TRM44_MOUSE
ID TRM44_MOUSE Reviewed; 713 AA.
AC Q9D2Q2; A7MCV4; Q3V1K3;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Probable tRNA (uracil-O(2)-)-methyltransferase;
DE EC=2.1.1.211;
DE AltName: Full=Methyltransferase-like protein 19;
GN Name=Trmt44; Synonyms=Mettl19;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Liver, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
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DR EMBL; AK019101; BAB31547.1; -; mRNA.
DR EMBL; AK132402; BAE21147.1; -; mRNA.
DR EMBL; CH466524; EDL37486.1; -; Genomic_DNA.
DR EMBL; BC152332; AAI52333.1; -; mRNA.
DR CCDS; CCDS19230.1; -.
DR RefSeq; NP_084484.2; NM_030208.3.
DR AlphaFoldDB; Q9D2Q2; -.
DR STRING; 10090.ENSMUSP00000030980; -.
DR iPTMnet; Q9D2Q2; -.
DR PhosphoSitePlus; Q9D2Q2; -.
DR EPD; Q9D2Q2; -.
DR MaxQB; Q9D2Q2; -.
DR PaxDb; Q9D2Q2; -.
DR PeptideAtlas; Q9D2Q2; -.
DR PRIDE; Q9D2Q2; -.
DR ProteomicsDB; 258981; -.
DR Antibodypedia; 43421; 31 antibodies from 12 providers.
DR DNASU; 78890; -.
DR Ensembl; ENSMUST00000030980; ENSMUSP00000030980; ENSMUSG00000029097.
DR GeneID; 78890; -.
DR KEGG; mmu:78890; -.
DR UCSC; uc008xdu.2; mouse.
DR CTD; 152992; -.
DR MGI; MGI:1926140; Trmt44.
DR VEuPathDB; HostDB:ENSMUSG00000029097; -.
DR eggNOG; KOG3790; Eukaryota.
DR GeneTree; ENSGT00390000000645; -.
DR HOGENOM; CLU_021025_1_0_1; -.
DR InParanoid; Q9D2Q2; -.
DR OMA; ECGGIKT; -.
DR OrthoDB; 1146281at2759; -.
DR PhylomeDB; Q9D2Q2; -.
DR TreeFam; TF106127; -.
DR BioGRID-ORCS; 78890; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q9D2Q2; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q9D2Q2; protein.
DR Bgee; ENSMUSG00000029097; Expressed in spermatocyte and 85 other tissues.
DR ExpressionAtlas; Q9D2Q2; baseline and differential.
DR Genevisible; Q9D2Q2; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016300; F:tRNA (uracil) methyltransferase activity; IBA:GO_Central.
DR GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR InterPro; IPR000571; Znf_CCCH.
DR PANTHER; PTHR21210; PTHR21210; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS50103; ZF_C3H1; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Metal-binding; Methyltransferase; Phosphoprotein;
KW Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing;
KW Zinc; Zinc-finger.
FT CHAIN 1..713
FT /note="Probable tRNA (uracil-O(2)-)-methyltransferase"
FT /id="PRO_0000249895"
FT ZN_FING 669..698
FT /note="C3H1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 49..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 480..508
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 76
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IYL2"
FT MOD_RES 489
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IYL2"
FT CONFLICT 511
FT /note="D -> Y (in Ref. 1; BAB31547)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 713 AA; 79836 MW; D95B2353192FF449 CRC64;
MEELGRVRLS DPDALLPAGF WAAVTVWLER PQVANKRLCG ARMEARGRTL RSHAQAECGP
RQGQGHGLER EPGQASPKGE PESGPRASRE GTAPAADLNS LWDRVSQSLV HANPEMLAFL
CGPSLGPQPE AAQELDLILR TVIPKASPHS PLTEPKKELV VQDVSSGSVT FLPLEEDNEG
NLEVKTSNVY QLHLHHNEGE WFISVLIFCP ERWHSDGVVY PKPAWLGEEL LSKLARWAVE
NRKSEFKSTL SLVSILRYSR MYQELKEKYR DMVKVWPEVT DPEKFVYEDV AIATYLLILW
EEERAEKGVT TKQSFVDLGC GNGLLVHILS NEGHPGRGID IRRRKIWDMY GPQTQLEEGS
ITPSDETLFP GVDWLIGNHS DELTPWIPVI AARSSYTCRF FVLPCCFFDF VGRYQRQQSR
KTQYREYLDF VLEVGLSCGF HVQEDCLRIP STKRVCLIGK SRTYPPSAEV WMDEQRTRYL
HSRQGHPQSR PGGAHAPSAP QTAAHDAGLQ DSCRTVNAGS ECVLEGLAAE RGAGAPAPGL
WVPGFCPREK AERVRNCAAL PRDFVDQVVL QVANLLLDRK KFNTGNSEAR SLKPWNGGGS
LSLAEVAAEL NSETLQRLKR ECGGLQTLLK NSHQVFEVLN GRVHIRDWRQ ELQRGKPPEA
KQNLSAAVFK TRICWFFAHH PDGCVLPAAQ CPFAHGPEEL RLSQTLKKQR QAP
