ID   TRM3_VZVD               Reviewed;         747 AA.
AC   P09294;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Tripartite terminase subunit 3 {ECO:0000255|HAMAP-Rule:MF_04013};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_04013};
DE   AltName: Full=Terminase large subunit {ECO:0000255|HAMAP-Rule:MF_04013};
DE   Contains:
DE     RecName: Full=Gene 42 protein;
GN   Name=TRM3 {ECO:0000255|HAMAP-Rule:MF_04013}; OrderedLocusNames=45/42;
OS   Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=10338;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=3018124; DOI=10.1099/0022-1317-67-9-1759;
RA   Davison A.J., Scott J.E.;
RT   "The complete DNA sequence of varicella-zoster virus.";
RL   J. Gen. Virol. 67:1759-1816(1986).
CC   -!- FUNCTION: Component of the molecular motor that translocates viral
CC       genomic DNA in empty capsid during DNA packaging. Forms a tripartite
CC       terminase complex together with TRM1 and TRM2 in the host cytoplasm.
CC       Once the complex reaches the host nucleus, it interacts with the capsid
CC       portal vertex. This portal forms a ring in which genomic DNA is
CC       translocated into the capsid. TRM3 carries an RNase H-like nuclease
CC       activity that plays an important role for the cleavage of concatemeric
CC       viral DNA into unit length genomes. {ECO:0000255|HAMAP-Rule:MF_04013}.
CC   -!- SUBUNIT: Interacts with the terminase subunits TRM1 and TRM2. Interacts
CC       with portal protein. {ECO:0000255|HAMAP-Rule:MF_04013}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04013}.
CC       Note=Responsible for the nuclear localization of the two others
CC       subunits TRM1 and TRM2. {ECO:0000255|HAMAP-Rule:MF_04013}.
CC   -!- SIMILARITY: Belongs to the herpesviridae TRM3 protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04013}.
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DR   EMBL; X04370; CAB55553.1; -; Genomic_DNA.
DR   PIR; A27344; WZBE45.
DR   PIR; G27341; G27341.
DR   SMR; P09294; -.
DR   PRIDE; P09294; -.
DR   Proteomes; UP000002602; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0051276; P:chromosome organization; IEA:InterPro.
DR   GO; GO:0019076; P:viral release from host cell; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.420.320; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_04013; HSV_TRM3; 1.
DR   InterPro; IPR003498; DNA_pack_C.
DR   InterPro; IPR038435; DNA_pack_C_sf.
DR   InterPro; IPR003499; DNA_pack_N.
DR   InterPro; IPR033663; HSV_TRM3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF02499; DNA_pack_C; 1.
DR   Pfam; PF02500; DNA_pack_N; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Host nucleus; Hydrolase; Reference proteome;
KW   Viral genome packaging; Viral release from host cell.
FT   CHAIN           1..747
FT                   /note="Tripartite terminase subunit 3"
FT                   /id="PRO_0000038308"
FT   CHAIN           353..747
FT                   /note="Gene 42 protein"
FT                   /id="PRO_0000038309"
FT   MOTIF           194..198
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   MOTIF           267..274
FT                   /note="Walker A motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   MOTIF           361..366
FT                   /note="Walker B motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   ACT_SITE        366
FT                   /note="For ATPase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   ACT_SITE        521
FT                   /note="For nuclease activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   ACT_SITE        593
FT                   /note="For nuclease activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
FT   ACT_SITE        722
FT                   /note="For nuclease activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04013"
SQ   SEQUENCE   747 AA;  82757 MW;  1A056546B9BD5194 CRC64;
     MSLIMFGRTL GEESVRYFER LKRRRDERFG TLESPTPCST RQGSLGNATQ IPFLNFAIDV
     TRRHQAVIPG IGTLHNCCEY IPLFSATARR AMFGAFLSST GYNCTPNVVL KPWRYSVNAN
     VSPELKKAVS SVQFYEYSPE EAAPHRNAYS GVMNTFRAFS LSDSFCQLST FTQRFSYLVE
     TSFESIEECG SHGKRAKVDV PIYGRYKGTL ELFQKMILMH TTHFISSVLL GDHADRVDCF
     LRTVFNTPSV SDSVLEHFKQ KSTVFLVPRR HGKTWFLVPL IALVMATFRG IKVGYTAHIR
     KATEPVFEGI KSRLEQWFGA NYVDHVKGES ITFSFTDGSY STAVFASSHN TNGIRGQDFN
     LLFVDEANFI RPDAVQTIVG FLNQTNCKII FVSSTNTGKA STSFLYNLRG SSDQLLNVVT
     YVCDDHMPRV LAHSDVTACS CYVLNKPVFI TMDGAMRRTA DLFMADSFVQ EIVGGRKQNS
     GGVGFDRPLF TKTARERFIL YRPSTVANCA ILSSVLYVYV DPAFTSNTRA SGTGVAIVGR
     YKSDWIIFGL EHFFLRALTG TSSSEIGRCV TQCLGHILAL HPNTFTNVHV SIEGNSSQDS
     AVAISLAIAQ QFAVLEKGNV LSSAPVLLFY HSIPPGCSVA YPFFLLQKQK TPAVDYFVKR
     FNSGNIIASQ ELVSLTVKLG VDPVEYLCKQ LDNLTEVIKG GMGNLDTKTY TGKGTTGTMS
     DDLMVALIMS VYIGSSCIPD SVFMPIK