKLAAEFYPR LRFYHVDVNA VPYRLVSRAG VTKMPTIQLW RDGQKQAEVI GGHKAHFVVN
EVREMIENDS IT
