ID   TRM44_CAEBR             Reviewed;         562 AA.
AC   Q60K16; A8WKC3;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Probable tRNA (uracil-O(2)-)-methyltransferase;
DE            EC=2.1.1.211;
GN   ORFNames=CBG24265;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC       (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC         methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
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DR   EMBL; HE601461; CAP20917.1; -; Genomic_DNA.
DR   RefSeq; XP_002648179.1; XM_002648133.1.
DR   AlphaFoldDB; Q60K16; -.
DR   STRING; 6238.CBG24265; -.
DR   PRIDE; Q60K16; -.
DR   EnsemblMetazoa; CBG24265.1; CBG24265.1; WBGene00042420.
DR   GeneID; 8590182; -.
DR   KEGG; cbr:CBG_24265; -.
DR   CTD; 8590182; -.
DR   WormBase; CBG24265; CBP12807; WBGene00042420; -.
DR   eggNOG; KOG3790; Eukaryota.
DR   HOGENOM; CLU_021025_0_0_1; -.
DR   InParanoid; Q60K16; -.
DR   OMA; ECGGIKT; -.
DR   OrthoDB; 1146281at2759; -.
DR   Proteomes; UP000008549; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016300; F:tRNA (uracil) methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR   InterPro; IPR000571; Znf_CCCH.
DR   PANTHER; PTHR21210; PTHR21210; 1.
DR   Pfam; PF07757; AdoMet_MTase; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS50103; ZF_C3H1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Metal-binding; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing; Zinc; Zinc-finger.
FT   CHAIN           1..562
FT                   /note="Probable tRNA (uracil-O(2)-)-methyltransferase"
FT                   /id="PRO_0000249897"
FT   ZN_FING         535..562
FT                   /note="C3H1-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT   REGION          520..546
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        520..541
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   562 AA;  64910 MW;  A5196A7BC8149119 CRC64;
     MSPWRCIAEQ LVEYEEPVDA KAYFDKIICL WKEYPNAISN RINTSVPVEE ESQVWNEVIA
     NFESKYRFDP ANSSELSIFK LIQRDYKRQS FSNNCYEVAF YEEELLVRFY PIVLDGQQHP
     HFESPYSIAC KIPSNSSILL QFFKPVDTPD DHFEFLKTLA FKQLVTWLKS IDLSKTTRKT
     HSLLDKESYI STYRHIREDL GRPLVEGWTE NSKPQKVIFE DCGIASYIQE LITSGILPKP
     RKFVDIGCGN GLLVHLLNKI GIPGYGVDVR SRKVWKTTLS HVDLRESPVD PQIVVENRPH
     FDADVDLLIG NHSDELTPWI PVMAAKLNCN FFLIPCCPYN FFGKYLNNGS HLGPKRMTSQ
     YESFFEWTVS VSERLGFDTK MDRLAIPSTK RLCIIGRVPE KGLCPSLEQT IEHMTQGQKF
     VARPREIRNN NCMHISVTDR ERIAKKLFDF ILNASDDVRD GWRCGGEVPL AQLAGQLTDD
     DKKLMKDQDG GLQTFLRNHH QIFHVFQATA RLRDFRQPVV SRRQQTNPKK QEATNRPKQP
     CWMSLNHPDG CPLGPESCRY LH