BUN1_DROME
ID BUN1_DROME Reviewed; 219 AA.
AC Q24522; E1JH96; E1JHG7; O02431; Q0E8Q8; Q0E8Q9; Q95T76;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Protein bunched, class 1/class 3/D/E isoforms;
DE AltName: Full=Protein shortsighted;
GN Name=bun; Synonyms=shs; ORFNames=CG42281;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CLASS 1), ALTERNATIVE SPLICING,
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC TISSUE=Eye-antennal disk;
RX PubMed=7555710; DOI=10.1242/dev.121.9.2835;
RA Treisman J.E., Lai Z.-C., Rubin G.M.;
RT "Shortsighted acts in the decapentaplegic pathway in Drosophila eye
RT development and has homology to a mouse TGF-beta-responsive gene.";
RL Development 121:2835-2845(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CLASS 1), FUNCTION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Ovary;
RX PubMed=9256356; DOI=10.1016/s0925-4773(97)00080-4;
RA Dobens L., Hsu T., Twombly V., Gelbart W.M., Raftery L.A., Kafatos F.C.;
RT "The Drosophila bunched gene is a homologue of the growth factor stimulated
RT mammalian TSC-22 sequence and is required during oogenesis.";
RL Mech. Dev. 65:197-208(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CLASS 3).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Probable transcription factor required for peripheral nervous
CC system morphogenesis, eye development and oogenesis. May be required
CC for the transmission of the dpp signal and for a morphogenetic movement
CC of the medulla in the brain that reorients the second optic lobe
CC relative to the first. Plays a role in determining proper dorsal cell
CC fates leading to the formation of the dorsal appendages.
CC {ECO:0000269|PubMed:7555710, ECO:0000269|PubMed:9256356}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:7555710}. Nucleus
CC {ECO:0000269|PubMed:7555710}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=7;
CC Name=Class 1; Synonyms=B;
CC IsoId=Q24522-1; Sequence=Displayed;
CC Name=Class 3; Synonyms=C;
CC IsoId=Q24522-2, Q24523-2;
CC Sequence=VSP_010282;
CC Name=D; Synonyms=H;
CC IsoId=Q24522-3; Sequence=VSP_035860;
CC Name=E;
CC IsoId=Q24522-4; Sequence=VSP_035859;
CC Name=Class 2; Synonyms=A;
CC IsoId=Q24523-1; Sequence=External;
CC Name=F;
CC IsoId=Q24523-3; Sequence=External;
CC Name=G;
CC IsoId=Q24523-4; Sequence=External;
CC -!- TISSUE SPECIFICITY: Anterior dorsal follicle cells at stages 10-12 of
CC oogenesis. {ECO:0000269|PubMed:9256356}.
CC -!- SIMILARITY: Belongs to the TSC-22/Dip/Bun family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB61239.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; L42511; AAC41607.1; -; mRNA.
DR EMBL; AF003342; AAB61239.1; ALT_INIT; mRNA.
DR EMBL; AE014134; AAF53200.3; -; Genomic_DNA.
DR EMBL; AE014134; AAN10817.1; -; Genomic_DNA.
DR EMBL; AE014134; ABI31312.1; -; Genomic_DNA.
DR EMBL; AE014134; ACZ94250.1; -; Genomic_DNA.
DR EMBL; AE014134; ACZ94251.1; -; Genomic_DNA.
DR EMBL; AY060295; AAL25334.1; -; mRNA.
DR RefSeq; NP_001036358.1; NM_001042893.2. [Q24522-4]
DR RefSeq; NP_001162964.1; NM_001169493.2. [Q24522-3]
DR RefSeq; NP_001162965.1; NM_001169494.2. [Q24522-1]
DR RefSeq; NP_723755.2; NM_165012.2. [Q24522-3]
DR RefSeq; NP_723756.1; NM_165013.4. [Q24522-2]
DR AlphaFoldDB; Q24522; -.
DR SMR; Q24522; -.
DR BioGRID; 60711; 32.
DR IntAct; Q24522; 7.
DR DNASU; 34665; -.
DR EnsemblMetazoa; FBtr0299652; FBpp0288927; FBgn0259176. [Q24522-3]
DR EnsemblMetazoa; FBtr0299656; FBpp0288931; FBgn0259176. [Q24522-1]
DR EnsemblMetazoa; FBtr0299657; FBpp0288932; FBgn0259176. [Q24522-2]
DR EnsemblMetazoa; FBtr0300522; FBpp0289749; FBgn0259176. [Q24522-4]
DR EnsemblMetazoa; FBtr0300524; FBpp0289751; FBgn0259176. [Q24522-3]
DR GeneID; 34665; -.
DR CTD; 34665; -.
DR FlyBase; FBgn0259176; bun.
DR VEuPathDB; VectorBase:FBgn0259176; -.
DR GeneTree; ENSGT00940000168943; -.
DR BioGRID-ORCS; 34665; 1 hit in 3 CRISPR screens.
DR ChiTaRS; bun; fly.
DR GenomeRNAi; 34665; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0259176; Expressed in brain and 47 other tissues.
DR ExpressionAtlas; Q24522; baseline and differential.
DR Genevisible; Q24522; DM.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:FlyBase.
DR GO; GO:0007304; P:chorion-containing eggshell formation; IMP:FlyBase.
DR GO; GO:0001751; P:compound eye photoreceptor cell differentiation; IMP:FlyBase.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:FlyBase.
DR GO; GO:0046843; P:dorsal appendage formation; IMP:FlyBase.
DR GO; GO:0036335; P:intestinal stem cell homeostasis; IMP:FlyBase.
DR GO; GO:0016319; P:mushroom body development; IMP:FlyBase.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:FlyBase.
DR GO; GO:0009996; P:negative regulation of cell fate specification; IMP:FlyBase.
DR GO; GO:0007297; P:ovarian follicle cell migration; IMP:FlyBase.
DR GO; GO:0030307; P:positive regulation of cell growth; IMP:FlyBase.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:FlyBase.
DR GO; GO:0002052; P:positive regulation of neuroblast proliferation; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0042246; P:tissue regeneration; IMP:FlyBase.
DR InterPro; IPR000580; TSC-22_Dip_Bun.
DR Pfam; PF01166; TSC22; 1.
DR PROSITE; PS01289; TSC22; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW Nucleus; Oogenesis; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..219
FT /note="Protein bunched, class 1/class 3/D/E isoforms"
FT /id="PRO_0000219376"
FT REGION 82..103
FT /note="Leucine-zipper"
FT VAR_SEQ 1..64
FT /note="MKTETGSNNNNTTVVNMDFDMYPSISGKQQDPVREVVMKYIDYFLPDASGTS
FT AVAIDNKIEQAM -> MMGTMSLRQPENWLYEIWQKGYDSPCYVMVPNAK (in
FT isoform Class 3)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_010282"
FT VAR_SEQ 1..47
FT /note="MKTETGSNNNNTTVVNMDFDMYPSISGKQQDPVREVVMKYIDYFLPD -> M
FT AALANKMAANMATTSSSSNNNNIAGSESTLQQQQMLHHQQQQQQQTQSNLIMPAAVIQP
FT EYLLNNDRGLFKVYCFIKDFIG (in isoform E)"
FT /evidence="ECO:0000305"
FT /id="VSP_035859"
FT VAR_SEQ 2..47
FT /note="KTETGSNNNNTTVVNMDFDMYPSISGKQQDPVREVVMKYIDYFLPD -> IK
FT YPRTTSSTSSGYFDDDEAMMNPYQTPPASPQSVPQYLQRHMVRMFSTEVDN (in
FT isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_035860"
FT CONFLICT 30
FT /note="Q -> E (in Ref. 2; AAB61239)"
FT /evidence="ECO:0000305"
FT CONFLICT 34
FT /note="R -> C (in Ref. 2; AAB61239)"
FT /evidence="ECO:0000305"
FT CONFLICT 100
FT /note="N -> H (in Ref. 2; AAB61239)"
FT /evidence="ECO:0000305"
FT CONFLICT 106
FT /note="N -> K (in Ref. 2; AAB61239)"
FT /evidence="ECO:0000305"
FT CONFLICT 122..123
FT /note="AA -> PR (in Ref. 2; AAB61239)"
FT /evidence="ECO:0000305"
FT CONFLICT 188
FT /note="E -> G (in Ref. 2; AAB61239)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="V -> G (in Ref. 2; AAB61239)"
FT /evidence="ECO:0000305"
FT CONFLICT 202..203
FT /note="TS -> QQVTSAA (in Ref. 1; AAC41607 and 2; AAB61239)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 219 AA; 22742 MW; 29B3C20698C38743 CRC64;
MKTETGSNNN NTTVVNMDFD MYPSISGKQQ DPVREVVMKY IDYFLPDASG TSAVAIDNKI
EQAMDLVKSH LMIAVREEVE VLKERISELM DKINKLELEN SILKSNIPQE TLQQLQLQLQ
LAAPPATPAI QAAPAVQSVV APAAAGQAVQ QQAAGAVAVT GVATSPASAV VPTSIPNGSA
ENGSSAVESA AVSVEQQVQQ VTSAAAAAAS VVTANGPMS
