TRM44_CAEEL
ID TRM44_CAEEL Reviewed; 563 AA.
AC Q45EK7; Q45EK5; Q45EK6;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Probable tRNA (uracil-O(2)-)-methyltransferase;
DE EC=2.1.1.211;
GN ORFNames=C23G10.7;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=c;
CC IsoId=Q45EK7-1; Sequence=Displayed;
CC Name=a;
CC IsoId=Q45EK7-2; Sequence=VSP_020593, VSP_020594;
CC Name=b;
CC IsoId=Q45EK7-3; Sequence=VSP_020595;
CC -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
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DR EMBL; FO080630; CCD65300.1; -; Genomic_DNA.
DR EMBL; FO080630; CCD65301.1; -; Genomic_DNA.
DR EMBL; FO080630; CCD65302.1; -; Genomic_DNA.
DR RefSeq; NP_001033350.1; NM_001038261.4. [Q45EK7-1]
DR RefSeq; NP_741175.2; NM_171153.4.
DR RefSeq; NP_741176.2; NM_171872.3. [Q45EK7-3]
DR AlphaFoldDB; Q45EK7; -.
DR STRING; 6239.C23G10.7b; -.
DR EPD; Q45EK7; -.
DR PaxDb; Q45EK7; -.
DR PeptideAtlas; Q45EK7; -.
DR PRIDE; Q45EK7; -.
DR EnsemblMetazoa; C23G10.7b.1; C23G10.7b.1; WBGene00016014. [Q45EK7-3]
DR EnsemblMetazoa; C23G10.7c.1; C23G10.7c.1; WBGene00016014. [Q45EK7-1]
DR GeneID; 175876; -.
DR KEGG; cel:CELE_C23G10.7; -.
DR UCSC; C23G10.7c; c. elegans. [Q45EK7-1]
DR CTD; 175876; -.
DR WormBase; C23G10.7a; CE38937; WBGene00016014; -. [Q45EK7-2]
DR WormBase; C23G10.7b; CE38938; WBGene00016014; -. [Q45EK7-3]
DR WormBase; C23G10.7c; CE38939; WBGene00016014; -. [Q45EK7-1]
DR eggNOG; KOG3790; Eukaryota.
DR GeneTree; ENSGT00390000000645; -.
DR HOGENOM; CLU_021025_0_0_1; -.
DR InParanoid; Q45EK7; -.
DR OMA; ECGGIKT; -.
DR OrthoDB; 1146281at2759; -.
DR PhylomeDB; Q45EK7; -.
DR PRO; PR:Q45EK7; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00016014; Expressed in adult organism and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016300; F:tRNA (uracil) methyltransferase activity; IBA:GO_Central.
DR GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR PANTHER; PTHR21210; PTHR21210; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Cytoplasm; Metal-binding; Methyltransferase;
KW Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing;
KW Zinc; Zinc-finger.
FT CHAIN 1..563
FT /note="Probable tRNA (uracil-O(2)-)-methyltransferase"
FT /id="PRO_0000249898"
FT ZN_FING 536..563
FT /note="C3H1-type"
FT VAR_SEQ 395..408
FT /note="LCIIGRVPEGGLCP -> KEDYAQMWTKPSIQ (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_020593"
FT VAR_SEQ 409..563
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_020594"
FT VAR_SEQ 507
FT /note="H -> HVSTTAIDKK (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_020595"
SQ SEQUENCE 563 AA; 64872 MW; 9E937843AE767BAA CRC64;
MFPGSTWTVI VEQIAEYEEV IDPKAFFDKI ISLWKEYPNA ISNRITTSVS VDTDSDLWNE
VLRTFETHYK FCPRDCSEIA ITKIIHKDLA NNKFSDNAFE VSYYEEELLV RFYPITLDGM
QHPHFESPYS IAIKIPTDTS IQLQFLKQAN SSQEHFEFMK KQAFKQLYTW LKGIDLSKSS
RKTNSLLDKE SYIKTYRHIR EDYGRGMIKG WTENSNPQKS IFEDCGIASY INELVNSDLL
PKPNKFVDIG CGNGLLVHLL NKIGMSGYGI DVRHRNIWKT TLKDVDLREM PVDPQIVVQN
EPHFDLDVDL LIGNHSDELT PWIPVMAAKL NCNFFLIPCC PFNFFGKYAN NGSHLGPKRI
VSQYESFFEW TVSVAERLGF DVKIDRLAIP STKRLCIIGR VPEGGLCPNV DETINSMTEG
QKFIARPKEI KTTNCMNIPV TDRERIAKKL FDFVLNYSDA VRDGWRCGGE VPLAQLAALL
TEEDKKLMKD QDGGLQTFLR NQHQIFHVYQ ATARLRDFRQ PVVQKRQSWK PKKAETIRKA
PCWMSLHHPD GCPVGQEACR YEH
