ID   TRM44_KLULA             Reviewed;         537 AA.
AC   Q6CR42;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=tRNA (uracil-O(2)-)-methyltransferase;
DE            EC=2.1.1.211;
GN   Name=TRM44; OrderedLocusNames=KLLA0D12012g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC       (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC         methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
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DR   EMBL; CR382124; CAH00693.1; -; Genomic_DNA.
DR   RefSeq; XP_453597.1; XM_453597.1.
DR   AlphaFoldDB; Q6CR42; -.
DR   STRING; 28985.XP_453597.1; -.
DR   EnsemblFungi; CAH00693; CAH00693; KLLA0_D12012g.
DR   GeneID; 2893502; -.
DR   KEGG; kla:KLLA0_D12012g; -.
DR   eggNOG; KOG3790; Eukaryota.
DR   HOGENOM; CLU_018580_2_0_1; -.
DR   InParanoid; Q6CR42; -.
DR   OMA; WIPLLGY; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR   GO; GO:0002128; P:tRNA nucleoside ribose methylation; IEA:EnsemblFungi.
DR   InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR   PANTHER; PTHR21210; PTHR21210; 1.
DR   Pfam; PF07757; AdoMet_MTase; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..537
FT                   /note="tRNA (uracil-O(2)-)-methyltransferase"
FT                   /id="PRO_0000249907"
SQ   SEQUENCE   537 AA;  60886 MW;  1714ABDCC56FC800 CRC64;
     MTSNTLHYTV EKSSILGPEW QSLYSTAEPV DFDKSHFEQA MLNVIRQPNV NSTVILRADM
     LIDNEYDLES GSCVKSEKKD IALGSDEGIL KVNIDDLAPR SVPIALADVT TKFQFVRRIV
     PRNPFKDAII NQTCLVMNSN SDASTSLVIY IPHFDNAELC PFYIPQVAAV GILLHDHQLS
     VHYIPFAGQK ELLFDPKQRV VRTAFRLLQT AYKHSKGVKD GYAKRVMHDV VVDKVLFQDQ
     YIHLKKKYSK FLVDNWAEST DPKKHVFEDI AIAAFLIELW KKIYGAENTT NKMQFRDLGC
     GNGVLCYILI QEGFKGLGID ARQRKSWSIY PAEVKRCLKE QVIIPSVLLR PHPTMKLHAP
     HLQHNGRFFP MQLVSPQLIA PATVMYSSAD LLQSPQVNIA EFPPDTFIIG NHSDELTCWI
     PLLGYPFMVI PCCSHNLSGN RIRYAVRDVE RAKKLGNSTY QGLVDRVEYL ADRVGWKTEK
     EMLRIPSTRN AAIIGYKNDK LNEFPTDEIY SILMEEGGAD GWVANTMNLM KSNPRGH