ID   TRM44_ASPOR             Reviewed;         635 AA.
AC   Q2U6Q1;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=tRNA (uracil-O(2)-)-methyltransferase;
DE            EC=2.1.1.211;
GN   Name=trm44; ORFNames=AO090120000142;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC       (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC         methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
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DR   EMBL; AP007166; BAE62764.1; -; Genomic_DNA.
DR   RefSeq; XP_001823897.1; XM_001823845.2.
DR   AlphaFoldDB; Q2U6Q1; -.
DR   STRING; 510516.Q2U6Q1; -.
DR   EnsemblFungi; BAE62764; BAE62764; AO090120000142.
DR   GeneID; 5996156; -.
DR   KEGG; aor:AO090120000142; -.
DR   VEuPathDB; FungiDB:AO090120000142; -.
DR   HOGENOM; CLU_018580_0_0_1; -.
DR   OMA; WIPLLGY; -.
DR   Proteomes; UP000006564; Chromosome 5.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR   InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR   PANTHER; PTHR21210; PTHR21210; 1.
DR   Pfam; PF07757; AdoMet_MTase; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..635
FT                   /note="tRNA (uracil-O(2)-)-methyltransferase"
FT                   /id="PRO_0000249902"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          474..509
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        478..509
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   635 AA;  70783 MW;  F69FD99A24CA2211 CRC64;
     MKNHRNKTVR DVTKLSGKPL PETLEPSSIL QGTPTEQWVT SKDLLEDGLP FIPEVMKDLT
     VFLLGNININ STHLFRADIL YDSQGVLSTP QQKELSFAQT GNTSVETTTD AEDRVEPIVA
     AEVAGFNLTR TVVRRLIPRN PKLDRPLEQT CHFYEADIAP GSEGATQESR LRRFLAVYTP
     HVASKEEIPF YHPLLRALAY LYDFTDDAAE ATEAGSGSGA LSLHFLPYPE EALPTRLERT
     LHALLNTQIR LARNTRLSET TEGGNYNPSK DNVIPQHLVQ NTYSRLKFKY GKDLCRDWVE
     DTEPTKHVFE DLAITAFLIE LWRSMYGAVP GEERNEDGPE KYDPNFPGFV DVACGNGVLV
     YVLLMEGYRG WGFDARRRKT WKILPEFVQA RLKEEIYIPK PFTDAMAERG GVPDLGVETH
     SGLFEKDTFI ISNHADELTV WTPLMATLAC PESPLPFIAI PCCSHALSGA KYRYPPPKAS
     KPDSDKESSQ HEDVDSEQPA TGDLKALRKA KQEAQTDVGF YKSMYGSLTM KAISIAEEIG
     YDVEKTLLRI PSTRNMGVIG GRKRVTKEWR ARNQQQPASD CNGDAVTESA LDKAMAAVQR
     ECSRDGGVEG AAKIWVERAK GIHKGQGPGN QRGHC