TRL4_ARATH
ID TRL4_ARATH Reviewed; 204 AA.
AC Q9C5C5; Q3EDG8; Q3EDG9; Q9LQP5;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Thioredoxin-like 4, chloroplastic;
DE AltName: Full=Lilium-type thioredoxin 3;
DE Flags: Precursor;
GN OrderedLocusNames=At1g07700; ORFNames=F24B9.21;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19825616; DOI=10.1093/mp/ssn076;
RA Chibani K., Wingsle G., Jacquot J.P., Gelhaye E., Rouhier N.;
RT "Comparative genomic study of the thioredoxin family in photosynthetic
RT organisms with emphasis on Populus trichocarpa.";
RL Mol. Plant 2:308-322(2009).
CC -!- FUNCTION: Probable thiol-disulfide oxidoreductase that may participate
CC in various redox reactions.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9C5C5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9C5C5-2; Sequence=VSP_039284;
CC Name=3;
CC IsoId=Q9C5C5-3; Sequence=VSP_039285, VSP_039286;
CC -!- SIMILARITY: Belongs to the thioredoxin family. {ECO:0000305}.
CC -!- CAUTION: The active site contains a CGSC motif wich differs from the
CC conserved CGPC motif. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF75085.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BX815031; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=BX816726; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC007583; AAF75085.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28163.2; -; Genomic_DNA.
DR EMBL; CP002684; AEE28164.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28165.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59501.1; -; Genomic_DNA.
DR EMBL; AF360345; AAK28642.1; -; mRNA.
DR EMBL; AY051082; AAK93759.1; -; mRNA.
DR EMBL; BX815031; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BX816726; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK229680; BAF01521.1; -; mRNA.
DR PIR; A86212; A86212.
DR RefSeq; NP_001318945.1; NM_001331721.1. [Q9C5C5-1]
DR RefSeq; NP_001321855.1; NM_001331722.1. [Q9C5C5-1]
DR RefSeq; NP_563794.1; NM_100643.4. [Q9C5C5-1]
DR RefSeq; NP_973781.1; NM_202052.2. [Q9C5C5-2]
DR AlphaFoldDB; Q9C5C5; -.
DR SMR; Q9C5C5; -.
DR STRING; 3702.AT1G07700.3; -.
DR PaxDb; Q9C5C5; -.
DR PRIDE; Q9C5C5; -.
DR ProteomicsDB; 245238; -. [Q9C5C5-1]
DR EnsemblPlants; AT1G07700.1; AT1G07700.1; AT1G07700. [Q9C5C5-1]
DR EnsemblPlants; AT1G07700.2; AT1G07700.2; AT1G07700. [Q9C5C5-1]
DR EnsemblPlants; AT1G07700.3; AT1G07700.3; AT1G07700. [Q9C5C5-2]
DR EnsemblPlants; AT1G07700.4; AT1G07700.4; AT1G07700. [Q9C5C5-1]
DR GeneID; 837283; -.
DR Gramene; AT1G07700.1; AT1G07700.1; AT1G07700. [Q9C5C5-1]
DR Gramene; AT1G07700.2; AT1G07700.2; AT1G07700. [Q9C5C5-1]
DR Gramene; AT1G07700.3; AT1G07700.3; AT1G07700. [Q9C5C5-2]
DR Gramene; AT1G07700.4; AT1G07700.4; AT1G07700. [Q9C5C5-1]
DR KEGG; ath:AT1G07700; -.
DR Araport; AT1G07700; -.
DR TAIR; locus:2026504; AT1G07700.
DR eggNOG; KOG2151; Eukaryota.
DR HOGENOM; CLU_097713_1_0_1; -.
DR InParanoid; Q9C5C5; -.
DR OMA; FISNVEW; -.
DR OrthoDB; 1401899at2759; -.
DR PhylomeDB; Q9C5C5; -.
DR PRO; PR:Q9C5C5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C5C5; baseline and differential.
DR Genevisible; Q9C5C5; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR InterPro; IPR013766; Thioredoxin_domain.
DR InterPro; IPR044176; TRL4_chloroplastic.
DR PANTHER; PTHR47912; PTHR47912; 1.
DR Pfam; PF00085; Thioredoxin; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS51352; THIOREDOXIN_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Disulfide bond; Electron transport;
KW Plastid; Redox-active center; Reference proteome; Transit peptide;
KW Transport.
FT TRANSIT 1..27
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 28..204
FT /note="Thioredoxin-like 4, chloroplastic"
FT /id="PRO_0000394542"
FT DOMAIN 63..201
FT /note="Thioredoxin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT ACT_SITE 119
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT ACT_SITE 122
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT DISULFID 119..122
FT /note="Redox-active"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT VAR_SEQ 1..6
FT /note="MSSLLN -> MTPLLWFCFKILIFDSNLQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_039284"
FT VAR_SEQ 168..171
FT /note="AVPL -> VIPL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_039285"
FT VAR_SEQ 172..204
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_039286"
SQ SEQUENCE 204 AA; 23011 MW; 169D801F486493FC CRC64;
MSSLLNISHC SYHGYSGLTS RGGINTVENH RWVWHNNGVR LSFPRAESSI NITMGCTLQR
GIAKSLSQEN LVELSDENDD LCPVECVTEF KTDDELLSVL EKSKETNSLV VVDFYRTACG
SCKYIEQGFS KLCKQSGDQE APVIFLKHNV VDEYDEQSEV AERLRIKAVP LFHFYKNGVL
LESFATRDKE RIDAAILKYT SSES
