BUN2_DROME
ID BUN2_DROME Reviewed; 1331 AA.
AC Q24523; D4G7A9; Q0E8R0; Q9VK78; Q9VK79;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2012, sequence version 4.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Protein bunched, class 2/F/G isoform;
DE AltName: Full=Protein shortsighted;
GN Name=bun; Synonyms=shs; ORFNames=CG42281;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CLASS 2), FUNCTION, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Eye-antennal disk;
RX PubMed=7555710; DOI=10.1242/dev.121.9.2835;
RA Treisman J.E., Lai Z.-C., Rubin G.M.;
RT "Shortsighted acts in the decapentaplegic pathway in Drosophila eye
RT development and has homology to a mouse TGF-beta-responsive gene.";
RL Development 121:2835-2845(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 462-1331 (ISOFORM F).
RC STRAIN=Berkeley; TISSUE=Testis;
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable transcription factor required for peripheral nervous
CC system morphogenesis, eye development and oogenesis. May be required
CC for the transmission of the dpp signal and for a morphogenetic movement
CC of the medulla in the brain that reorients the second optic lobe
CC relative to the first. Plays a role in determining proper dorsal cell
CC fates leading to the formation of the dorsal appendages.
CC {ECO:0000269|PubMed:7555710}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:7555710}. Nucleus
CC {ECO:0000269|PubMed:7555710}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=7;
CC Name=G;
CC IsoId=Q24523-4; Sequence=Displayed;
CC Name=Class 2; Synonyms=A;
CC IsoId=Q24523-1; Sequence=VSP_043733, VSP_043734;
CC Name=F;
CC IsoId=Q24523-3; Sequence=VSP_043734;
CC Name=Class 1; Synonyms=B;
CC IsoId=Q24522-1; Sequence=External;
CC Name=Class 3; Synonyms=C;
CC IsoId=Q24522-2, Q24523-2;
CC Sequence=External;
CC Name=D; Synonyms=H;
CC IsoId=Q24522-3; Sequence=External;
CC Name=E;
CC IsoId=Q24522-4; Sequence=External;
CC -!- SIMILARITY: Belongs to the TSC-22/Dip/Bun family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC41608.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; L42512; AAC41608.1; ALT_INIT; mRNA.
DR EMBL; AE014134; AAF53201.2; -; Genomic_DNA.
DR EMBL; AE014134; ABI31311.2; -; Genomic_DNA.
DR EMBL; AE014134; ABI31313.1; -; Genomic_DNA.
DR EMBL; BT122061; ADD74346.1; -; mRNA.
DR PIR; T13804; T13804.
DR RefSeq; NP_001036357.2; NM_001042892.3. [Q24523-4]
DR RefSeq; NP_001036359.1; NM_001042894.2. [Q24523-3]
DR RefSeq; NP_525103.2; NM_080364.4. [Q24523-1]
DR AlphaFoldDB; Q24523; -.
DR SMR; Q24523; -.
DR BioGRID; 60711; 32.
DR IntAct; Q24523; 2.
DR STRING; 7227.FBpp0288933; -.
DR PaxDb; Q24523; -.
DR PRIDE; Q24523; -.
DR DNASU; 34665; -.
DR EnsemblMetazoa; FBtr0299658; FBpp0288933; FBgn0259176. [Q24523-4]
DR EnsemblMetazoa; FBtr0300521; FBpp0289748; FBgn0259176. [Q24523-1]
DR EnsemblMetazoa; FBtr0300523; FBpp0289750; FBgn0259176. [Q24523-3]
DR GeneID; 34665; -.
DR KEGG; dme:Dmel_CG42281; -.
DR CTD; 34665; -.
DR FlyBase; FBgn0259176; bun.
DR VEuPathDB; VectorBase:FBgn0259176; -.
DR eggNOG; KOG4797; Eukaryota.
DR GeneTree; ENSGT00940000168943; -.
DR InParanoid; Q24523; -.
DR OMA; EDSGHQQ; -.
DR PhylomeDB; Q24523; -.
DR BioGRID-ORCS; 34665; 1 hit in 3 CRISPR screens.
DR ChiTaRS; bun; fly.
DR GenomeRNAi; 34665; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0259176; Expressed in brain and 47 other tissues.
DR ExpressionAtlas; Q24523; baseline and differential.
DR Genevisible; Q24523; DM.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:FlyBase.
DR GO; GO:0007304; P:chorion-containing eggshell formation; IMP:FlyBase.
DR GO; GO:0001751; P:compound eye photoreceptor cell differentiation; IMP:FlyBase.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:FlyBase.
DR GO; GO:0046843; P:dorsal appendage formation; IMP:FlyBase.
DR GO; GO:0036335; P:intestinal stem cell homeostasis; IMP:FlyBase.
DR GO; GO:0016319; P:mushroom body development; IMP:FlyBase.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:FlyBase.
DR GO; GO:0009996; P:negative regulation of cell fate specification; IMP:FlyBase.
DR GO; GO:0007297; P:ovarian follicle cell migration; IMP:FlyBase.
DR GO; GO:0030307; P:positive regulation of cell growth; IMP:FlyBase.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:FlyBase.
DR GO; GO:0002052; P:positive regulation of neuroblast proliferation; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR GO; GO:0042246; P:tissue regeneration; IMP:FlyBase.
DR InterPro; IPR000580; TSC-22_Dip_Bun.
DR Pfam; PF01166; TSC22; 1.
DR PROSITE; PS01289; TSC22; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..1331
FT /note="Protein bunched, class 2/F/G isoform"
FT /id="PRO_0000219377"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 101..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 429..471
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 553..582
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 597..665
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 681..750
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 814..853
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 908..950
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1194..1215
FT /note="Leucine-zipper"
FT COMPBIAS 9..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..190
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..212
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..231
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..273
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 429..447
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 553..577
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 622..661
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 908..937
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1
FT /note="M -> MAENQSAASKDSGHQQQHQQQQQQQHQQHQQPLATTSVTAASTTSVL
FT ANQSPTNSQASSPENSQEALPLLRRQQSAAAATVAAAAATVAATTSGTSQQQQQQHRNS
FT ISNM (in isoform Class 2)"
FT /evidence="ECO:0000303|PubMed:7555710"
FT /id="VSP_043733"
FT VAR_SEQ 926..1159
FT /note="Missing (in isoform Class 2 and isoform F)"
FT /evidence="ECO:0000303|PubMed:7555710, ECO:0000303|Ref.4"
FT /id="VSP_043734"
FT CONFLICT 219
FT /note="Missing (in Ref. 1; AAC41608)"
FT /evidence="ECO:0000305"
FT CONFLICT 575
FT /note="A -> V (in Ref. 4; ADD74346)"
FT /evidence="ECO:0000305"
FT CONFLICT 691
FT /note="Q -> QQQ (in Ref. 1; AAC41608 and 4; ADD74346)"
FT /evidence="ECO:0000305"
FT CONFLICT 1314..1315
FT /note="TS -> QQVTSAA (in Ref. 1; AAC41608)"
FT /evidence="ECO:0000305"
FT CONFLICT 1321
FT /note="A -> AAA (in Ref. 4; ADD74346)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1331 AA; 138064 MW; 3BA21336734B4A46 CRC64;
MFDRTVNAKF KPASSNAGPG NNPVRRNSML TPSRGVTIGG TGGNIRKLTK VSSLTSNHHF
AVCYPPSNIY QNSNNAGSNS ALQRTTSESL RLNMMSRVAA GATPTTVSRA SSNSSLATST
STSLAPKSSS SSGGSNSTPQ QQQQQLVSSN NSSSSSNNSF TKASSPNNNG ARSVGGAATS
AATGTTAAAG SHHHQPHHHH HHHHHHHQHH NHQQQQQQQT SLSQGHASLT VAGGSASAGG
GGGGGSGSSS GTAAGGTNRK PKTTSSFEIT SVTVGHPKLN AAGDTGDESA DDLDESHTDD
NSRITDLENE TPSMSEDTFS KEEVYYANNA LSTNAPVIPT SSQYGLVVVD PIAPSLGQTI
QNVQVNVSDN IINVVSGAVT PGGTKKKDDI KETQHRSERF KVVKIESTEP FKRGRWMCMD
YLDHSSVGNG GNNNEKTGSS TSEAHAATTD GGAAGVGAGS EAPAHKTTQS MILPPTQKLN
ENHLEANSTD ANWNYAEQQQ QQQQQQQQQQ TIVGNALTKT LPVALRNVSR SSSVTRSPNA
TVEFLSPNLL AQQQQQQQQL FDSVNANAAS SPNPAGDPNN MDYARTAAMQ LHQTLQQLKQ
REDAMDVPPG AGGYANYQNG GDSAVGAASN NNSAAAATGE SQLSTSYVEQ QQQQQQPLSP
APLTPQAAPT FAAVAAGQSP NFQLEQQQQQ QQATSQIDGI VPQPFNPQQQ QQQTPQQSTA
QQAAAAANAT SAVTAPPPQQ TSNTSNAAVT TGQGQTMPLL SHMTSYEQQQ PNLGAAAAAA
AAGGTAATSV AAPQAIPTLQ LQSAPSTIAD PQQLMVPQQQ QQQQHQEEQQ QQPQQQQQPL
PPANIASASA NNSNLNLTNT NVVATGEATT NALTLTDEQA TAALAAAFAT GAAAAATGAT
SAAAATQQQI QQLQQQPNAE SETESFITAS NPGGNRKRAF SNPHIGGPND PSFMHRLNPQ
LYYYNKSQSG RSSFCVDESL WPTNNPNGAV SDTNLYMGSS TDEDYEEAID QFSPTIYTTR
SASRAIPIPN SAGSSPQHQM HHSQPRIAIG INQMEGGGPT RSTGAFSASP SIYAYPHSPF
YASSPETSFG SAAMPGHPAA SSRISFSYDP AFQRLQVPNA SGDRRPRSPL ECASVFAAVA
AAATCGDAAG GAADTVISSA SGTSAVAIDN KIEQAMDLVK SHLMIAVREE VEVLKERISE
LMDKINKLEL ENSILKSNIP QETLQQLQLQ LQLAAPPATP AIQAAPAVQS VVAPAAAGQA
VQQQAAGAVA VTGVATSPAS AVVPTSIPNG SAENGSSAVE SAAVSVEQQV QQVTSAAAAA
ASVVTANGPM S
