ID   TRM44_CANGA             Reviewed;         564 AA.
AC   Q6FKD6;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=tRNA (uracil-O(2)-)-methyltransferase;
DE            EC=2.1.1.211;
GN   Name=TRM44; OrderedLocusNames=CAGL0L12408g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC       (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC         methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
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DR   EMBL; CR380958; CAG62282.1; -; Genomic_DNA.
DR   RefSeq; XP_449308.1; XM_449308.1.
DR   AlphaFoldDB; Q6FKD6; -.
DR   STRING; 5478.XP_449308.1; -.
DR   EnsemblFungi; CAG62282; CAG62282; CAGL0L12408g.
DR   GeneID; 2890698; -.
DR   KEGG; cgr:CAGL0L12408g; -.
DR   CGD; CAL0135228; CAGL0L12408g.
DR   VEuPathDB; FungiDB:CAGL0L12408g; -.
DR   eggNOG; KOG3790; Eukaryota.
DR   HOGENOM; CLU_018580_2_0_1; -.
DR   InParanoid; Q6FKD6; -.
DR   OMA; WIPLLGY; -.
DR   Proteomes; UP000002428; Chromosome L.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR   GO; GO:0002128; P:tRNA nucleoside ribose methylation; IEA:EnsemblFungi.
DR   InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR   PANTHER; PTHR21210; PTHR21210; 1.
DR   Pfam; PF07757; AdoMet_MTase; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..564
FT                   /note="tRNA (uracil-O(2)-)-methyltransferase"
FT                   /id="PRO_0000249903"
SQ   SEQUENCE   564 AA;  64342 MW;  EC39E4AF4C619FD9 CRC64;
     MTEKKTAPSF QFMKDESTSV LGGEWVPMYC TDEPVEFSKG DFETAMLNVI REPNINSTAI
     LRADILAEKF YDLEGNEQEL STDDKPVLSE SLPYDADAKL VNIDDITTRK ELIENKELEL
     TIVKEFVRRM IPRNPYKDAL INQTCLVYNS KKTENKDTSL VLYIPHFEDE ADCPFYIPHV
     QKVAILLHGG ILSVHYIPYA GQSEDLRKDN ERVVRTAFRL LQTAYKHSKG VMQGYEKRVN
     HDQVVDRVLF QDTYIQLKKK YSKFLVDNWA ESTDPKKHVF EDLAIAAFLL EFWKTQYGDD
     YKTTMQFRDM GCGNGVLTYI LISEGVNGVG IDARKRKSWS IYPTEIQRHL KEQVIIPSIL
     LRPTPDVRRY MPYLEDNGAM FPIKVEDGLI APATLVYSSA DLIASPNVNI CEFPDNTFII
     GNHSDELTCW IPLLGFPFMV IPCCSHDLSG KKVRFNTRVP KSEKTTPAMK NNIKINSNNV
     NNSNSTYAGL VNHVEYLAKK VGWKVEKEML RIPSTRNAAI IGTDNAYAKE FPTPDIYRTI
     EEEGGALTWV QNTMQLAKAS PRNH