ID   TRM3_ALHV1              Reviewed;         686 AA.
AC   O36378;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Tripartite terminase subunit 3 {ECO:0000255|HAMAP-Rule:MF_04013};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_04013};
DE   AltName: Full=Terminase large subunit {ECO:0000255|HAMAP-Rule:MF_04013};
GN   Name=TRM3 {ECO:0000255|HAMAP-Rule:MF_04013}; OrderedLocusNames=29;
OS   Alcelaphine herpesvirus 1 (strain C500) (AlHV-1) (Malignant catarrhal fever
OS   virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Macavirus.
OX   NCBI_TaxID=654901;
OH   NCBI_TaxID=9927; Connochaetes taurinus (Blue wildebeest).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9261371; DOI=10.1128/jvi.71.9.6517-6525.1997;
RA   Ensser A., Pflanz R., Fleckenstein B.;
RT   "Primary structure of the alcelaphine herpesvirus 1 genome.";
RL   J. Virol. 71:6517-6525(1997).
CC   -!- FUNCTION: Component of the molecular motor that translocates viral
CC       genomic DNA in empty capsid during DNA packaging. Forms a tripartite
CC       terminase complex together with TRM1 and TRM2 in the host cytoplasm.
CC       Once the complex reaches the host nucleus, it interacts with the capsid
CC       portal vertex. This portal forms a ring in which genomic DNA is
CC       translocated into the capsid. TRM3 carries an RNase H-like nuclease
CC       activity that plays an important role for the cleavage of concatemeric
CC       viral DNA into unit length genomes. {ECO:0000255|HAMAP-Rule:MF_04013}.
CC   -!- SUBUNIT: Interacts with the terminase subunits TRM1 and TRM2. Interacts
CC       with portal protein. {ECO:0000255|HAMAP-Rule:MF_04013}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04013}.
CC       Note=Responsible for the nuclear localization of the two others
CC       subunits TRM1 and TRM2. {ECO:0000255|HAMAP-Rule:MF_04013}.
CC   -!- SIMILARITY: Belongs to the herpesviridae TRM3 protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04013}.
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DR   EMBL; AF005370; AAC58075.1; -; Genomic_DNA.
DR   PIR; T03123; T03123.
DR   RefSeq; NP_065527.1; NC_002531.1.
DR   SMR; O36378; -.
DR   GeneID; 911744; -.
DR   KEGG; vg:911744; -.
DR   Proteomes; UP000000941; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0051276; P:chromosome organization; IEA:InterPro.
DR   GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.420.320; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_04013; HSV_TRM3; 1.
DR   InterPro; IPR003498; DNA_pack_C.
DR   InterPro; IPR038435; DNA_pack_C_sf.
DR   InterPro; IPR003499; DNA_pack_N.
DR   InterPro; IPR033663; HSV_TRM3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF02499; DNA_pack_C; 1.
DR   Pfam; PF02500; DNA_pack_N; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Host nucleus; Hydrolase; Reference proteome;
KW   Viral genome packaging; Viral release from host cell.
FT   CHAIN           1..686
FT                   /note="Tripartite terminase subunit 3"
FT                   /id="PRO_0000405716"
FT   MOTIF           220..227
FT                   /note="Walker A motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   MOTIF           315..320
FT                   /note="Walker B motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   ACT_SITE        320
FT                   /note="For ATPase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   ACT_SITE        474
FT                   /note="For nuclease activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   ACT_SITE        546
FT                   /note="For nuclease activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   ACT_SITE        658
FT                   /note="For nuclease activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
SQ   SEQUENCE   686 AA;  76860 MW;  BA86EEBEFC202460 CRC64;
     MFYVKVMPAL QKACEELQNQ WSAKSGKWPV PETPLVAVET RRSERWPHPY LGLLPGVAAY
     SSTLEDYCHL YNPYIDALTR CDLGQTHRRV ATQPVLSDQL CQQLKKLFSC PRNTSVKAKL
     EFEAAVRTHQ ALDNSQVFLE LKTFVLNLSA FLNKRYSDRS SHIELFQKQL IMHTFFFLVS
     IKAPELCEKF CNIFKLYFNI DTMDQATLDI FKQKASVFLI PRRHGKTWIV VAIISILLAS
     VQDLRIGYVA HQKHVANAVF TEVINTLHTF FPGKYMDVKK ENGTIIFGLP NKKPSTLLCA
     TCFNKNSIRG QTFQLLFVDE ANFIKKDALP TILGFMLQKD AKIIFISSSN SSDQSTSFLY
     NLKGASERML NVVSYVCSNH KEDFSMQDGL ISCPCYSLHV PSYISIDEQI KTTTNLFLDG
     VFDTELMGDS SCGTLSTFQI ISESALSQFE LCRIDTASPQ VQAHLNSTVH MYIDPAFTNN
     LDASGTGISV IGRLGAKTKV ILGCEHFFLQ KLTGTAALQI ASCATSLLRS VVIIHPMIKC
     AQITIEGNSS QDSAVAIANF IDECAPIPVT FYHQSDKTKG VLCPLYLLGQ EKAVAFESFI
     YAMNLGLCKA SQLIVSHTIK LSFDPVTYLL EQVRAIKCQS LRDGSHTYHA KQKNLSDDLL
     VSVVMSLYLS SANTLPFKPL HIERFF