TRM1_PYRHO
ID TRM1_PYRHO Reviewed; 381 AA.
AC O59493;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=tRNA (guanine(26)-N(2))-dimethyltransferase {ECO:0000255|HAMAP-Rule:MF_00290};
DE EC=2.1.1.216 {ECO:0000255|HAMAP-Rule:MF_00290};
DE AltName: Full=tRNA 2,2-dimethylguanosine-26 methyltransferase {ECO:0000255|HAMAP-Rule:MF_00290};
DE AltName: Full=tRNA(guanine-26,N(2)-N(2)) methyltransferase {ECO:0000255|HAMAP-Rule:MF_00290};
DE AltName: Full=tRNA(m(2,2)G26)dimethyltransferase {ECO:0000255|HAMAP-Rule:MF_00290};
GN Name=trm1 {ECO:0000255|HAMAP-Rule:MF_00290}; OrderedLocusNames=PH1829;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- FUNCTION: Dimethylates a single guanine residue at position 26 of a
CC number of tRNAs using S-adenosyl-L-methionine as donor of the methyl
CC groups. {ECO:0000255|HAMAP-Rule:MF_00290}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) +
CC N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43140, Rhea:RHEA-COMP:10359, Rhea:RHEA-COMP:10360,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74513; EC=2.1.1.216;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00290};
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Trm1 family. {ECO:0000255|HAMAP-Rule:MF_00290}.
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DR EMBL; BA000001; BAA30948.1; -; Genomic_DNA.
DR PIR; E71194; E71194.
DR PDB; 2DUL; X-ray; 1.90 A; A=4-381.
DR PDB; 2EJT; X-ray; 2.20 A; A=4-381.
DR PDB; 2EJU; X-ray; 1.95 A; A=4-381.
DR PDB; 2YTZ; X-ray; 2.65 A; A/B=4-381.
DR PDBsum; 2DUL; -.
DR PDBsum; 2EJT; -.
DR PDBsum; 2EJU; -.
DR PDBsum; 2YTZ; -.
DR AlphaFoldDB; O59493; -.
DR SMR; O59493; -.
DR STRING; 70601.3258265; -.
DR PRIDE; O59493; -.
DR EnsemblBacteria; BAA30948; BAA30948; BAA30948.
DR KEGG; pho:PH1829; -.
DR eggNOG; arCOG01219; Archaea.
DR OMA; VFYNPVM; -.
DR BRENDA; 2.1.1.216; 5244.
DR EvolutionaryTrace; O59493; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR Gene3D; 3.30.56.70; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_00290; tRNA_dimethyltr_TRM1; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002905; Trm1.
DR InterPro; IPR022923; TRM1_arc_bac.
DR InterPro; IPR042296; tRNA_met_Trm1_C.
DR PANTHER; PTHR10631; PTHR10631; 2.
DR Pfam; PF02005; TRM; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR00308; TRM1; 1.
DR PROSITE; PS51626; SAM_MT_TRM1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Methyltransferase; RNA-binding; S-adenosyl-L-methionine;
KW Transferase; tRNA processing; tRNA-binding.
FT CHAIN 1..381
FT /note="tRNA (guanine(26)-N(2))-dimethyltransferase"
FT /id="PRO_0000147690"
FT DOMAIN 7..378
FT /note="Trm1 methyltransferase"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00290"
FT STRAND 7..11
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 14..18
FT /evidence="ECO:0007829|PDB:2DUL"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:2YTZ"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:2YTZ"
FT HELIX 33..35
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 36..49
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 52..58
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 63..71
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 75..82
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 84..97
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 103..105
FT /evidence="ECO:0007829|PDB:2YTZ"
FT STRAND 106..122
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 124..130
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 135..140
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 147..156
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 157..167
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 170..173
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 178..185
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 186..188
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 195..212
FT /evidence="ECO:0007829|PDB:2DUL"
FT TURN 213..215
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 216..226
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 229..239
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 240..247
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 250..255
FT /evidence="ECO:0007829|PDB:2DUL"
FT TURN 257..259
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 262..269
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 272..274
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 276..280
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 287..298
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 305..317
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 327..334
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 341..350
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 355..358
FT /evidence="ECO:0007829|PDB:2DUL"
FT STRAND 361..369
FT /evidence="ECO:0007829|PDB:2DUL"
FT HELIX 371..378
FT /evidence="ECO:0007829|PDB:2DUL"
SQ SEQUENCE 381 AA; 43271 MW; 7D28AB17F805450E CRC64;
MVNLELIEVQ EGKAKILIPK AESIYDSPVF YNPRMALNRD IVVVLLNILN PKIVLDALSA
TGIRGIRFAL ETPAEEVWLN DISEDAYELM KRNVMLNFDG ELRESKGRAI LKGEKTIVIN
HDDANRLMAE RHRYFHFIDL DPFGSPMEFL DTALRSAKRR GILGVTATDG APLCGAHPRA
CLRKYLAVPL RGELCHEVGT RILVGVIARY AAKYDLGIDV ILAYYKDHYF RAFVKLKDGA
RKGDETLEKL GYIYFDDKTG KFELEQGFLP TRPNAYGPVW LGPLKDEKIV SKMVKEAESL
SLARKKQALK LLKMIDQELD IPLFYDTHAI GRRLKIETKK VEEIISALRE QGYEATRTHF
SPTGIKTSAP YEVFIETIKR I