ID   TRM44_SCHPO             Reviewed;         502 AA.
AC   O74516;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=tRNA (uracil-O(2)-)-methyltransferase;
DE            EC=2.1.1.211;
GN   Name=trm44; ORFNames=SPCC663.10;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC       (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC         methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CU329672; CAA20370.1; -; Genomic_DNA.
DR   PIR; T41541; T41541.
DR   RefSeq; NP_588271.1; NM_001023261.2.
DR   AlphaFoldDB; O74516; -.
DR   BioGRID; 275289; 2.
DR   STRING; 4896.SPCC663.10.1; -.
DR   MaxQB; O74516; -.
DR   PaxDb; O74516; -.
DR   EnsemblFungi; SPCC663.10.1; SPCC663.10.1:pep; SPCC663.10.
DR   GeneID; 2538705; -.
DR   KEGG; spo:SPCC663.10; -.
DR   PomBase; SPCC663.10; trm44.
DR   VEuPathDB; FungiDB:SPCC663.10; -.
DR   eggNOG; KOG3790; Eukaryota.
DR   HOGENOM; CLU_018580_2_0_1; -.
DR   InParanoid; O74516; -.
DR   OMA; WIPLLGY; -.
DR   PhylomeDB; O74516; -.
DR   PRO; PR:O74516; -.
DR   Proteomes; UP000002485; Chromosome III.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0016300; F:tRNA (uracil) methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR   GO; GO:0030488; P:tRNA methylation; ISO:PomBase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR   PANTHER; PTHR21210; PTHR21210; 1.
DR   Pfam; PF07757; AdoMet_MTase; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..502
FT                   /note="tRNA (uracil-O(2)-)-methyltransferase"
FT                   /id="PRO_0000249909"
SQ   SEQUENCE   502 AA;  58318 MW;  75CAD9D806845811 CRC64;
     MKVPAYWKPR EPKNALTCDP PVPYDLQSSY QWQSILEHDT CYAAEVFDKM MIELVYHPER
     TSSVIMRTDI ILDSQEDDSI LNKQKSVFEN LDERYQISRW IDRRIIPRNT NLDATMDQTV
     VVLHEKLEND RILMYLPHLD KSLEQPYNHL PYYHPAVAGI AFHYKGSSVE VLYLVEKDFQ
     RELSSRLLRT AHMLLLVLHK HCKGAVEGYQ KRMLHDTVVE RNKFQDTYVI LKDKYAKQLV
     DNWVEKTDPG KHVFEDLAIA AFLIELWKQT YSSNKEFSFV DVGCGNGLLV YLLLMEGYNG
     YGFDARKRKS WETYPLWVQV KLYEKVLVPY FLHDFETKIP FPQLPAGFTM SAANIHDGRF
     SENSFLIGNH ADELTPYLPI LARLNQKCYF MSIPCCVHDL TGAKISWALP KPRNPKHGGR
     YAMYIEWLMQ LSEEVGWNIE VEPLRIPSTR NYALIGRKLL SNDLLHSSLS TDALYDIVEK
     NHGSQGFFVH AMQVAAANSR SH