ID   TRM44_DEBHA             Reviewed;         594 AA.
AC   Q6BRY1;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=tRNA (uracil-O(2)-)-methyltransferase;
DE            EC=2.1.1.211;
GN   Name=TRM44; OrderedLocusNames=DEHA2D13002g;
OS   Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990
OS   / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX   NCBI_TaxID=284592;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC       (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC         methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
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DR   EMBL; CR382136; CAG87207.2; -; Genomic_DNA.
DR   RefSeq; XP_459039.2; XM_459039.1.
DR   AlphaFoldDB; Q6BRY1; -.
DR   STRING; 4959.XP_459039.2; -.
DR   EnsemblFungi; CAG87207; CAG87207; DEHA2D13002g.
DR   GeneID; 2901524; -.
DR   KEGG; dha:DEHA2D13002g; -.
DR   VEuPathDB; FungiDB:DEHA2D13002g; -.
DR   eggNOG; KOG3790; Eukaryota.
DR   HOGENOM; CLU_018580_2_0_1; -.
DR   InParanoid; Q6BRY1; -.
DR   OMA; WIPLLGY; -.
DR   OrthoDB; 1146281at2759; -.
DR   Proteomes; UP000000599; Chromosome D.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR   InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR   PANTHER; PTHR21210; PTHR21210; 1.
DR   Pfam; PF07757; AdoMet_MTase; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..594
FT                   /note="tRNA (uracil-O(2)-)-methyltransferase"
FT                   /id="PRO_0000249904"
SQ   SEQUENCE   594 AA;  67284 MW;  31009DD72569DCA7 CRC64;
     MVKHTKSKND FDAELLLSET SKIGDPWVPI YQTAVDIEAH HFETAMSNLI KQPNINSTVI
     MRADILKENV FDVENGDSTF VSKLINKVPE FPSENPDDVI LHRYLDDIDL RTISLNNSEL
     KLNTKCEVVR RIIPRNPFKD HIINQTCLVL SSSNADPESH TEKESDDSVL VVYIPHIQSS
     DEIPFYLPPV YGVGILYHKS TLSIQYLPFN YQNYKTSPEI QLSLRNLDVT ERPIRIALRL
     LQTSSKHSLG AKSGYEKRVN HDLVVPKIAF QNRYITLKKK YSSNLVNSWC ESTDPKKHVF
     EDLAIAAFLI ELWTVKYKSR EDFEFRDLGC GNGLLVYILN MEGYSGKGID ARSRKSWSTY
     PENVQNNLSE QIIIPSVLLK PHPALSRLIP NVTDNFRYFQ APQLPSKNNC SCEAENVPAD
     DKSTSHTNSS STLTTYSSAN LLESSQVCTT EEFPPNTFII GNHSDELTCW IPLLGYPFMV
     IPCCSHALSG AKVRYSPRKQ QKSNQQQNVS TYGALVDHTE DLAKQMGWIV EKEMLRIPST
     RNAAIIATKR QPHCIDENDE VTQTRVLDIL ALEGGAEGWV ENSINLMKKA PRNH