TRM44_XENLA
ID TRM44_XENLA Reviewed; 668 AA.
AC Q4KLT3; Q7ZY77;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Probable tRNA (uracil-O(2)-)-methyltransferase;
DE EC=2.1.1.211;
DE AltName: Full=Methyltransferase-like protein 19;
GN Name=trmt44; Synonyms=mettl19;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Egg, and Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH43909.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR EMBL; BC043909; AAH43909.1; ALT_SEQ; mRNA.
DR EMBL; BC099012; AAH99012.1; -; mRNA.
DR RefSeq; NP_001082524.1; NM_001089055.1.
DR AlphaFoldDB; Q4KLT3; -.
DR PRIDE; Q4KLT3; -.
DR DNASU; 398534; -.
DR GeneID; 398534; -.
DR KEGG; xla:398534; -.
DR CTD; 398534; -.
DR Xenbase; XB-GENE-991929; trmt44.L.
DR OrthoDB; 1146281at2759; -.
DR Proteomes; UP000186698; Chromosome 1L.
DR Bgee; 398534; Expressed in egg cell and 19 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR InterPro; IPR000571; Znf_CCCH.
DR PANTHER; PTHR21210; PTHR21210; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
DR PROSITE; PS50103; ZF_C3H1; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Metal-binding; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase; tRNA processing; Zinc; Zinc-finger.
FT CHAIN 1..668
FT /note="Probable tRNA (uracil-O(2)-)-methyltransferase"
FT /id="PRO_0000249896"
FT ZN_FING 620..649
FT /note="C3H1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 441..460
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 143
FT /note="T -> K (in Ref. 1; AAH43909)"
FT /evidence="ECO:0000305"
FT CONFLICT 429
FT /note="R -> Q (in Ref. 1; AAH43909)"
FT /evidence="ECO:0000305"
FT CONFLICT 531
FT /note="K -> R (in Ref. 1; AAH43909)"
FT /evidence="ECO:0000305"
FT CONFLICT 560
FT /note="A -> T (in Ref. 1; AAH43909)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 668 AA; 76767 MW; 4C309BD6A0888D54 CRC64;
MPELASVLVR DERSVLPGGF WAAVAVWLEK PQVLNKRLCG STVGKKWDHV GEESESEGID
VETALAALLE DMSGPGRDEE MLKLISGYGK REEDSGSLTL RTLIPKSNPH FPSCQPRCEI
VIRDLPNSVV TFLPLDEANQ FKTNNIYQIR LAHVQDDEWS ISLYTLCSEA WESDGVVYPK
ITWMKNELLS KLAKWSTEDK KSEFKSTLSL VPVDKYSRLY QNLKEKYRDM VKVWPEVTDP
EKFVYEDVAI ATYLLIIWEE ERSQNQLKVK QSFVDLGCGN GLLVHILSNE GHPGRGIDVR
RRKIWDMYGP QTILEESAIL PNDDYLFPDT DWLIGNHSDE LTPWLPVIAS RSSYSCRYFV
LPCCFFNFYG KYNRKSSKKT QYREYLDFVK EVGQQCGFNV EEDCLRIPST KRVCLIGMSR
TYPLTQEKRI DEQRTRYIKS QHTDSLHIST KSSLDKDDPP PLNCHAKNGQ VDNVDSSLVQ
SEKLPGSWMP GFQPREKEEP IRNCALLPRD FIDETVLQVA KLLLYSNTKE KETPITDQSE
TVWNTGRSLT LREVAEHLHA DTLQKLKNEC GGLQTLLRNN HQVFKVINSN VSIRDWQKDT
PTNTKKRKPE AKRTVPSDVL KTRLCWFYVH HPNGCPRVAK SCPYAHGAEE LRPSFISRRK
NPRNKLCT