ID   TRM44_NEUCR             Reviewed;         505 AA.
AC   Q7SE80; Q6MVC8;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-MAR-2014, sequence version 2.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=tRNA (uracil-O(2)-)-methyltransferase;
DE            EC=2.1.1.211;
DE   AltName: Full=tRNA(M-2-U44)-methyltransferase;
GN   Name=trm44; ORFNames=B13D15.160, NCU01980;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC       (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC         methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAE76371.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; BX842630; CAE76371.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CM002236; EAA35108.3; -; Genomic_DNA.
DR   RefSeq; XP_964344.3; XM_959251.3.
DR   AlphaFoldDB; Q7SE80; -.
DR   STRING; 5141.EFNCRP00000001098; -.
DR   EnsemblFungi; EAA35108; EAA35108; NCU01980.
DR   GeneID; 3880484; -.
DR   KEGG; ncr:NCU01980; -.
DR   VEuPathDB; FungiDB:NCU01980; -.
DR   HOGENOM; CLU_018580_2_1_1; -.
DR   InParanoid; Q7SE80; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016300; F:tRNA (uracil) methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002128; P:tRNA nucleoside ribose methylation; IEA:EnsemblFungi.
DR   InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR   PANTHER; PTHR21210; PTHR21210; 1.
DR   Pfam; PF07757; AdoMet_MTase; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..505
FT                   /note="tRNA (uracil-O(2)-)-methyltransferase"
FT                   /id="PRO_0000249908"
SQ   SEQUENCE   505 AA;  56849 MW;  B7C1F1B5BA9F5717 CRC64;
     MPKFTPEELA VNAVPVIRQA TSGDAEPDWR PLFRHECTFN PVIFQDVMLN LIKNPNINSS
     WLFRADILYD TEKNIQNGLE TPLSELGDVP DIPSFDGFGL HRRLIRRLIP RSTMRDKPMD
     QTCLIYQSKG SDSEGGLQRH LVIYLPHVST EAEMPYYHPT VRGIAFHHEW SPTETRGSVS
     VSYLYFEGGE RPEKLTRIAS HLLQAVYKHG QGRADGYQKR VHHDLLVPQD RVQNTYTALK
     QKYARALIES WVESTDPTKH VFEDLCIAAF LIELWTDIYG RNFFPGFVDI GCGNGLLVHI
     LNLEGFKGWG FDARSRKSWA TYSTRLETPT GVQESLQQLV LLPPPVSRAG ISEISSEGFR
     EELVHDGRFP KGTFIISNHA DELTPWTPIL AAISDCPFIM IPCCSHDLTG ARFRAPAPKD
     KKKANSAYSS LVSWVTQITA DCGWVAEQEM LRIPSTRNTA IVGRKRQGDV SAIDIGVIVD
     RYGGTAGYLE TVVKLVKSTN LDEKH