ID   TRM44_YARLI             Reviewed;         529 AA.
AC   Q6C7U7;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=tRNA (uracil-O(2)-)-methyltransferase;
DE            EC=2.1.1.211;
GN   Name=TRM44; OrderedLocusNames=YALI0D25212g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC       (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC         methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
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DR   EMBL; CR382130; CAG81469.1; -; Genomic_DNA.
DR   RefSeq; XP_503265.1; XM_503265.1.
DR   AlphaFoldDB; Q6C7U7; -.
DR   STRING; 4952.CAG81469; -.
DR   EnsemblFungi; CAG81469; CAG81469; YALI0_D25212g.
DR   GeneID; 2910783; -.
DR   KEGG; yli:YALI0D25212g; -.
DR   VEuPathDB; FungiDB:YALI0_D25212g; -.
DR   HOGENOM; CLU_018580_2_0_1; -.
DR   InParanoid; Q6C7U7; -.
DR   OMA; WIPLLGY; -.
DR   Proteomes; UP000001300; Chromosome D.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016300; F:tRNA (uracil) methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IEA:RHEA.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002128; P:tRNA nucleoside ribose methylation; IEA:EnsemblFungi.
DR   InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR   PANTHER; PTHR21210; PTHR21210; 1.
DR   Pfam; PF07757; AdoMet_MTase; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..529
FT                   /note="tRNA (uracil-O(2)-)-methyltransferase"
FT                   /id="PRO_0000249910"
FT   REGION          78..107
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        78..98
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   529 AA;  60003 MW;  192FD079AE005016 CRC64;
     MFTPTDISGE PSAVGTQWTP YLSHPVDFGA QHFLEAMLNV ISQPNINSSV IMRADIISDS
     LNMMPNIMGT DKANEGFIFD THEGPVDDDK KDTDKDKNTP VPPNLQDYEP RQVEVTPCPV
     KRVIVRRIIP RNPQNDFSVN QTCIVHSDKP LWDSDGKETS EGAKIAISYI SHHDHPKSCP
     YYLPCVKAVL LYFDGSAISV YYEKYTDYEL SAEQEERCGR IALHLLHTVF RHSSGQKAGY
     KKRVHHDMII DRIKFQDRYI YLKQKYAHSL VSSWVESTDP RKHVFEDLAI AAFLIELWGQ
     MYKNKDDIYF YDLGCGNGLL VNILIKEGYV GEGVDARARK SWQTYEPEVT QKLLEKIVVP
     TVLLDQMVGQ AQYLPPHAAE KRLAEANGLA TDPRVFQTAG LPENAFLIGN HSDELTCWIP
     LMDRPFMVIP CCSHALSGEK KRFPPTSSDP EEKSTYRSLV GHVEQLSSKI GWQVEKEYLR
     IPSTRNAAVI GRTRVEPQMN IFEILYSEGG GEGWVERARD LCAKSPRNH