TRM44_ASPFU
ID TRM44_ASPFU Reviewed; 650 AA.
AC Q4WCV5;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=tRNA (uracil-O(2)-)-methyltransferase;
DE EC=2.1.1.211;
GN Name=trm44; ORFNames=AFUA_6G02570;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
CC -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAHF01000012; EAL85783.1; -; Genomic_DNA.
DR RefSeq; XP_747821.1; XM_742728.1.
DR AlphaFoldDB; Q4WCV5; -.
DR STRING; 746128.CADAFUBP00009305; -.
DR EnsemblFungi; EAL85783; EAL85783; AFUA_6G02570.
DR GeneID; 3505098; -.
DR KEGG; afm:AFUA_6G02570; -.
DR VEuPathDB; FungiDB:Afu6g02570; -.
DR eggNOG; KOG3790; Eukaryota.
DR HOGENOM; CLU_018580_0_0_1; -.
DR InParanoid; Q4WCV5; -.
DR OMA; WIPLLGY; -.
DR OrthoDB; 1146281at2759; -.
DR Proteomes; UP000002530; Chromosome 6.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016300; F:tRNA (uracil) methyltransferase activity; IBA:GO_Central.
DR GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR PANTHER; PTHR21210; PTHR21210; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase; tRNA processing.
FT CHAIN 1..650
FT /note="tRNA (uracil-O(2)-)-methyltransferase"
FT /id="PRO_0000249901"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 91..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 481..513
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..112
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 650 AA; 72946 MW; 5FF26E494EECEC8B CRC64;
MPRNVKKNPR DVTKLSGKPL AETIEPSPVL KTLQEGWVTS PDLIEGDLSF TLEVIQNMTV
FLLANPNLNS SHLFRADILY DSLGVLSAPH AMEQSRDQGS GGRDQDTQKE DYVEPLPARS
VPGFELKRTF VRRLIPRNRN LDRPLEQTCH FYEADELPPT SNSTNIAQSE QCESKAESPR
QRFLVIYLPH VSSKEEMPWY HPLLRSLAFL YDFTPNAFPH SSEENKGTGI MSVHFLPYEE
EPIQNRLERT LLALLSTHIR LARQTRLDEK PEGGNYNPHK DNVIPKHLVQ DTYARLKLKY
AADLCQNWVE DTEPTKHVFE DLSITAFLIE LWRSMYGVVP ADERSPDQKE QGKYDLKFPG
FVDVACGNGV LVYVLLSEGY HGWGFDARRR KTWSIFPSSI QERLKEEIYI PKPFADVCTD
SEMDFGVKTH TGMFPEHTFI ISNHADELTV WTPLMAALSN PASPLPFISI PCCSHSLSGA
RYRYPPPKPN QKGSVDRPED QEVEQNPQPA AGDLKALRAA KQKERTDTGA ANSMYGSLTA
KTVATAEEIG YEVEKTLLRI PSTRNMGVIG GRRRTTQEWT SRVHSAQASQ AVGCSAPEAV
RAAAQVLEWV AAVVQRECAR EGGVEVAAKV WIERAKGLHK GQGKGNQPAH
